Expression of mutant ELH prohormones in AtT-20 cells: the relationship between prohormone processing and sorting.

DNA sequencing with chain-terminating inhibitors

1977 • 69,181 citations

Single-Step Method of RNA Isolation by Acid Guanidinium Thiocyanate–Phenol–Chloroform Extraction

1987 • 64,270 citations

Rapid and efficient site-specific mutagenesis without phenotypic selection.

1985 • 7,573 citations

The trans Golgi Network: Sorting at the Exit Site of the Golgi Complex

1986 • 1,175 citations

Pathways of Protein Secretion in Eukaryotes

1985 • 1,050 citations

Calcium phosphate-mediated gene transfer: a highly efficient transfection system for stably transforming cells with plasmid DNA.

1988 • 897 citations

Protein translocation across the endoplasmic reticulum

1984 • 825 citations

Mammalian subtilisins: The long-sought dibasic processing endoproteases

1991 • 756 citations

Endoproteolytic cleavage of gp160 is required for the activation of human immunodeficiency virus

1988 • 608 citations

Post-Translational Proteolysis in Polypeptide Hormone Biosynthesis

1982 • 590 citations

Arg-X-Lys/Arg-Arg motif as a signal for precursor cleavage catalyzed by furin within the constitutive secretory pathway

1991 • 590 citations

PC1 and PC2 are proprotein convertases capable of cleaving proopiomelanocortin at distinct pairs of basic residues.

1991 • 589 citations

Identification of a human insulinoma cDNA encoding a novel mammalian protein structurally related to the yeast dibasic processing protease Kex2.

1990 • 579 citations

Pre- and post-golgi vacuoles operate in the transport of semliki forest virus membrane glycoproteins to the cell surface

1984 • 523 citations

POLYPROTEIN GENE EXPRESSION: Generation of Diversity of Neuroendocrine Peptides

1984 • 497 citations

Cloning and Primary Sequence of a Mouse Candidate Prohormone Convertase PC1 Homologous to PC2, Furin, and Kex2: Distinct Chromosomal Localization and Messenger RNA Distribution in Brain and Pituitary Compared to PC2

1991 • 475 citations

Identification of a cDNA encoding a second putative prohormone convertase related to PC2 in AtT20 cells and islets of Langerhans.

1991 • 449 citations

Intracellular Targeting and Structural Conservation of a Prohormone-Processing Endoprotease

1989 • 395 citations

Human fur gene encodes a yeast KEX2-like endoprotease that cleaves pro-beta-NGF in vivo.

1990 • 378 citations

Expressing a human proinsulin cDNA in a mouse ACTH-secreting cell. Intracellular storage, proteolytic processing, and secretion on stimulation

1983 • 329 citations

Kex2-like endoproteases PC2 and PC3 accurately cleave a model prohormone in mammalian cells: evidence for a common core of neuroendocrine processing enzymes.

1991 • 317 citations

Direct identification of prohormone conversion site in insulin-secreting cells

1985 • 297 citations

The trans-most cisternae of the Golgi complex: A compartment for sorting of secretory and plasma membrane proteins

1987 • 275 citations

Proteolysis in Neuropeptide Processing and Other Neural Functions

1984 • 264 citations

Furin is a subtilisin-like proprotein processing enzyme in higher eukaryotes

1990 • 259 citations

Tyrosine Sulfation and the Secretory Pathway

1988 • 257 citations

Perturbation of the morphology of the trans-Golgi network following Brefeldin A treatment: redistribution of a TGN-specific integral membrane protein, TGN38.

1992 • 226 citations

Compartmentation of the Golgi complex: brefeldin-A distinguishes trans-Golgi cisternae from the trans-Golgi network.

1990 • 222 citations

Multiple neuropeptides derived from a common precursor are differentially packaged and transported

1988 • 196 citations

Precursors for peptide hormones share common secondary structures forming features at the proteolytic processing sites

1986 • 183 citations

Defective propeptide processing of blood clotting factor IX caused by mutation of arginine to glutamine at position −4

1986 • 176 citations

Dale's hypothesis revisited: different neuropeptides derived from a common prohormone are targeted to different processes.

1990 • 159 citations

fur gene expression as a discriminating marker for small cell and nonsmall cell lung carcinomas.

1987 • 144 citations

Re-routing of a secretory protein by fusion with human growth hormone sequences

1986 • 138 citations

Sorting within the regulated secretory pathway occurs in the trans-Golgi network.

1990 • 127 citations

Sequence requirements for precursor cleavage within the constitutive secretory pathway.

1992 • 113 citations

Peptide Processing and Targeting in the Neuronal Secretory Pathway

1991 • 107 citations

Phosphorylation of pro-adrenocorticotropin/endorphin-derived peptides.

1982 • 79 citations

Structure and expression of the egg-laying hormone gene family in Aplysia

1985 • 72 citations

Localization of Aplysia neurosecretory peptides to multiple populations of dense core vesicles.

1986 • 72 citations

Processing of the egg-laying hormone (ELH) precursor in the bag cell neurons of Aplysia.

1988 • 63 citations

Effects of amino acid replacements within the tetrabasic cleavage site on the processing of the human insulin receptor precursor expressed in Chinese hamster ovary cells.

1990 • 62 citations

Prohormone processing in Xenopus oocytes: characterization of cleavage signals and cleavage enzymes.

1991 • 52 citations

Evidence for the presence of a secondary structure at the dibasic processing site of prohormone: the pro-ocytocin model.

1992 • 49 citations

Rapid and efficient site-specific mutagenesis

1990 • 45 citations

Prohormone convertases revealed at last

1991 • 35 citations