Structural and functional analyses of activating amino acid substitutions in the receiver domain of NtrC: Evidence for an activating surface

Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4

1970 • 253,849 citations

[6] Use of T7 RNA polymerase to direct expression of cloned genes

1990 • 6,681 citations

Towards a natural system of organisms: proposal for the domains Archaea, Bacteria, and Eucarya.

1990 • 5,831 citations

[19] Rapid and efficient site-specific mutagenesis without phenotypic selection

1987 • 5,413 citations

Calculation of protein extinction coefficients from amino acid sequence data

1989 • 5,198 citations

Escherichia Coli and Salmonella: Typhimurium Cellular and Molecular Biology

1987 • 4,385 citations

Spectroscopic Determination of Tryptophan and Tyrosine in Proteins^*

1967 • 3,423 citations

Arabidopsis Ethylene-Response Gene ETR1 : Similarity of Product to Two-Component Regulators

1993 • 1,478 citations

COMMUNICATION MODULES IN BACTERIAL SIGNALING PROTEINS

1992 • 1,449 citations

Rapid recording of 2D NMR spectra without phase cycling. Application to the study of hydrogen exchange in proteins

1989 • 1,395 citations

A two-component system that regulates an osmosensing MAP kinase cascade in yeast

1994 • 1,091 citations

Transcriptional regulation

1991 • 893 citations

Overcoming the overlap problem in the assignment of proton NMR spectra of larger proteins by use of three-dimensional heteronuclear proton-nitrogen-15 Hartmann-Hahn-multiple quantum coherence and nuclear Overhauser-multiple quantum coherence spectroscopy: application to interleukin 1.beta.

1989 • 840 citations

Two-Component Signal Transduction

1995 • 752 citations

Improved 3D triple-resonance NMR techniques applied to a 31 kDa protein

1992 • 732 citations

Covalent modification of the glnG product, NRI, by the glnL product, NRII, regulates the transcription of the glnALG operon in Escherichia coli.

1986 • 565 citations

Phosphorylation of three proteins in the signaling pathway of bacterial chemotaxis

1988 • 550 citations

Expression of sigma 54 (ntrA)-dependent genes is probably united by a common mechanism

1989 • 504 citations

Two-component signal transduction

1995 • 502 citations

Phosphorylation of bacterial response regulator proteins by low molecular weight phospho-donors.

1992 • 475 citations

Two-component regulatory systems responsive to environmental stimuli share strongly conserved domains with the nitrogen assimilation regulatory genes ntrB and ntrC.

1986 • 464 citations

Function of a Bacterial Activator Protein That Binds to Transcriptional Enhancers

1989 • 440 citations

A Yeast Protein Similar to Bacterial Two-Component Regulators

1993 • 431 citations

An efficient experiment for sequential backbone assignment of medium-sized isotopically enriched proteins

1992 • 391 citations

Protein kinase and phosphoprotein phosphatase activities of nitrogen regulatory proteins NTRB and NTRC of enteric bacteria: roles of the conserved amino-terminal domain of NTRC.

1988 • 365 citations

Products of nitrogen regulatory genes ntrA and ntrC of enteric bacteria activate glnA transcription in vitro: evidence that the ntrA product is a sigma factor.

1985 • 352 citations

Three-dimensional structure of CheY, the response regulator of bacterial chemotaxis

1989 • 350 citations

Acetyl phosphate and the activation of two-component response regulators.

1994 • 321 citations

The phosphorylated form of the enhancer-binding protein NTRC has an ATPase activity that is essential for activation of transcription

1991 • 315 citations

Structure of the Escherichia coli Response Regulator NarL^,

1996 • 313 citations

PROKARYOTIC SIGNAL TRANSDUCTION MEDIATED BY SENSOR AND REGULATOR PROTEIN PAIRS

1989 • 295 citations

Crystal structure of Escherichia coli CheY refined at 1.7-A resolution.

