Kinetics of the formation and isomerization of methotrexate complexes of recombinant human dihydrofolate reductase.

Crystal structures of Escherichia coli and Lactobacillus casei dihydrofolate reductase refined at 1.7 A resolution. I. General features and binding of methotrexate.

1982 • 737 citations

Crystalline Dihydropteroylglutamic Acid

1960 • 461 citations

Polyglutamation of methotrexate. Is methotrexate a prodrug?

1985 • 460 citations

A short sequence in the p60src N terminus is required for p60src myristylation and membrane association and for cell transformation.

1984 • 447 citations

Mutation of NH2-terminal glycine of p60src prevents both myristoylation and morphological transformation.

1985 • 386 citations

Dihydrofolate Reductase: X-ray Structure of the Binary Complex with Methotrexate

1977 • 323 citations

Hepatic Aldehyde Oxidase

1962 • 287 citations

Dihydrofolate reductase from Lactobacillus casei. X-ray structure of the enzyme methotrexate.NADPH complex.

1978 • 229 citations

Methotrexate, a high-affinity pseudosubstrate of dihydrofolate reductase

1979 • 223 citations

Structure-activity relationships of dihydrofolated reductase inhibitors

1984 • 154 citations

The effect of methotrexate on intracellular folate pools in human MCF-7 breast cancer cells. Evidence for direct inhibition of purine synthesis.

1986 • 140 citations

Fine structural mapping of a critical NH2-terminal region of p60src.

1985 • 129 citations

Folate metabolism in mammalian cells in culture. I Partial characterization of the folate derivatives present in L1210 mouse leukemia cells.

1976 • 116 citations

Intracellular pharmacokinetics of methotrexate polyglutamates in human breast cancer cells. Selective retention and less dissociable binding of 4-NH2-10-CH3-pteroylglutamate4 and 4-NH2-10-CH3-pteroylglutamate5 to dihydrofolate reductase.

1983 • 105 citations

Kinetics of substrate, coenzyme, and inhibitor binding to Escherichia coli dihydrofolate reductase

1981 • 103 citations

Relationship of cellular folate cofactor pools to the activity of 5-fluorouracil.

1983 • 96 citations

Acquired methotrexate resistance in lymphoblasts resulting from altered kinetic properties of dihydrofoltate reductase

1977 • 95 citations

Intrinsic Resistance to Methotrexate of Cultured Mammalian Cells in Relation to the Inhibition Kinetics of Their Dihydrofolate Reductases

1976 • 91 citations

Mechanism of inhibition of dihydrofolate reductases from bacterial and vertebrate sources by various classes of folate analogues

1986 • 86 citations

Carbon-13 nuclear magnetic resonance study of protonation of methotrexate and aminopterin bound to dihydrofolate reductase

1981 • 86 citations

The effects on 4-aminoantifolates on 5-formyltetrahydrofolate metabolism in L1210 cells. A biochemical basis of the selectivity of leucovorin rescue.

1987 • 83 citations

Protonated state of methotrexate, trimethoprim, and pyrimethamine bound to dihydrofolate reductase

1983 • 81 citations

Methotrexate bioavailability after oral and intramuscular administration in children

1987 • 76 citations

6 Recent Progress in the Medicinal Chemistry of 2,4-Diaminopyrimidines

1982 • 73 citations

Kinetics of ligand binding to dihydrofolate reductase: binary complex formation with NADPH and coenzyme analogues

1978 • 73 citations

Role of aspartate 27 in the binding of methotrexate to dihydrofolate reductase from Escherichia coli.

1988 • 72 citations

A program for efficient integration of rate equations and least-squares fitting of chemical reaction data

1972 • 65 citations

Ultraviolet difference-spectroscopic studies of substrate and inhibitor binding to Lactobacillus casei dihydrofolate reductase

1978 • 60 citations

Synthesis and properties of 7-hydroxymethotrexate polyglutamyl derivatives in Ehrlich ascites tumor cells in vitro.

