Recombinant Human Cytochrome P450 1B1 Expression inEscherichia coli

Experiments in molecular genetics

1972 • 28,260 citations

The Carbon Monoxide-binding Pigment of Liver Microsomes

1964 • 11,832 citations

Silver staining of proteins in polyacrylamide gels

1981 • 3,565 citations

P450 superfamily: update on new sequences, gene mapping, accession numbers and nomenclature

1996 • 2,895 citations

On Finding All Suboptimal Foldings of an RNA Molecule

1989 • 2,019 citations

The P450 Superfamily: Update on New Sequences, Gene Mapping, Accession Numbers, Early Trivial Names of Enzymes, and Nomenclature

1993 • 1,663 citations

Principles and Methods of Toxicology

1984 • 1,459 citations

Hepatic Triphosphopyridine Nucleotide-Cytochrome c Reductase: Isolation, Characterization, and Kinetic Studies

1962 • 1,431 citations

Some properties of a detergent-solubilized NADPH-cytochrome c(cytochrome P-450) reductase purified by biospecific affinity chromatography.

1976 • 1,180 citations

Activation of chemically diverse procarcinogens by human cytochrome P-450 1B1.

1996 • 793 citations

Molecular Mechanisms of Estrogen Carcinogenesis

1996 • 659 citations

Identification of three different truncating mutations in cytochrome P4501B1 (CYP1B1) as the principal cause of primary congenital glaucoma (Buphthalmos) in families linked to the GLC3A locus on chromosome 2p21

1997 • 630 citations

17 beta-estradiol hydroxylation catalyzed by human cytochrome P450 1B1.

1996 • 621 citations

Expression and enzymatic activity of recombinant cytochrome P450 17 alpha-hydroxylase in Escherichia coli.

1991 • 547 citations

Preparation and Properties of Partially Purified Cytochrome P-450 and Reduced Nicotinamide Adenine Dinucleotide Phosphate-Cytochrome P-450 Reductase from Rabbit Liver Microsomes

1974 • 518 citations

Complete cDNA sequence of a human dioxin-inducible mRNA identifies a new gene subfamily of cytochrome P450 that maps to chromosome 2.

1994 • 503 citations

A Practical Guide to Protein and Peptide Purification for Microsequencing

1993 • 497 citations

Estimation of isozymes of microsomal cytochrome P-450 in rats, rabbits, and humans using immunochemical staining coupled with sodium dodecyl sulfate-polyacrylamide gel electrophoresis

1982 • 422 citations

4-Hydroxylation of estrogens as marker of human mammary tumors.

1996 • 411 citations

Principles and methods of toxicology

1995 • 407 citations

[17] Predicting optimal and suboptimal secondary structure for RNA

1990 • 383 citations

Expression of Modified Human Cytochrome P450 3A4 in Escherichia coli and Purification and Reconstitution of the Enzyme

1993 • 341 citations

Sequence determinants of cytosolic N-terminal protein processing

1986 • 304 citations

Drug metabolism by Escherichia coli expressing human cytochromes P450

1997 • 281 citations

Human liver microsomal cytochrome P-450 enzymes involved in the bioactivation of procarcinogens detected by umu gene response in Salmonella typhimurium TA 1535/pSK1002.

1989 • 265 citations

Human liver microsomal cytochrome P-450 mephenytoin 4-hydroxylase, a prototype of genetic polymorphism in oxidative drug metabolism. Purification and characterization of two similar forms involved in the reaction.

1986 • 258 citations

Methionine as translation start signal: A review of the enzymes of the pathway in Escherichia coli

1993 • 220 citations

Inherent specificities of purified cytochromes P-450 and P-448 toward biphenyl hydroxylation and ethoxyresorufin deethylation.

1975 • 209 citations

4-Hydroxylation of estradiol by human uterine myometrium and myoma microsomes: implications for the mechanism of uterine tumorigenesis.

1995 • 205 citations

Expression of Modified Human Cytochrome P450 1A2 in Escherichia coli: Stabilization, Purification, Spectral Characterization, and Catalytic Activities of the Enzyme

1994 • 186 citations

Roles of Cytochromes P450 1A2 and 3A4 in the Oxidation of Estradiol and Estrone in Human Liver Microsomes

1998 • 176 citations

Importance of the Proline-Rich Region Following Signal-Anchor Sequence in the Formation of Correct Conformation of Microsomal Cytochrome P-450s1

1993 • 169 citations

Identification of a Rat Adrenal Cytochrome P450 Active in Polycyclic Hydrocarbon Metabolism as Rat CYP1B1

1995 • 169 citations

Oxidation of xenobiotics by recombinant human cytochrome P450 1B1.

1997 • 166 citations

Kinetics of Ferric Cytochrome P450 Reduction by NADPH−Cytochrome P450 Reductase: Rapid Reduction in the Absence of Substrate and Variations among Cytochrome P450 Systems

1997 • 166 citations

[8] Purification of cytochrome b5

1978 • 141 citations

Reconstitution of Recombinant Cytochrome P450 2C10(2C9) and Comparison with Cytochrome P450 3A4 and Other Forms: Effects of Cytochrome P450–P450 and Cytochrome P450–b5Interactions

1997 • 124 citations

Expression of Modified Cytochrome P450 2C10 (2C9) in Escherichia coli, Purification, and Reconstitution of Catalytic Activity

1993 • 116 citations

High‐level expression of functional human cytochrome P450 1A2 in Escherichia coli

1992 • 111 citations

Expression of Modified Human Cytochrome P450 1A1 in Escherichia coli: Effects of 5′ Substitution, Stabilization, Purification, Spectral Characterization, and Catalytic Properties

1994 • 108 citations

New Applications of Bacterial Systems to Problems in Toxicology

1996 • 106 citations

Expression of Cytochrome P450 2D6 in Escherichia coli, Purification, and Spectral and Catalytic Characterization

1995 • 104 citations

Identification of molecular aggregates containing glycoproteins III, J, K (carboxypeptidase H), and H (Kex2-related proteases) in the soluble and membrane fractions of adrenal medullary chromaffin granules.

1992 • 87 citations

Biological Oxidations and P450 Reactions

1997 • 67 citations

Inhibition of human cytochrome P450-catalyzed oxidations of xenobiotics and procarcinogens by synthetic organoselenium compounds.

1997 • 51 citations

Expression of Cytochrome P450 3A7 inEscherichia coli:Effects of 5′ Modification and Catalytic Characterization of Recombinant Enzyme Expressed in Bicistronic Format with NADPH-Cytochrome P450 Reductase

1997 • 49 citations

2-Methoxyestrone, A Metabolite of Estradiol-17β in the Human¹

1957 • 41 citations

Identification of Retained N-Formylmethionine in Bacterial Recombinant Mammalian Cytochrome P450 Proteins with the N-Terminal Sequence MALLLAVFL...: Roles of Residues 3−5 in Retention and Membrane Topology

1996 • 34 citations

Synthesis of Potential Metabolites of Estradiol¹

1959 • 31 citations

Detergents as probes of reconstituted rat liver cytochrome P-450 function

1987 • 19 citations