Molecular Characterization of Ste20p, a Potential Mitogen-activated Protein or Extracellular Signal-regulated Kinase Kinase (MEK) Kinase Kinase from Saccharomyces cerevisiae

Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4

1970 • 253,849 citations

Molecular Cloning: A Laboratory Manual

2001 • 133,517 citations

DNA sequencing with chain-terminating inhibitors

1977 • 69,181 citations

Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.

1979 • 54,907 citations

Molecular Cloning. A Laboratory Manual

1983 • 27,328 citations

Primer-Directed Enzymatic Amplification of DNA with a Thermostable DNA Polymerase

1988 • 17,059 citations

Antibodies, a laboratory manual

1989 • 12,929 citations

Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase

1988 • 6,107 citations

[19] Rapid and efficient site-specific mutagenesis without phenotypic selection

1987 • 5,413 citations

The Protein Kinase Family: Conserved Features and Deduced Phylogeny of the Catalytic Domains

1988 • 5,115 citations

Antibodies: A laboratory manual

1989 • 4,532 citations

ERKs: A family of protein-serine/threonine kinases that are activated and tyrosine phosphorylated in response to insulin and NGF

1991 • 1,901 citations

A brain serine/threonine protein kinase activated by Cdc42 and Rac1

1994 • 1,520 citations

[11] Phosphopeptide mapping and phosphoamino acid analysis by two-dimensional separation on thin-layer cellulose plates

1991 • 1,463 citations

Raf-1 activates MAP kinase-kinase

1992 • 1,308 citations

Signal transduction via the MAP kinases: proceed at your own RSK.

1993 • 1,262 citations

[2] Protein kinase catalytic domain sequence database: Identification of conserved features of primary structure and classification of family members

1991 • 1,225 citations

Requirement for integration of signals from two distinct phosphorylation pathways for activation of MAP kinase

1990 • 1,087 citations

A Divergence in the MAP Kinase Regulatory Network Defined by MEK Kinase and Raf

1993 • 1,086 citations

Formation and Activation of a Cyclin E-cdk2 Complex During the G ₁ Phase of the Human Cell Cycle

1992 • 1,046 citations

Identification of the regulatory phosphorylation sites in pp42/mitogen-activated protein kinase (MAP kinase).

1991 • 1,041 citations

MAP kinase pathways in yeast: For mating and more

1995 • 1,009 citations

The Primary Structure of MEK, a Protein Kinase that Phosphorylates the ERK Gene Product

1992 • 931 citations

Activation of the MAP kinase pathway by the protein kinase raf

1992 • 816 citations

Ras-dependent activation of MAP kinase pathway mediated by G-protein βγ subunits

1994 • 814 citations

Methods in yeast genetics, laboratory manual

1987 • 733 citations

Cell cycle regulation of CDK2 activity by phosphorylation of Thr160 and Tyr15.

1992 • 638 citations

Atomic structure of the MAP kinase ERK2 at 2.3 Å resolution

1994 • 591 citations

Identification of the sites in MAP kinase kinase-1 phosphorylated by p74raf-1.

1994 • 551 citations

Ste5 tethers multiple protein kinases in the MAP kinase cascade required for mating in S. cerevisiae

1994 • 499 citations

The STE4 and STE18 genes of yeast encode potential β and γ subunits of the mating factor receptor-coupled G protein

1989 • 491 citations

cAMP and beta gamma subunits of heterotrimeric G proteins stimulate the mitogen-activated protein kinase pathway in COS-7 cells.

1994 • 473 citations

FUS3 encodes a cdc2+/CDC28-related kinase required for the transition from mitosis into conjugation

1990 • 437 citations

The protein kinase homologue Ste20p is required to link the yeast pheromone response G-protein beta gamma subunits to downstream signalling components.

1992 • 427 citations

Activation of MEK family kinases requires phosphorylation of two conserved Ser/Thr residues.

1994 • 388 citations

Yeast Saccharomyces cerevisiae selectable markers in pUC18 polylinkers

1990 • 376 citations

Rotation of photoreceptor clusters in the developing drosophila eye requires the nemo gene

1994 • 342 citations

Protein-protein interactions in the yeast pheromone response pathway: Ste5p interacts with all members of the MAP kinase cascade.

1994 • 329 citations

MKK1 and MKK2, Which Encode Saccharomyces cerevisiae Mitogen-Activated Protein Kinase-Kinase Homologs, Function in the Pathway Mediated by Protein Kinase C

1993 • 306 citations

A putative protein kinase overcomes pheromone-induced arrest of cell cycling in S. cerevisiae

1989 • 298 citations

Signal transduction in Saccharomyces cerevisiae requires tyrosine and threonine phosphorylation of FUS3 and KSS1.

1992 • 292 citations

Constitutive mutants of the protein kinase STE11 activate the yeast pheromone response pathway in the absence of the G protein.

1992 • 292 citations

A new human p34 protein kinase, CDK2, identified by complementation of a cdc28 mutation in Saccharomyces cerevisiae, is a homolog of Xenopus Eg1.

1991 • 265 citations

Complexes between STE5 and components of the pheromone-responsive mitogen-activated protein kinase module.

1994 • 243 citations

byr2, a Schizosaccharomyces pombe gene encoding a protein kinase capable of partial suppression of the ras1 mutant phenotype.

1991 • 208 citations

MAP kinase-related FUS3 from S. cerevisiae is activated by STE7 in vitro

1993 • 190 citations

A dominant truncation allele identifies a gene, STE20, that encodes a putative protein kinase necessary for mating in Saccharomyces cerevisiae.

1993 • 189 citations

Nucleotide sequence of the yeast regulatory gene STE7 predicts a protein homologous to protein kinases.

1986 • 189 citations

A rapid procedure for preparing fluorescein-labeled specific antibodies from whole antiserum: its use in analyzing cytoskeletal architecture.

1983 • 189 citations

Order of action of components in the yeast pheromone response pathway revealed with a dominant allele of the STE11 kinase and the multiple phosphorylation of the STE7 kinase.

1992 • 188 citations

STE11 is a protein kinase required for cell-type-specific transcription and signal transduction in yeast.

1990 • 178 citations

Pheromone-induced signal transduction in Saccharomyces cerevisiae requires the sequential function of three protein kinases.

1993 • 146 citations

Overexpression of the STE4 gene leads to mating response in haploid Saccharomyces cerevisiae.

1990 • 145 citations

Identification of insulin‐stimulated protein kinase‐1 as the rabbit equivalent of rsk^mo‐2

1993 • 141 citations

Constitutive mutant and putative regulatory serine phosphorylation site of mammalian MAP kinase kinase (MEK1).

1994 • 128 citations

Reconstitution of a yeast protein kinase cascade in vitro: activation of the yeast MEK homologue STE7 by STE11.

1994 • 122 citations

Common Modifications of Trimeric G Proteins and ras Protein: Involvement of Polyisoprenylation

1990 • 122 citations

Dominant-negative mutants of a yeast G-protein beta subunit identify two functional regions involved in pheromone signalling.

1992 • 90 citations

Networking with protein kinases

1993 • 62 citations

Cloning of Saccharomyces cerevisiae STE5 as a suppressor of a Ste20 protein kinase mutant: structural and functional similarity of Ste5 to Farl

1993 • 59 citations

STE50, a novel gene required for activation of conjugation at an early step in mating in Saccharomyces cerevisiae

1992 • 49 citations