1991 • 293 citations

Structural conservation in the CheY superfamily

1993 • 288 citations

Sequence and domain relationships of ntrC and nifA from Klebsiella pneumoniae: homologies to other regulatory proteins.

1986 • 283 citations

Conserved aspartate residues and phosphorylation in signal transduction by the chemotaxis protein CheY.

1990 • 268 citations

Crystal structure of Escherichia coli CheY refined at 1.7-A resolution

1991 • 259 citations

Two-Component Signal Transduction Systems: Structure-Function Relationships and Mechanisms of Catalysis

2014 • 258 citations

Integration of multiple developmental signals in Bacillus subtilis through the Spo0A transcription factor.

1993 • 254 citations

Phosphorylation of nitrogen regulator I (NRI) of Escherichia coli.

1988 • 251 citations

Unusual Oligomerization Required for Activity of NtrC, a Bacterial Enhancer-Binding Protein

1997 • 239 citations

Practical aspects of 3D heteronuclear NMR of proteins

1989 • 222 citations

Role of phosphorylated metabolic intermediates in the regulation of glutamine synthetase synthesis in Escherichia coli

1992 • 217 citations

Structure of the magnesium-bound form of CheY and mechanism of phosphoryl transfer in bacterial chemotaxis

1993 • 215 citations

Long range hydrogen bond mediated effects in peptides: nitrogen-15 NMR study of gramicidin S in water and organic solvents

1984 • 199 citations

Suppression of cross-relaxation effects in TOCSY spectra via a modified DIPSI-2 mixing sequence

1992 • 192 citations

A Bacterial Enhancer Functions to Tether a Transcriptional Activator Near a Promoter

1990 • 190 citations

Molecular analysis of the flagellar switch protein FliM of Salmonella typhimurium

1992 • 185 citations

Maximizing gene expression from plasmid vectors containing the lambda PL promoter: strategies for overproducing transcription termination factor rho.

1985 • 184 citations

Oligomerization of NTRC at the glnA enhancer is required for transcriptional activation.

1993 • 182 citations

Glutamate at the Site of Phosphorylation of Nitrogen-regulatory Protein NTRC Mimics Aspartyl-Phosphate and Activates the Protein

1993 • 175 citations

The ?24/?12 promoter comes of age

1989 • 172 citations

Complete resonance assignment for the polypeptide backbone of interleukin 1.beta. using three-dimensional heteronuclear NMR spectroscopy

1990 • 168 citations

Modular structure of Fix J: homology of the transcriptional activator domain with the ‐35 binding domain of sigma factors

1991 • 149 citations

Phosphorylation of nitrogen regulator I of Escherichia coli induces strong cooperative binding to DNA essential for activation of transcription.

1992 • 145 citations

Response regulators of bacterial signal transduction systems: Selective domain shuffling during evolution

1995 • 142 citations

Magnesium Binding to the Bacterial Chemotaxis Protein CheY Results in Large Conformational Changes Involving its Functional Surface

1994 • 127 citations

Chemotaxis and phototaxis require a CheA histidine kinase in the archaeon Halobacterium salinarium.

1995 • 116 citations

Multiple forms of the CheB methylesterase in bacterial chemosensing.

1985 • 110 citations

The function of isolated domains and chimaeric proteins constructed from the transcriptional activators NifA and NtrC of Klebsiella pneumoniae

1990 • 110 citations

Three-dimensional solution structure of the N-terminal receiver domain of NTRC

1995 • 108 citations

Phosphorylation site of NtrC, a protein phosphatase whose covalent intermediate activates transcription

1992 • 98 citations

The central domain of Rhizobium leguminosarum DctD functions independently to activate transcription

1992 • 96 citations

Activation of the phosphosignaling protein CheY. II. Analysis of activated mutants by 19F NMR and protein engineering

1993 • 96 citations

A chemotactic signaling surface on CheY defined by suppressors of flagellar switch mutations

