Catalytic Properties of 26 S and 20 S Proteasomes and Radiolabeling of MB1, LMP7, and C7 Subunits Associated with Trypsin-like and Chymotrypsin-like Activities

Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4

1970 • 253,849 citations

A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye Binding

1976 • 225,752 citations

A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding

1976 • 200,127 citations

Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.

1979 • 54,907 citations

Improved silver staining of plant proteins, RNA and DNA in polyacrylamide gels

1987 • 4,186 citations

Inhibitors of the proteasome block the degradation of most cell proteins and the generation of peptides presented on MHC class I molecules

1994 • 2,480 citations

STRUCTURE AND FUNCTIONS OF THE 20S AND 26S PROTEASOMES

1996 • 2,408 citations

Structure of 20S proteasome from yeast at 2.4Å resolution

1997 • 2,211 citations

Inhibition of Proteasome Activities and Subunit-Specific Amino-Terminal Threonine Modification by Lactacystin

1995 • 1,601 citations

Crystal Structure of the 20 S Proteasome from the Archaeon T. acidophilum at 3.4 Å Resolution

1995 • 1,513 citations

A new solid-state reagent to iodinate proteins

1982 • 893 citations

Ubiquitin, proteasomes, and the regulation of intracellular protein degradation

1995 • 862 citations

Proteolysis, proteasomes and antigen presentation

1992 • 576 citations

MHC Class I Expression in Mice Lacking the Proteasome Subunit LMP-7

1994 • 520 citations

The multicatalytic and 26 S proteases.

1993 • 476 citations

Identification, purification, and characterization of a protein activator (PA28) of the 20 S proteasome (macropain).

1992 • 439 citations

Proteinase yscE, the yeast proteasome/multicatalytic-multifunctional proteinase: mutants unravel its function in stress induced proteolysis and uncover its necessity for cell survival.

1991 • 421 citations

Proteasomes: multicatalytic proteinase complexes

1993 • 395 citations

Purification of an 11 S regulator of the multicatalytic protease.

1992 • 384 citations

Evidence for the presence of five distinct proteolytic components in the pituitary multicatalytic proteinase complex. Properties of two components cleaving bonds on the carboxyl side of branched chain and small neutral amino acids

1993 • 323 citations

Coordinated Dual Cleavages Induced by the Proteasome Regulator PA28 Lead to Dominant MHC Ligands

1996 • 287 citations

PRE2, highly homologous to the human major histocompatibility complex-linked RING10 gene, codes for a yeast proteasome subunit necessary for chrymotryptic activity and degradation of ubiquitinated proteins.

1993 • 260 citations

Multiple forms of the 20 S multicatalytic and the 26 S ubiquitin/ATP-dependent proteases from rabbit reticulocyte lysate.

1992 • 229 citations

Displacement of housekeeping proteasome subunits by MHC-encoded LMPs: a newly discovered mechanism for modulating the multicatalytic proteinase complex.

1994 • 220 citations

Subpopulations of proteasomes in rat liver nuclei, microsomes and cytosol

1996 • 172 citations

Proteasome Subunits X and Y Alter Peptidase Activities in Opposite Ways to the Interferon-γ-induced Subunits LMP2 and LMP7

1996 • 164 citations

Proteasome components with reciprocal expression to that of the MHC-encoded LMP proteins

1994 • 157 citations

Proteolysis: The proteasome: a protein-degrading organelle?

1995 • 155 citations

The PRE4 gene codes for a subunit of the yeast proteasome necessary for peptidylglutamyl-peptide-hydrolyzing activity. Mutations link the proteasome to stress- and ubiquitin-dependent proteolysis.

1993 • 149 citations

Subunit arrangement in the human 20S proteasome

1997 • 121 citations

PRE5 and PRE6, the Last Missing Genes Encoding 20S Proteasome Subunits from Yeast? Indication for a Set of 14 Different Subunits in the Eukaryotic Proteasome Core

1994 • 121 citations

Demonstration that a human 26S proteolytic complex consists of a proteasome and multiple associated protein components and hydrolyzes ATP and ubiquitin‐ligated proteins by closely linked mechanisms

1992 • 115 citations

Evaluation of isoelectric focusing running conditions during two-dimensional isoelectric focusing/sodium dodecyl sulfate-polyacrylamide gel electrophoresis: Variation of gel patterns with changing conditions and optimized isoelectric focusing conditions

1984 • 114 citations

Inhibition of the proteolytic activity of the multicatalytic proteinase complex (proteasome) by substrate-related peptidyl aldehydes.

1994 • 112 citations

Proteasomes and Antigen Processing

1997 • 106 citations

LMP2+ proteasomes are required for the presentation of specific antigens to cytotoxic T lymphocytes

1995 • 97 citations

Purification and Characterization of the 26S Proteasome Complex Catalyzing ATP-Dependent Breakdown of Ubiquitin-Ligated Prot from Rat Liver1

1993 • 85 citations

A tight cluster of five unrelated human genes on chromosome 16q22.1

1993 • 83 citations

PRE3, highly homologous to the human major histocompatibility complex‐linked LMP2 (RING12) gene, codes for a yeast proteasome subunit necessary for the peptidylglutamyl‐peptide hydrolyzing activity

1994 • 80 citations

[24] Multicatalytic endopeptidase complex: Proteasome

1994 • 79 citations

Proteasome sequences in eubacteria

1994 • 67 citations

N‐Terminal sequence similarities between components of the multicatalytic proteinase complex

1990 • 56 citations

The multicatalytic proteinase complex (proteasome): structure and conformational changes associated with changes in proteolytic activity

1993 • 52 citations

Peptidylglutamyl-peptide hydrolase activity of the multicatalytic proteinase complex: evidence for a new high-affinity site, analysis of cooperative kinetics, and the effect of manganese ions

1992 • 50 citations

Use of serine‐protease inhibitors as probes for the different proteolytic activities of the rat liver multicatalytic proteinase complex

1992 • 44 citations

Properties of subunits of the multicatalytic proteinase complex revealed by the use of subunit-specific antibodies

1991 • 36 citations

A Novel Chymotrypsin like Component of the Multicatalytic Proteinase Complex Optimally Active at Acidic pH

1995 • 33 citations

Identification and localization of a cysteinyl residue critical for the trypsin-like catalytic activity of the proteasome

1992 • 32 citations

Reaction of proteasomes with peptidylchloromethanes and peptidyldiazomethanes

1993 • 25 citations

Catalytic components of proteasomes and the regulation of proteinase activity

1995 • 19 citations

Cleavage of Pro-X and Glu-X Bonds Catalyzed by the Branched Chain Amino Acid Preferring Activity of the Bovine Pituitary Multicatalytic Proteinase Complex (20S Proteasome)

1996 • 18 citations

Iodination of peptidyl chloromethyl ketones for protease affinity labels

1988 • 16 citations