Pyrosequencing: History, biochemistry and future

DNA sequencing with chain-terminating inhibitors

1977 • 69,181 citations

Detection of specific sequences among DNA fragments separated by gel electrophoresis

1975 • 33,059 citations

Initial sequencing and analysis of the human genome

2001 • 23,520 citations

The Sequence of the Human Genome

2001 • 13,500 citations

A new method for sequencing DNA.

1977 • 7,941 citations

Serial Analysis of Gene Expression

1995 • 3,659 citations

Specific Enzymatic Amplification of DNA In Vitro: The Polymerase Chain Reaction

1986 • 3,400 citations

PNA hybridizes to complementary oligonucleotides obeying the Watson–Crick hydrogen-bonding rules

1993 • 2,034 citations

A Sequencing Method Based on Real-Time Pyrophosphate

1998 • 1,593 citations

Kinetic Proofreading: A New Mechanism for Reducing Errors in Biosynthetic Processes Requiring High Specificity

1974 • 1,532 citations

Mutation of POLG is associated with progressive external ophthalmoplegia characterized by mtDNA deletions

2001 • 812 citations

A Mutation in PRKAG3 Associated with Excess Glycogen Content in Pig Skeletal Muscle

2000 • 724 citations

ESCHERICHIA COLI SINGLE-STRANDED DNA-BINDING PROTEIN: Multiple DNA-Binding Modes and Cooperativities

1994 • 604 citations

Identification and Characterization of CD39/Vascular ATP Diphosphohydrolase

1996 • 566 citations

Cloning of firefly luciferase cDNA and the expression of active luciferase in Escherichia coli.

1985 • 514 citations

Ecto-ATPases: Identities and Functions

1995 • 503 citations

Signalling via ATP in the nervous system

1994 • 441 citations

Single-Nucleotide Polymorphism Analysis by Pyrosequencing

2000 • 439 citations

Modulation of firefly luciferase stability and impact on studies of gene regulation

1991 • 437 citations

The single-stranded DNA-binding protein of Escherichia coli

1990 • 424 citations

The endothelial cell ecto-ADPase responsible for inhibition of platelet function is CD39.

1997 • 411 citations

Kinetic mechanism of DNA polymerase I (Klenow)

1987 • 352 citations

Sequencing of megabase plus DNA by hybridization: Theory of the method

1989 • 352 citations

Genetic and Crystallographic Studies of the 3′,5′-Exonucleolytic Site of DNA Polymerase I

1988 • 344 citations

Spectral emission and quantum yield of firefly bioluminescence

1960 • 340 citations

CD39 Is an Ecto-(Ca2+,Mg2+)-apyrase

1996 • 290 citations

Kinetic mechanism whereby DNA polymerase I (Klenow) replicates DNA with high fidelity

1988 • 278 citations

Determination of Single-Nucleotide Polymorphisms by Real-time Pyrophosphate DNA Sequencing

2000 • 265 citations

A new method of sequencing DNA

1988 • 264 citations

Mutations in the region encoding the von Willebrand factor A domain of matrilin-3 are associated with multiple epiphyseal dysplasia

2001 • 203 citations

Guanosine diphosphatase is required for protein and sphingolipid glycosylation in the Golgi lumen of Saccharomyces cerevisiae

1993 • 183 citations

Fluorescent in situ sequencing on polymerase colonies

2003 • 182 citations

Elementary steps in the DNA polymerase I reaction pathway

1983 • 179 citations

Enzymatic method for continuous monitoring of DNA polymerase activity

1987 • 168 citations

The single-stranded DNA-binding protein of Escherichia coli.

1990 • 163 citations

Classification, identification and subtyping of bacteria based on pyrosequencing and signature matching of 16S rDNA fragments

2002 • 158 citations

Tandemly repeated genes encode nucleoside triphosphate hydrolase isoforms secreted into the parasitophorous vacuole of Toxoplasma gondii.

1994 • 157 citations

Proteolytic Cleavage of Native DNA Polymerase into Two Different Catalytic Fragments

1971 • 147 citations

DNA Sequencing by Mass Spectrometry

1996 • 139 citations

DNA Sequencing up to 1300 Bases in Two Hours by Capillary Electrophoresis with Mixed Replaceable Linear Polyacrylamide Solutions

2000 • 134 citations

Rate-limiting steps in the DNA polymerase I reaction pathway

1985 • 133 citations

DNA replication.

