Regulated Hyperaccumulation of Presenilin-1 and the “γ-Secretase” Complex

High-efficiency transformation of mammalian cells by plasmid DNA.

1987 • 5,377 citations

A presenilin-1-dependent γ-secretase-like protease mediates release of Notch intracellular domain

1999 • 2,051 citations

Two transmembrane aspartates in presenilin-1 required for presenilin endoproteolysis and γ-secretase activity

1999 • 1,880 citations

Notch-1 signalling requires ligand-induced proteolytic release of intracellular domain

1998 • 1,600 citations

GSK-3α regulates production of Alzheimer's disease amyloid-β peptides

2003 • 1,145 citations

Endoproteolysis of Presenilin 1 and Accumulation of Processed Derivatives In Vivo

1996 • 1,013 citations

Nicastrin modulates presenilin-mediated notch/glp-1 signal transduction and βAPP processing

2000 • 906 citations

Reconstitution of γ-secretase activity

2003 • 868 citations

Photoactivated γ-secretase inhibitors directed to the active site covalently label presenilin 1

2000 • 861 citations

The role of presenilin cofactors in the γ-secretase complex

2003 • 856 citations

aph-1 and pen-2 Are Required for Notch Pathway Signaling, γ-Secretase Cleavage of βAPP, and Presenilin Protein Accumulation

2002 • 795 citations

γ-Secretase is a membrane protein complex comprised of presenilin, nicastrin, aph-1, and pen-2

2003 • 744 citations

MUTANT GENES IN FAMILIAL ALZHEIMER'S DISEASE AND TRANSGENIC MODELS

1998 • 576 citations

Presenilin 1 is linked with γ-secretase activity in the detergent solubilized state

2000 • 526 citations

Transition-state analogue inhibitors of γ-secretase bind directly to presenilin-1

2000 • 520 citations

γ-Secretase, notch, Aβ and alzheimer's disease: Where do the presenilins fit in?

2002 • 505 citations

Total inactivation of γ–secretase activity in presenilin-deficient embryonic stem cells

2000 • 480 citations

Presenilin‐dependent γ‐secretase processing of β‐amyloid precursor protein at a site corresponding to the S3 cleavage of Notch

2001 • 471 citations

Protease nexin-II, a potent anti-chymotrypsin, shows identity to amyloid β-protein precursor

1989 • 437 citations

Presenilins are required for γ-secretase cleavage of β-APP and transmembrane cleavage of Notch-1

2000 • 407 citations

APH-1 is a multipass membrane protein essential for the Notch signaling pathway in Caenorhabditis elegans embryos

2002 • 406 citations

The Presenilin 1 Protein Is a Component of a High Molecular Weight Intracellular Complex That Contains β-Catenin

1998 • 373 citations

The Proteolytic Fragments of the Alzheimer's Disease-associated Presenilin-1 Form Heterodimers and Occur as a 100–150-kDa Molecular Mass Complex

1998 • 329 citations

Generation of APLP2 KO Mice and Early Postnatal Lethality in APLP2/APP Double KO Mice

1997 • 327 citations

Evidence That Levels of Presenilins (PS1 and PS2) Are Coordinately Regulated by Competition for Limiting Cellular Factors

1997 • 321 citations

Metabolism of the “Swedish” Amyloid Precursor Protein Variant in Neuro2a (N2a) Cells

1996 • 308 citations

A Loss of Function Mutation of Presenilin-2 Interferes with Amyloid β-Peptide Production and Notch Signaling

1999 • 306 citations

Presenilin Proteins Undergo Heterogeneous Endoproteolysis between Thr291and Ala299and Occur as Stable N- and C-Terminal Fragments in Normal and Alzheimer Brain Tissue

1997 • 289 citations

Distinct Intramembrane Cleavage of the β-Amyloid Precursor Protein Family Resembling γ-Secretase-like Cleavage of Notch

2001 • 289 citations

Identification and transport of full-length amyloid precursor proteins in rat peripheral nervous system

1993 • 280 citations

PEN-2 Is an Integral Component of the γ-Secretase Complex Required for Coordinated Expression of Presenilin and Nicastrin

2002 • 276 citations

Cell Surface Presenilin-1 Participates in the γ-Secretase-like Proteolysis of Notch