1984 • 55 citations

Purification and characterization of dihydrofolate reductase from methotrexate-resistant human lymphoblastoid cells

1983 • 53 citations

A soluble dihydrofolate reductase from human placenta: Purification and properties

1971 • 51 citations

Inhibition of dihydrofolate reductase from bacterial and vertebrate sources by folate, aminopterin, methotrexate and their 5-deaza analogues

1984 • 50 citations

Kinetics of ternary complex formation between dihydrofolate reductase, coenzyme, and inhibitors

1980 • 50 citations

The inhibition of dihydrofolate reductase by folate analogues: Structural requirements for slow- and tight-binding inhibition

1980 • 49 citations

Kinetics of 7-hydroxy-methotrexate after high-dose methotrexate therapy

1985 • 47 citations

Interaction of methotrexate, folates, and pyridine nucleotides with dihydrofolate reductase: calorimetric and spectroscopic binding studies.

1978 • 45 citations

Enzymatic synthesis of polyglutamate derivatives of 7-hydroxymethotrexate

1984 • 45 citations

Antifolate polyglutamylation and competitive drug displacement at dihydrofolate reductase as important elements in leucovorin rescue in L1210 cells.

1986 • 45 citations

The pH-dependence of the binding of dihydrofolate and substrate analogues to dihydrofolate reductase from Escherichia coli

1983 • 43 citations

Spectral Changes Associated with Binding of Folate Compounds to Bacteriophage T4 Dihydrofolate Reductase

1972 • 38 citations

Dihydrofolate reductase from a resistant subline of the L1210 lymphoma. Purification by affinity chromatography and ultraviolet difference spectrophotometric and circular dichroic studies

1977 • 37 citations

Lack of Dihydrofolate Reductase in Human Tumor and Leukemia CellsIn Vivo

1985 • 33 citations

Protonation of methotrexate bound to the catalytic site of dihydrofolate reductase from lactobacillus casei

1981 • 30 citations

Methotrexate and folate binding to dihydrofolate reductase. Separate characterization of the pteridine and p-aminobenzoyl binding sites by resonance Raman spectroscopy

1981 • 30 citations

Properties of dihydrofolate reductase from a methotrexate-resistant subline of human KB cells and comparison with enzyme from KB parent cells and mouse S180 AT/3000 cells.

1982 • 28 citations

A Comparison by QSAR, Crystallography, and Computer Graphics of the Inhibition of Various Dihydrofolate Reductases by 5‐(X‐Benzyl)‐2,4‐diamino‐pyrimidines

1982 • 27 citations

Role of isomerization of initial complexes in the binding of inhibitors to dihydrofolate reductase

1985 • 25 citations

Analysis of Methotrexate and 7-Hydroxymethotrexate by High-Performance Liquid Chromatography and Preliminary Clinical Studies

1982 • 23 citations

Cytotoxicity, uptake, polyglutamate formation, and antileukemic effects of 8-deaza analogues of methotrexate and aminopterin in mice.

1988 • 23 citations

Effects on dihydrofolate reductase of methotrexate metabolites and intracellular folates formed following methotrexate exposure of human breast cancer cells

1987 • 22 citations

Binding of methotrexate to Escherichia coli dihydrofolate reductase as measured by visible and ultraviolet resonance Raman spectroscopy

1978 • 21 citations

High-Dose Methotrexate for Osteosarcoma: Toxicity and Clinical Results

1983 • 20 citations

Genetic engineering vol. 3

1983 • 20 citations

Quantitative structure-selectivity relationships. Comparison of the inhibition of Escherichia coli and bovine liver dihydrofolate reductase by 5-(substituted-benzyl)-2,4-diaminopyrimidines

1981 • 12 citations

Binding of methotrexate to dihydrofolate reductase and its relation to protonation of the ligand

1985 • 11 citations

Federal Reorganization: Science and Technology

1977 • 2 citations