1988 • 130 citations

The nucleotide analog 2-aminopurine as a spectroscopic probe of nucleotide incorporation by the Klenow fragment of Escherichia coli polymerase I and bacteriophage T4 DNA polymerase

1995 • 128 citations

The effect of single base‐pair mismatches on the duplex stability of d(T‐A‐T‐T‐A‐A‐T‐A‐T‐C‐A‐A‐G‐T‐T‐G) · d(C‐A‐A‐C‐T‐T‐G‐A‐T‐A‐T‐T‐A‐A‐T‐A)

1984 • 128 citations

Functional and phenotypic studies of two variants of a human mast cell line with a distinct set of mutations in the c‐kit proto‐oncogene

2003 • 119 citations

Apyrases (ATP diphosphohydrolases, EC 3.6.1.5) : function and relationship to ATPases

1996 • 117 citations

Peptide nucleic acids and their potential applications in biotechnology

1993 • 114 citations

[Determination of the nucleotide sequence of DNA using hybridization with oligonucleotides. A new method].

1988 • 113 citations

Improved thermostability of the North American firefly luciferase: saturation mutagenesis at position 354

1996 • 110 citations

DNA Sequencing by Primer Walking with Strings of Contiguous Hexamers

1992 • 110 citations

Luciferase from the East European firefly Luciola mingrelica: Cloning and nucleotide sequence of the cDNA, overexpression in Escherichia coli and purification of the enzyme

1993 • 110 citations

Firefly Luciferase

1976 • 105 citations

Mutation detection by pyrosequencing: sequencing of exons 5–8 of the p53 tumor suppressor gene

2000 • 100 citations

Typing of Human Papillomavirus by Pyrosequencing

2001 • 94 citations

Improved Performance of Pyrosequencing Using Single-Stranded DNA-Binding Protein

2000 • 92 citations

ATP Sulfurylase from Higher Plants: Kinetic and Structural Characterization of the Chloroplast and Cytosol Enzymes from Spinach Leaf

1993 • 88 citations

Amplification of bacterial DNA using highly conserved sequences: automated analysis and potential for molecular triage of sepsis.

1995 • 87 citations

Mitochondrial Sequence Analysis for Forensic Identification Using Pyrosequencing Technology

2002 • 85 citations

Thermostabilization of firefly luciferase by a single amino acid substitution at position 217

1993 • 83 citations

The production of oxyluciferin during the firefly luciferase light reaction

1975 • 81 citations

Purification of rat liver N-heparan-sulfate sulfotransferase.

1988 • 78 citations

Micromachined filter-chamber array with passive valves for biochemical assays on beads

2001 • 77 citations

Rapid molecular identification and subtyping of Helicobacter pylori by pyrosequencing of the 16S rDNA variable V1 and V3 regions

2001 • 77 citations

Sequence and biochemical similarities between the luciferases of the glow-worm Lampyris noctiluca and the firefly Photinus pyralis

1996 • 76 citations

Production of Adenosine Triphosphate in Normal Cells and Sporulation Mutants of Bacillus subtilis

1969 • 76 citations

Molecular cloning and expression in Escherichia coli of a cDNA clone encoding luciferase of a firefly, Luciola lateralis

1992 • 74 citations

Multiplex Pyrosequencing

2002 • 74 citations

Pyrosequencing for Detection of Lamivudine-Resistant Hepatitis B Virus

2004 • 74 citations

A photocleavable fluorescent nucleotide for DNA sequencing and analysis

2003 • 67 citations

Sulfate-activating enzymes

1987 • 64 citations

Monitoring Resistance to Human Immunodeficiency Virus Type 1 Protease Inhibitors by Pyrosequencing

2001 • 63 citations

Both risk alleles for FcγRIIA and FcγRIIIA are susceptibility factors for SLE: a unifying hypothesis

2004 • 58 citations

Does 77→G in PTPRC modify autoimmune disorders linked to the major histocompatibility locus?