1999 • 268 citations

The Transmembrane Aspartates in Presenilin 1 and 2 Are Obligatory for γ-Secretase Activity and Amyloid β-Protein Generation

2000 • 253 citations

Presenilin and nicastrin regulate each other and determine amyloid β-peptide production via complex formation

2002 • 232 citations

PEN-2 and APH-1 Coordinately Regulate Proteolytic Processing of Presenilin 1

2003 • 228 citations

Endoproteolytic Cleavage and Proteasomal Degradation of Presenilin 2 in Transfected Cells

1997 • 213 citations

Mammalian APH-1 Interacts with Presenilin and Nicastrin and Is Required for Intramembrane Proteolysis of Amyloid-β Precursor Protein and Notch

2002 • 212 citations

Characterization of a Presenilin-mediated Amyloid Precursor Protein Carboxyl-terminal Fragment γ

2001 • 207 citations

Endoproteolytic Processing and Stabilization of Wild-type and Mutant Presenilin

1997 • 205 citations

γ-Secretase activity requires the presenilin-dependent trafficking of nicastrin through the Golgi apparatus but not its complex glycosylation

2003 • 194 citations

Presenilin-1 affects trafficking and processing of βAPP and is targeted in a complex with nicastrin to the plasma membrane

2002 • 191 citations

Presenilin 1 Is Required for Maturation and Cell Surface Accumulation of Nicastrin

2002 • 184 citations

Stable Association of Presenilin Derivatives and Absence of Presenilin Interactions with APP

1998 • 177 citations

Nicastrin Is Required for Assembly of Presenilin/γ-Secretase Complexes to Mediate Notch Signaling and for Processing and Trafficking of β-Amyloid Precursor Protein in Mammals

2003 • 176 citations

Nicastrin Is Required for γ-Secretase Cleavage of the Drosophila Notch Receptor

2002 • 168 citations

Mature Glycosylation and Trafficking of Nicastrin Modulate Its Binding to Presenilins

2002 • 157 citations

Nicastrin is required for Presenilin-mediated transmembrane cleavage in Drosophila

2001 • 151 citations

Furin mediates enhanced production of fibrillogenic ABri peptides in familial British dementia

1999 • 150 citations

APH-1 Interacts with Mature and Immature Forms of Presenilins and Nicastrin and May Play a Role in Maturation of Presenilin·Nicastrin Complexes

2003 • 150 citations

Hyperaccumulation of FAD-linked presenilin 1 variants in vivo

1997 • 145 citations

Evidence for phosphorylation and oligomeric assembly of presenilin 1

1997 • 145 citations

Drosophila Nicastrin Is Essential for the Intramembranous Cleavage of Notch

2002 • 124 citations

Aspartate Mutations in Presenilin and γ‐Secretase Inhibitors Both Impair Notch1 Proteolysis and Nuclear Translocation with Relative Preservation of Notch1 Signaling

2000 • 116 citations

Specificity of RNA maturation pathways: RNAs transcribed by RNA polymerase III are not substrates for splicing or polyadenylation.

1987 • 115 citations

Complex N-linked Glycosylated Nicastrin Associates with Active γ-Secretase and Undergoes Tight Cellular Regulation

2002 • 112 citations

Amyloid production and deposition in mutant amyloid precursor protein and presenilin-1 yeast artificial chromosome transgenic mice

1999 • 104 citations

Multiple Effects of Aspartate Mutant Presenilin 1 on the Processing and Trafficking of Amyloid Precursor Protein

2001 • 103 citations

Notch and presenilins in vertebrates and invertebrates: implications for neuronal development and degeneration

2000 • 68 citations

Complex N‐glycosylated form of nicastrin is stabilized and selectively bound to presenilin fragments

2002 • 65 citations

Localization of presenilin–nicastrin complexes and γ‐secretase activity to the trans‐Golgi network

2003 • 59 citations

Aspartyl Protease Inhibitor Pepstatin Binds to the Presenilins of Alzheimer's Disease

2001 • 42 citations

Mutations at the P1′ position of Notch1 decrease intracellular domain stability rather than cleavage by γ-secretase

2002 • 16 citations