Mapping determinants of the substrate selectivities of P450 enzymes by site-directed mutagenesis

High-resolution crystal structure of cytochrome P450cam

1987 • 1,396 citations

The P450 Superfamily: Update on New Sequences, Gene Mapping, and Recommended Nomenclature

1991 • 1,092 citations

Alteration of mouse cytochrome P450coh substrate specificity by mutation of a single amino-acid residue

1989 • 382 citations

Oxidative metabolism of cyclophosphamide: identification of the hepatic monooxygenase catalysts of drug activation.

1989 • 179 citations

Secondary structure prediction of 52 membrane-bound cytochromes P450 shows a strong structural similarity to P450cam

1989 • 143 citations

Site-directed mutageneses of rat liver cytochrome P-450d: catalytic activities toward benzphetamine and 7-ethoxycoumarin

1989 • 101 citations

The CYP2D gene subfamily: analysis of the molecular basis of the debrisoquine 4-hydroxylase deficiency in DA rats

1989 • 99 citations

Molecular basis for a functionally unique cytochrome P450IIB1 variant.

1991 • 93 citations

A molecular model for the enzyme cytochrome P45017α, a major target for the chemotherapy of prostatic cancer

1990 • 85 citations

Identification and location of .alpha.-helices in mammalian cytochromes P450

1989 • 83 citations

Sequence requirements for cytochrome P-450IIB1 catalytic activity

1989 • 80 citations

A predicted three-dimensional structure of human cytochrome P450: implications for substrate specificity

1991 • 78 citations

Point mutations at threonine-301 modify substrate specificity of rabbit liver microsomal cytochromes P-450 (laurate (ω-1)-hydroxylase and testosterone 16α-hydroxylase)

1989 • 72 citations

[2] Modeling of mammalian P450s on basis of P450cam x-ray structure

1991 • 69 citations

Functional and structural polymorphism of rabbit microsomal cytochrome P-450 form 3b.

1982 • 68 citations

Sequence requirements for cytochrome P-450IID1 catalytic activity. A single amino acid change (Ile380 Phe) specifically decreases Vmax of the enzyme for bufuralol but not debrisoquine hydroxylation.

1990 • 63 citations

Structural function of residue-209 in coumarin 7-hydroxylase (P450coh). Enzyme-kinetic studies and site-directed mutagenesis.

1991 • 61 citations

Alteration of high and low spin equilibrium by a single mutation of amino acid 209 in mouse cytochromes P450

1991 • 57 citations

A hypervariable region of P450IIC5 confers progesterone 21-hydroxylase activity to P450IIC1

1991 • 50 citations

The structure of phenobarbital-inducible rat liver cytochrome P-450 isoenzyme PB-4. Production and characterization of site-specific antibodies.

1985 • 46 citations

The importance of threonine‐301 from cytochromes P‐450 (laurate (ω‐1)‐hydroxylase and testosterone 16α‐hydroxylase) in substrate binding as demonstrated by site‐directed mutagenesis

1988 • 44 citations

An inhibitory monoclonal antibody binds in close proximity to a determinant for substrate binding in cytochrome P450IIC5.

1991 • 43 citations

Mutations at the distal and proximal sites of cytochrome P-450d changed regio-specificity of acetanilide hydroxylations

1989 • 42 citations