Production of Fully Active Recombinant Murine Granzyme B in Yeast

Single-Step Method of RNA Isolation by Acid Guanidinium Thiocyanate–Phenol–Chloroform Extraction

1987 • 64,270 citations

Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction

1987 • 45,673 citations

Apoptosis by Death Factor

1997 • 5,038 citations

Identification and inhibition of the ICE/CED-3 protease necessary for mammalian apoptosis

1995 • 4,003 citations

FLICE, A Novel FADD-Homologous ICE/CED-3–like Protease, Is Recruited to the CD95 (Fas/APO-1) Death-Inducing Signaling Complex

1996 • 3,065 citations

A novel heterodimeric cysteine protease is required for interleukin-1βprocessing in monocytes

1992 • 2,495 citations

Yama/CPP32β, a mammalian homolog of CED-3, is a CrmA-inhibitable protease that cleaves the death substrate poly(ADP-ribose) polymerase

1995 • 2,402 citations

A Combinatorial Approach Defines Specificities of Members of the Caspase Family and Granzyme B

1997 • 2,054 citations

Cytotoxic lymphocytes require granzyme B for the rapid induction of DNA fragmentation and apoptosis in allogeneic target cells

1994 • 844 citations

Involvement of an ICE-like protease in Fas-mediated apoptosis

1995 • 757 citations

Activation of the apoptotic protease CPP32 by cytotoxic T-cell-derived granzyme B

1995 • 720 citations

Requirement of an ICE/CED-3 protease for Fas/APO-1-mediated apoptosis

1995 • 616 citations

Zinc Is a Potent Inhibitor of the Apoptotic Protease, Caspase-3

1997 • 486 citations

Alpha-factor-directed synthesis and secretion of mature foreign proteins in Saccharomyces cerevisiae.

1984 • 477 citations

ICE Family Proteases: Mediators of All Apoptotic Cell Death?

1996 • 440 citations

Lymphocyte-mediated cytotoxicity: Two pathways and multiple effector molecules

1994 • 414 citations

Long-range disruption of gene expression by a selectable marker cassette

1996 • 377 citations

The cell-death machine

1996 • 351 citations

A novel human protease similar to the interleukin-1 beta converting enzyme induces apoptosis in transfected cells.

1995 • 313 citations

Granzymes, a Family of Serine Proteases Released from Granules of Cytolytic T Lymphocytes upon T Cell Receptor Stimulation

1988 • 263 citations

Human and murine cytotoxic T lymphocyte serine proteases: subsite mapping with peptide thioester substrates and inhibition of enzyme activity and cytolysis by isocoumarins

1991 • 255 citations

Advances in the use of Pichia pastoris for high-level gene expression

1995 • 240 citations

Size distribution and general structural features of N‐linked oligosaccharides from the methylotrophic yeast, Pichia pastoris

1989 • 239 citations

Target Cell Lysis by CTL Granule Exocytosis Is Independent of ICE/Ced-3 Family Proteases

1997 • 237 citations

Natural killer and lymphokine-activated killer cells require granzyme B for the rapid induction of apoptosis in susceptible target cells.

1995 • 216 citations

Perforin/granzyme-dependent and independent mechanisms are both important for the development of graft-versus-host disease after murine bone marrow transplantation.

1997 • 212 citations

Generation of active myeloid and lymphoid granule serine proteases requires processing by the granule thiol protease dipeptidyl peptidase I.

1993 • 205 citations

Human cytotoxic lymphocyte granzyme B. Its purification from granules and the characterization of substrate and inhibitor specificity.

1991 • 185 citations

Proteolytic activation of the cell death protease Yama/CPP32 by granzyme B.

1996 • 175 citations

Different Interleukin-1β Converting Enzyme (ICE) Family Protease Requirements for the Apoptotic Death of T Lymphocytes Triggered by Diverse Stimuli

1996 • 164 citations

Methylotrophic yeasts as factories for the production of foreign proteins

1995 • 155 citations

Cleavage of CPP32 by Granzyme B Represents a Critical Role for Granzyme B in the Induction of Target Cell DNA Fragmentation

1996 • 148 citations

The role of granzyme B in murine models of acute graft-versus-host disease and graft rejection

1996 • 138 citations

Expression of recombinant human granzyme B. A processing and activation role for dipeptidyl peptidase I.

1995 • 127 citations

Recombinant human granzyme A binds to two putative HLA-associated proteins and cleaves one of them

1997 • 108 citations

Activation of recombinant murine cytotoxic cell proteinase-1 requires deletion of an amino-terminal dipeptide.

1993 • 81 citations

Conversion of the substrate specificity of mouse proteinase granzyme B

1994 • 74 citations

Expression of Human Recombinant Granzyme A Zymogen and Its Activation by the Cysteine Proteinase Cathepsin C

1996 • 70 citations

The role of granzyme B cluster proteases in cell-mediated cytotoxicity

1997 • 52 citations

Protease Activity of in Vitro Transcribed and Translated Caenorhabditis elegans Cell Death Gene (ced-3) Product

1996 • 44 citations

Processing of procarboxypeptidase A and other zymogens in murine mast cells.

1994 • 43 citations