Oligodeoxynucleotides containing CpG motifs induce IL-12, IL-18 and IFN-γ production in cells from allergic individuals and inhibit IgE synthesisin vitro

Single-Step Method of RNA Isolation by Acid Guanidinium Thiocyanate–Phenol–Chloroform Extraction

1987 • 64,270 citations

Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction

1987 • 45,673 citations

Efficient presentation of soluble antigen by cultured human dendritic cells is maintained by granulocyte/macrophage colony-stimulating factor plus interleukin 4 and downregulated by tumor necrosis factor alpha.

1994 • 5,204 citations

CpG motifs in bacterial DNA trigger direct B-cell activation

1995 • 3,543 citations

Cloning of a new cytokine that induces IFN-γ production by T cells

1995 • 2,584 citations

Proliferating dendritic cell progenitors in human blood.

1994 • 1,922 citations

CpG motifs present in bacteria DNA rapidly induce lymphocytes to secrete interleukin 6, interleukin 12, and interferon gamma.

1996 • 1,498 citations

PROPHYLACTIC INOCULATION AGAINST HAY FEVER

1911 • 1,317 citations

CpG Oligodeoxynucleotides Act as Adjuvants that Switch on T Helper 1 (Th1) Immunity

1997 • 1,008 citations

Role of interleukin 10 in specific immunotherapy.

1998 • 980 citations

Immunostimulatory DNA sequences function as T helper-1-promoting adjuvants

1997 • 919 citations

Bacterial DNA and immunostimulatory CpG oligonucleotides trigger maturation and activation of murine dendritic cells

1998 • 809 citations

Evidence for compartmentalization of functional subsets of CD2+ T lymphocytes in atopic patients.

1990 • 807 citations

Induction of NK activity in murine and human cells by CpG motifs in oligodeoxynucleotides and bacterial DNA.

1996 • 764 citations

Macrophages ingest and are activated by bacterial DNA.

1996 • 579 citations

Modulation of airway inflammation by CpG oligodeoxynucleotides in a murine model of asthma.

1998 • 557 citations

Allergen immunotherapy decreases interleukin 4 production in CD4+ T cells from allergic individuals.

1993 • 549 citations

Bee venom immunotherapy results in decrease of IL-4 and IL-5 and increase of IFN-gamma secretion in specific allergen-stimulated T cell cultures.

1995 • 533 citations

Preferential induction of a Th1 immune response and inhibition of specific IgE antibody formation by plasmid DNA immunization.

1996 • 524 citations

Transforming Growth Factor β1, in the Presence of Granulocyte/Macrophage Colony-stimulating Factor and Interleukin 4, Induces Differentiation of Human Peripheral Blood Monocytes into Dendritic Langerhans Cells

1998 • 507 citations

Unique palindromic sequences in synthetic oligonucleotides are required to induce IFN [correction of INF] and augment IFN-mediated [correction of INF] natural killer activity.

1992 • 480 citations

Macrophages sense pathogens via DNA motifs: induction of tumor necrosis factor‐α‐mediated shock

1997 • 422 citations

Grass pollen immunotherapy inhibits allergen-induced infiltration of CD4+ T lymphocytes and eosinophils in the nasal mucosa and increases the number of cells expressing messenger RNA for interferon-γ

1996 • 420 citations

Cutting Edge: Modulation of Airway Inflammation by CpG Oligodeoxynucleotides in a Murine Model of Asthma

1998 • 416 citations

CpG‐containing synthetic oligonucleotides promote B and cytotoxic T cell responses to protein antigen: A new class of vaccine adjuvants

1997 • 390 citations

Usefulness of immunotherapy in patients with severe summer hay fever uncontrolled by antiallergic drugs.

1991 • 390 citations

Immunological changes during specific immunotherapy of grass pollen allergy: reduced lymphoproliferative responses to allergen and shift from TH₂ to TH₁ in T‐cell clones specific for Phi p 1, a major grass pollen allergen

1997 • 331 citations

Prevalence of Atopy and Pollinosis in the Adult Population of Switzerland (SAPALDIA Study)

1995 • 316 citations

Dissection of immunoglobulin E and T lymphocyte reactivity of isoforms of the major birch pollen allergen Bet v 1: potential use of hypoallergenic isoforms for immunotherapy.

