Secretion in yeast: Reconstitution of the translocation and glycosylation of α-factor and invertase in a homologous cell-free system

Transfer of proteins across membranes. I. Presence of proteolytically processed and unprocessed nascent immunoglobulin light chains on membrane-bound ribosomes of murine myeloma.

1975 • 3,026 citations

Functional messenger RNAs are produced by SP6in vitrotranscription of cloned cDNAs

1984 • 1,533 citations

Transfer of proteins across membranes. II. Reconstitution of functional rough microsomes from heterologous components.

1975 • 1,175 citations

Import of proteins into mitochondria. Cytochrome b2 and cytochrome c peroxidase are located in the intermembrane space of yeast mitochondria.

1982 • 1,152 citations

Purification and Properties of an Endo-β-N-acetylglucosaminidase from Streptomyces griseus

1974 • 870 citations

Isolation of the putative structural gene for the lysine-arginine-cleaving endopeptidase required for processing of yeast prepro-α-factor

1984 • 698 citations

Secretory protein translocation across membranes—the role of the ‘docking protein’

1982 • 648 citations

Translocation of proteins across the endoplasmic reticulum. I. Signal recognition protein (SRP) binds to in-vitro-assembled polysomes synthesizing secretory protein.

1981 • 644 citations

Structure of a yeast pheromone gene (MFα): A putative α-factor precursor contains four tandem copies of mature α-factor

1982 • 587 citations

IMPORT OF PROTEINS INTO MITOCHONDRIA

1991 • 583 citations

Construction of a new family of high efficiency bacterial expression vectors: identification of cDNA clones coding for human liver proteins.

1984 • 538 citations

Synchronised transmembrane insertion and glycosylation of a nascent membrane protein

1977 • 529 citations

Purification of a membrane-associated protein complex required for protein translocation across the endoplasmic reticulum.

1980 • 502 citations

Protein translocation across the endoplasmic reticulum. II. Isolation and characterization of the signal recognition particle receptor.

1982 • 478 citations

Glycosylation and processing of prepro-α-factor through the yeast secretory pathway

1984 • 410 citations

Yeast α factor is processed from a larger precursor polypeptide: The essential role of a membrane-bound dipeptidyl aminopeptidase

1983 • 403 citations

[8] Immunoprecipitation of proteins from cell-free translations

1983 • 302 citations

Cell-free synthesis and membrane insertion of mouse H-2Dd histocompatibility antigen and β2-microglobulin

1979 • 274 citations

Post-translational cleavage of presecretory proteins with an extract of rough microsomes from dog pancreas containing signal peptidase activity.

1977 • 259 citations

A novel in vitro transcription-translation system: accurate and efficient synthesis of single proteins from cloned DNA sequences.

1984 • 251 citations

A SIMPLE METHOD FOR PRODUCING ANTIBODY SPECIFIC TO A SINGLE SELECTED DIFFUSIBLE ANTIGEN

1966 • 230 citations

Nucleotide sequence of The yeastSUC2gene for invertase

1983 • 225 citations

Efficient cleavage and segregation of nascent presecretory proteins in a reticulocyte lysate supplemented with microsomal membranes.

1978 • 213 citations

Assembly of the semliki forest virus membrane glycoproteins in the membrane of the endoplasmic reticulum in vitro

1978 • 178 citations

Invertase signal and mature sequence substitutions that delay intercompartmental transport of active enzyme.

1985 • 167 citations

The secreted form of invertase in Saccharomyces cerevisiae is synthesized from mRNA encoding a signal sequence.

1983 • 164 citations

Secretion of Human Interferons by Yeast

2019 • 152 citations

The synthesis and in vivo assembly of functional antibodies in yeast

1985 • 145 citations

Distinct repressible mRNAs for cytoplasmic and secreted yeast invertase are encoded by a single gene

1981 • 144 citations

A membrane component essential for vectorial translocation of nascent proteins across the endoplasmic reticulum: requirements for its extraction and reassociation with the membrane.

1980 • 140 citations

Yeast secretory mutants that block the formation of active cell surface enzymes.

1984 • 131 citations

Secretion of a wheat α-amylase expressed in yeast

1984 • 120 citations

Presecretory and cytoplasmic invertase polypeptides encoded by distinct mRNAs derived from the same structural gene differ by a signal sequence.

1982 • 120 citations

The preparation and characterization of a cell-free system from Saccharomyces cerevisiae that translates natural messenger ribonucleic acid.

1979 • 115 citations

Protein transfer across microsomal membranes reassembled from separated membrane components

1978 • 114 citations

Hormone processing and membrane-bound proteinases in yeast.

1985 • 114 citations

Mechanism of compartmentation of secretory proteins: transport of exocrine pancreatic proteins across the microsomal membrane

1980 • 99 citations

Proteolysis in eukaryotic cells. Identification of multiple proteolytic enzymes in yeast.

1984 • 95 citations

Expression and processing of bacterial beta-lactamase in the yeast Saccharomyces cerevisiae.

1981 • 87 citations

Genes required for completion of import of proteins into the endoplasmic reticulum in yeast.

1984 • 86 citations

Faithful and efficient translation of homologous and heterologous mRNAs in an mRNA-dependent cell-free system from Saccharomyces cerevisiae.

1980 • 74 citations

Characterization of a gene product (Sec53p) required for protein assembly in the yeast endoplasmic reticulum.

1985 • 69 citations

Functional interaction of plant ribosomes with animal microsomal membranes

1977 • 69 citations

A structural and functional analysis of the docking protein. Characterization of active domains by proteolysis and specific antibodies.

1985 • 61 citations

Precursor of beta-lactamase is enzymatically inactive. Accumulation of the preprotein in Saccharomyces cerevisiae.

1985 • 47 citations

Processing in vitro of placental peptide hormones by smooth microsomes.

1979 • 40 citations

Segregation of the polypeptide translocation apparatus to regions of the endoplasmic reticulum containing ribophorins and ribosomes. I. Functional tests on rat liver microsomal subfractions.

1984 • 40 citations

POST‐TRANSLATIONAL UPTAKE OF CYTOPLASMICALLY SYNTHESIZED PROTEINS BY INTACT CHLOROPLASTS IN VITRO*

1980 • 39 citations

Yeast pheromone α-factor is synthesized as a high molecular weight precursor

1983 • 34 citations

Specific processing of the bacterial ?-lactamase precursor in Saccharomyces cerevisiae

1983 • 14 citations

Isolation and characterisation of rough and smooth endoplasmic reticulum fromSaccharomyces cerevisiae

1982 • 13 citations

In vitro synthesis of biologically active human leukocyte interferon in a RNA-dependent system from Saccharomyces cerevisiae

1983 • 8 citations

Deleted Work

1955 • 0 citations

Cited By (0)

No citing papers found in database