Continuously recording fluorescent assays optimized for five human matrix metalloproteinases

Measurement of protein using bicinchoninic acid

1985 • 17,516 citations

On the size of the active site in proteases. I. Papain

1967 • 5,150 citations

SV40-transformed Human Lung Fibroblasts Secrete a 92-kDa Type IV Collagenase Which Is Identical to That Secreted by Normal Human Macrophages

1989 • 1,122 citations

H-ras oncogene-transformed human bronchial epithelial cells (TBE-1) secrete a single metalloprotease capable of degrading basement membrane collagen.

1988 • 1,050 citations

A cleavage method which minimizes side reactions following Fmoc solid phase peptide synthesis

1990 • 710 citations

Human fibroblast collagenase. Complete primary structure and homology to an oncogene transformation-induced rat protein.

1986 • 645 citations

A metalloproteinase from human rheumatoid synovial fibroblasts that digests connective tissue matrix components. Purification and characterization.

1986 • 515 citations

Stromelysin, a connective tissue-degrading metalloendopeptidase secreted by stimulated rabbit synovial fibroblasts in parallel with collagenase. Biosynthesis, isolation, characterization, and substrates.

1985 • 506 citations

Comparison of human stromelysin and collagenase by cloning and sequence analysis

1986 • 443 citations

Matrix metalloproteinase 2 from human rheumatoid synovial fibroblasts

1990 • 428 citations

Human neutrophil collagenase. A distinct gene product with homology to other matrix metalloproteinases.

1990 • 406 citations

Human skin collagenase: isolation of precursor and active forms from both fibroblast and organ cultures

1977 • 387 citations

Purification and characterization of a murine basement membrane collagen-degrading enzyme secreted by metastatic tumor cells.

1983 • 245 citations

Expression of a metalloproteinase that degrades native type V collagen and denatured collagens by cultured human alveolar macrophages.

1987 • 235 citations

The complete primary structure of human matrix metalloproteinase-3. Identity with stromelysin.

1988 • 227 citations

Mr 92,000 gelatinase release correlates with the metastatic phenotype in transformed rat embryo cells.

1990 • 202 citations

Matrix metalloproteinases. A review.

1990 • 187 citations

Comparison of vertebrate collagenase and gelatinase using a new fluorogenic substrate peptide

1989 • 154 citations

Cleavage of type VII collagen by interstitial collagenase and type IV collagenase (gelatinase) derived from human skin

1989 • 151 citations

Synthetic substrates for vertebrate collagenase

1977 • 142 citations

Differential susceptibility of type X collagen to cleavage by two mammalian interstitial collagenases and 72-kDa type IV collagenase.

1990 • 121 citations

Sequence specificity of human skin fibroblast collagenase. Evidence for the role of collagen structure in determining the collagenase cleavage site.

1987 • 120 citations

Evidence that human rheumatoid synovial matrix metalloproteinase 3 is an endogenous activator of procollagenase

1988 • 120 citations

Numerical values of the absorbances of the aromatic amino acids in acid, neutral, and alkaline solutions

1968 • 112 citations

Type X collagen contains two cleavage sites for a vertebrate collagenase.

1986 • 106 citations

Degradation of type IX collagen by matrix metalloproteinase 3 (stromelysin) from human rheumatoid synovial cells

1989 • 98 citations

Tumor-promoting phorbol esters and cell proliferation stimulate secretion of basement membrane (type IV) collagen-degrading metalloproteinase by human fibroblasts.

1985 • 96 citations

Characterization of 58-kilodalton human neutrophil collagenase: comparison with human fibroblast collagenase

1990 • 93 citations

Spectrophotometric assay for vertebrate collagenase

1985 • 91 citations

Synthetic substrates of vertebrate collagenase

1985 • 84 citations

Human neutrophil gelatinase is a component of specific granules.

1989 • 77 citations

Susceptibility of cartilage collagens type II, IX, X, and XI to human synovial collagenase and neutrophil elastase

1988 • 73 citations

Fluorescence determination of carboxypeptidase A activity based on electronic energy transfer

1972 • 61 citations

Cleavage specificity of type IV collagenase (gelatinase) from human skin

1989 • 47 citations

Proteolytic activities of human fibroblast collagenase: hydrolysis of a broad range of substrates at a single active site

1990 • 44 citations

Purification to homogeneity of latent and active 58-kilodalton forms of human neutrophil collagenase

1990 • 43 citations

Clostridium histolyticum collagenase: development of new thio ester, fluorogenic, and depsipeptide substrates and new inhibitors

1985 • 36 citations

Amino acid sequence of chick skin collagen .alpha.1(I)-CB8 and the complete primary structure of the helical portion of the chick skin collagen .alpha.1(I) chain

1982 • 35 citations

Substrate specificity of human fibroblast stromelysin. Hydrolysis of substance P and its analogs

1989 • 34 citations

Cleavage of Collagen Type X by Human Synovial Collagenase and Neutrophil Elastase

1989 • 29 citations

A fluorescent oligopeptide energy transfer assay with broad applications for neutral proteases

1989 • 29 citations