1996 • 315 citations

CpG motifs as immune adjuvants

1999 • 313 citations

Regulation of IgE Synthesis

1984 • 301 citations

Bacterial DNA-Induced NK Cell IFN-γ Production Is Dependent on Macrophage Secretion of IL-12

1997 • 300 citations

Immunoprophylaxis of allergen–induced immunoglobulin E synthesis and airway hyperresponsiveness in vivo by genetic immunization

1996 • 293 citations

Activation of human B cells by phosphorothioate oligodeoxynucleotides.

1996 • 292 citations

Regulation of the development of type 2 T-helper cells in allergy

1994 • 276 citations

Modulation of IgE reactivity of allergens by site‐directed mutagenesis: potential use of hypoallergenic variants for immunotherapy

1998 • 271 citations

Interleukin 4 production by CD4+ T cells from allergic individuals is modulated by antigen concentration and antigen-presenting cell type.

1995 • 271 citations

Effect of immunotherapy on immunoglobulin E and immunoglobulin G antibodies to ragweed antigens: A six-year prospective study

1982 • 257 citations

Immunostimulatory DNA: Sequence‐dependent production of potentially harmful or useful cytokines

1997 • 248 citations

The Subclass Nature and Clinical Significance of the IgG Antibody Response in Patients Undergoing Allergen‐Specific Immunotherapy

1985 • 210 citations

IFN-gamma promotes IL-6 and IgM secretion in response to CpG motifs in bacterial DNA and oligodeoxynucleotides.

1996 • 208 citations

DNA as an Adjuvant: Capacity of Insect DNA and Synthetic Oligodeoxynucleotides to Augment T Cell Responses to Specific Antigen

1998 • 159 citations

Differences in clinical and immunologic reactivity of patients allergic to grass pollens and to multiple-pollen species

1991 • 150 citations

IgG Subclass Antibody Response in Grass Pollen‐Allergic Patients Undergoing Specific Immunotherapy

1984 • 117 citations

Oligonucleotide Adjuvants for T Helper 1 (Th1)–specific Vaccination

1997 • 109 citations

Allergen-induced Cytokine Production in Atopic Disease and Its Relationship to Disease Severity

1997 • 106 citations

Monoclonal Antibodies against Birch Pollen Allergens: Characterization by Immunoblotting and Use for Single-Step Affinity Purification of the Major Allergen <i>Bet </i><i>v</i> I

1989 • 97 citations

Immune responses after immunization with plasmid DNA encoding Bet v 1, the major allergen of birch pollen

1999 • 78 citations

Evaluation of immunotherapy‐induced changes in specific IgE, IgG and IgG subclasses in birch pollen allergic patients by means of immunoblotting

1990 • 70 citations

IL-12 expression in AIDS-related lymphoma B cell lines.

1996 • 56 citations

Effects of adjuvants on the immune response to allergens in a murine model of allergen inhalation: cholera toxin induces a Th1-like response to Bet v 1, the major birch pollen allergen

1998 • 53 citations

Long-Lived Th2 Clones Specific for Seasonal and Perennial Allergens Can Be Detected in Blood and Skin by Their TCR-Hypervariable Regions

1998 • 51 citations

Bacterial DNA as an evolutionary conserved ligand signalling danger of infection to immune cells

1998 • 48 citations

Serum IgE level is negatively correlated with the ability of peripheral mononuclear cells to produce interferon gamma (IFNγ): evidence of reduced expression of IFNγ mRNA in atopic patients

1998 • 45 citations

Bet v 1, the major birch pollen allergen, conjugated to crystalline bacterial cell surface proteins, expands allergen-specific T cells of the Th1/Th0 phenotype in vitro by induction of IL-12

1997 • 34 citations

Allergen-specific IgE production of committed B cells from allergic patients in vitro

1995 • 34 citations

DNA Immunotherapeutics: New Potential Treatment Modalities for Allergic Disease

1998 • 25 citations

THE ASSAY OF INTERLEUKIN 4 IN THE SERUM OF NORMAL SUBJECTS AND ATOPIC PATIENTS USING A NOVEL IMMUNOASSAY

1997 • 8 citations