1992 • 93 citations

Phosphorylation and dephosphorylation of the NarQ, NarX, and NarL proteins of the nitrate-dependent two-component regulatory system of Escherichia coli

1994 • 92 citations

Oxygen-regulated in vitro transcription of Rhizobium meliloti nifA and fixK genes

1993 • 87 citations

Tyrosine 106 of CheY plays an important role in chemotaxis signal transduction in Escherichia coli

1996 • 84 citations

Constitutive mutations of Agrobacterium tumefaciens transcriptional activator virG

1992 • 82 citations

Signal transduction in chemotaxis. A propagating conformation change upon phosphorylation of CheY

1994 • 80 citations

Effects of Phosphorylation, Mg2+, and Conformation of the Chemotaxis Protein CheY on Its Binding to the Flagellar Switch Protein FliM

1994 • 76 citations

Uncoupled Phosphorylation and Activation in Bacterial Chemotaxis

1995 • 74 citations

Activating and inhibitory mutations in the regulatory domain of CheB, the methylesterase in bacterial chemotaxis.

1993 • 73 citations

Novel mutations that alter the regulation of sporulation in Bacillus subtilis

1990 • 70 citations

Activation of the phosphosignaling protein CheY. I. Analysis of the phosphorylated conformation by 19F NMR and protein engineering

1993 • 70 citations

Assignments, Secondary Structure, Global Fold, and Dynamics of Chemotaxis Y Protein Using Three- and Four-Dimensional Heteronuclear (13C,15N) NMR Spectroscopy

1994 • 69 citations

Structural alterations in the Bacillus subtilis Spo0A regulatory protein which suppress mutations at several spo0 loci

1990 • 68 citations

The Major Dimerization Determinants of the Nitrogen Regulatory Protein NTRC from Enteric Bacteria Lie in Its Carboxy-terminal Domain

1994 • 66 citations

Crystal structure of a phosphatase-resistant mutant of sporulation response regulator Spo0F from Bacillus subtilis

1996 • 66 citations

Constitutive Forms of the Enhancer-binding Protein NtrC: Evidence that Essential Oligomerization Determinants Lie in the Central Activation Domain

1995 • 65 citations

Purification of the alternative sigma factor, sigma 54, from Salmonella typhimurium and characterization of sigma 54-holoenzyme.

1991 • 64 citations

Alterations of highly conserved residues in the regulatory domain of nitrogen regulator I (NtrC) of Escherichia coli

1993 • 62 citations

Intramolecular signal transduction within the FixJ transcriptional activator:in vitroevidence for the inhibitory effect of the phosphorylatable regulatory domain

1994 • 62 citations

Rhizobium meliloti DctD, a σ54‐dependent transcriptional activator, may be negatively controlled by a subdomain in the C‐terminal end of its two‐component receiver module

1994 • 54 citations

Characterization of a virG mutation that confers constitutive virulence gene expression in Agrobacterium

1993 • 52 citations

Mutations that affect control of the methylesterase activity of CheB, a component of the chemotaxis adaptation system in Escherichia coli

1990 • 51 citations

Localized perturbations in CheY structure monitored by NMR identify a CheA binding interface

1995 • 36 citations

¹H,¹⁵N, and¹³C backbone chemical shift assignments, secondary structure, and magnesium-binding characteristics of thebacillus subtilisresponse regulator, SpoOF, determined by heteronuclear high-resolution NMR

1995 • 31 citations

New Suppressor Mutation sur0B of spo0B and spo0F Mutations in Bacillus subtilis

1988 • 27 citations

Heteronuclear-Correlation NMR Spectroscopy with Simultaneous Isotope Filtration, Quadrature Detection, and Sensitivity Enhancement Using z Rotations

1994 • 27 citations

A mutation in the receiver domain of the Agrobacterium tumefaciens transcriptional regulator VirG increases its affinity for operator DNA

1994 • 17 citations

Two transcriptionally active OmpR mutants that do not require phosphorylation by EnvZ in an Escherichia coli cell-free system

1992 • 5 citations