2001 • 57 citations

Genotyping by apyrase-mediated allele-specific extension

2001 • 57 citations

Analysis of the p53 Tumor Suppressor Gene by Pyrosequencing

2000 • 57 citations

Epidermal Langerhans Cells Are Resistant to the Permeabilizing Effects of Extracellular ATP: In Vitro Evidence Supporting a Protective Role of Membrane ATPase

1993 • 55 citations

Minimizing deletion mutagenesis artifact duringTaqDNA polymerase PCR byE.coliSSB

1992 • 54 citations

Luciferase cDNA from Japanese firefly, Luciola cruciata: Cloning, structure and expression in Escherichia coli

1989 • 53 citations

Bioluminescent Immunoassay with a Protein A-Luciferase Fusion Protein

1993 • 50 citations

Determination of hepatitis C virus genotype by Pyrosequencing

2003 • 48 citations

Cloning, Overexpression, and Purification of the Recombinant His-Tagged SSB Protein ofEscherichia coliand Use in Polymerase Chain Reaction Amplification

1999 • 47 citations

Polymorphisms of the Fcγ receptor type IIB gene are not associated with systemic lupus erythematosus in the Swedish population

2004 • 47 citations

Fractionation of Extracts of Firefly Tails by Gel Filtration.

1968 • 46 citations

PNA and LNA throw light on DNA

2002 • 44 citations

Production, Purification, and Luminometric Analysis of RecombinantSaccharomyces cerevisiae MET3Adenosine Triphosphate Sulfurylase Expressed inEscherichia coli

1999 • 43 citations

Pyrosequencing as an Alternative to Single-Strand Conformation Polymorphism Analysis for Detection of N-ras Mutations in Human Melanoma Metastases

2002 • 42 citations

Automated magnetic preparation of DNA templates for solid phase sequencing

1992 • 41 citations

[1] Cloning firefly luciferase

1986 • 40 citations

Pyrosequencing as a Method for Grouping of Listeria monocytogenes Strains on the Basis of Single-Nucleotide Polymorphisms in the inlB Gene

2001 • 39 citations

Multiple‐primer DNA sequencing method

2003 • 38 citations

Method Enabling Fast Partial Sequencing of cDNA Clones

2001 • 34 citations

The effect of detergents on firefly luciferase reactions

1991 • 33 citations

Preparation of a genetically fused protein A/luciferase conjugate for use in bioluminescent immunoassays

1991 • 31 citations

A convenient affinity chromatography-based purification of firefly luciferase

1980 • 28 citations

Separation of firefly luciferase using an anion exchanger

1976 • 27 citations

Integrated strategy for selective expanded bed ion-exchange adsorption and site-specific protein processing using gene fusion technology

2002 • 27 citations

HPV-related Cancer Susceptibility and p53 Codon 72 Polymorphism

2001 • 27 citations

Applications of mass spectrometry to DNA sequencing

1991 • 25 citations

Multiple group-specific sequencing primers for reliable and rapid DNA sequencing

2003 • 25 citations

Gene expression analysis by signature pyrosequencing

2002 • 23 citations

Pyrosequencing Technology as a Method for the Diagnosis of Multiple Endocrine Neoplasia Type 2

2004 • 22 citations

Identification and subcellular localization of two isoenzymes of apyrase from Solanum tuberosum

1992 • 21 citations

Purification and characterization of two isoapyrases from Solanum tuberosum var. ultimus

1992 • 19 citations

Molecular Haplotyping by PyrosequencingTM

2002 • 18 citations

New approaches to the preparation and application of firefly luciferase

1989 • 18 citations

SNP Analysis by Allele-Specific Extension in a Micromachined Filter Chamber

2002 • 18 citations

Quantification of HIV-1 Using Multiple Quantitative Polymerase Chain Reaction Standards and Bioluminometric Detection

2001 • 18 citations

Transcriptional Regulation of the Yeast DnaJ Homologue SIS1

1996 • 18 citations

SCREENING AND SCANNING OF SINGLE NUCLEOTIDE POLYMORPHISMS IN THE PIG MELANOCORTIN 1 RECEPTOR GENE (MC1R) BY PYROSEQUENCING

2001 • 15 citations

Single-stranded DNA binding protein facilitates amplification of genomic sequences by PCR.

1992 • 14 citations

Incorporation of single-stranded DNA binding protein early in polymerase chain reaction product sequencing reactions prevents enzyme pausing

1992 • 13 citations

Large-scale genomic sequencing: optimization of genomic chemical sequencing reactions.

1995 • 6 citations

Fluorescence studies of ATP-diphosphohydrolase from Solanum tuberosum var. Desirée

2000 • 5 citations

Expression of recombinant firefly luciferase in prokaryotic and eukaryotic cells

1992 • 2 citations