Oligomerization domain-directed reassembly of active dihydrofolate reductase from rationally designed fragments

A novel genetic system to detect protein–protein interactions

1989 • 6,022 citations

Database of homology‐derived protein structures and the structural meaning of sequence alignment

1991 • 1,641 citations

A bacterial two-hybrid system based on a reconstituted signal transduction pathway

1998 • 1,560 citations

Structure of the FKBP12-Rapamycin Complex Interacting with Binding Domain of Human FRAP

1996 • 881 citations

Split ubiquitin as a sensor of protein interactions in vivo.

1994 • 772 citations

Loop and Subdomain Movements in the Mechanism of Escherichia coli Dihydrofolate Reductase: Crystallographic Evidence^,

1997 • 650 citations

The 2.2 Å crystal structure of the Ras-binding domain of the serine/threonine kinase c-Raf1 in complex with RaplA and a GTP analogue

1995 • 627 citations

The Phosphorylation State of Eucaryotic Initiation Factor 2 Alters Translational Efficiency of Specific mRNAs

1989 • 393 citations

Directed evolution of enzyme catalysts

1997 • 370 citations

Role of GTP Hydrolysis in Fission of Caveolae Directly from Plasma Membranes

1996 • 370 citations

Monitoring protein–protein interactions in intact eukaryotic cells by β-galactosidase complementation

1997 • 324 citations

Characterization by in vitro complementation of a peptide corresponding to an operator-proximal segment of the β-galactosidase structural gene of Escherichia coli

1967 • 323 citations

Crystal structure of human dihydrofolate reductase complexed with folate

1988 • 219 citations

Crystal structure of unliganded Escherichia coli dihydrofolate reductase. Ligand-induced conformational changes and cooperativity in binding

1991 • 184 citations

Molecular evolution of an arsenate detoxification pathway by DNA shuffling

1997 • 182 citations

Kinetics of the formation and isomerization of methotrexate complexes of recombinant human dihydrofolate reductase.

1988 • 120 citations

Simultaneous Formation of Two Alternative Enzymically Active Structures by Complementation of Two Overlapping Fragments of Staphylococcal Nuclease

1971 • 107 citations

Functional assembly of a randomly cleaved protein.

1992 • 96 citations

Ordered Self-Assembly of Polypeptide Fragments to form Nativelike Dimeric trp Repressor

1992 • 93 citations

Site-directed mutagenesis of mouse dihydrofolate reductase. Mutants with increased resistance to methotrexate and trimethoprim.

1988 • 86 citations

A fully active variant of dihydrofolate reductase with a circularly permuted sequence

1992 • 75 citations

Methotrexate-resistant variants of human dihydrofolate reductase. Effects of Phe31 substitutions

1994 • 65 citations

Probing minimal independent folding units in dihydrofolate reductase by molecular dissection

1997 • 55 citations

Complementation of peptide fragments of the single domain protein chymotrypsin inhibitor 2

1997 • 40 citations

Circularly permuted dihydrofolate reductase of E.coli has functional activity and a destabilized tertiary structure

1994 • 39 citations

Assembly of an active enzyme by the linkage of two protein modules

1997 • 34 citations

Critical Role of Phenylalanine 34 of Human Dihydrofolate Reductase in Substrate and Inhibitor Binding and in Catalysis

1994 • 34 citations

Complementation of Fragments of Triosephosphate Isomerase Defined by Exon Boundaries

1995 • 33 citations

Crystal structure determination at 2.3 A of recombinant human dihydrofolate reductase ternary complex with NADPH and methotrexate-gamma-tetrazole.

1992 • 29 citations

Generation of a Family of Protein Fragments for Structure-folding Studies. 2. Kinetics of Association of the Two Chymotrypsin Inhibitor-2 Fragments

1994 • 26 citations

Dominant negative inhibition by fragments of a monomeric enzyme

1996 • 19 citations

Amino acid replacements can selectively affect the interaction energy of autonomous folding units in the .alpha. subunit of tryptophan synthase

1992 • 13 citations