Uncoupled Phosphorylation and Activation in Bacterial Chemotaxis

A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye Binding

1976 • 225,752 citations

A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding

1976 • 200,127 citations

Solvent content of protein crystals

1968 • 7,954 citations

Escherichia coli and Salmonella typhimurium: Cellular and molecular biology

1988 • 3,957 citations

Chemotaxis in Escherichia coli analysed by Three-dimensional Tracking

1972 • 2,129 citations

COMMUNICATION MODULES IN BACTERIAL SIGNALING PROTEINS

1992 • 1,449 citations

Traitement statistique des erreurs dans la determination des structures cristallines

1952 • 1,284 citations

The Gradient-Sensing Mechanism in Bacterial Chemotaxis

1972 • 906 citations

Interactive computer graphics: FRODO

1985 • 848 citations

Signal transduction schemes of bacteria

1993 • 781 citations

Two-Component Signal Transduction

1995 • 752 citations

Stereochemically restrained refinement of macromolecular structures

1985 • 614 citations

Phosphorylation of three proteins in the signaling pathway of bacterial chemotaxis

1988 • 550 citations

The use of an imaging proportional counter in macromolecular crystallography

1987 • 494 citations

Phosphorylation of bacterial response regulator proteins by low molecular weight phospho-donors.

1992 • 475 citations

Change in direction of flagellar rotation is the basis of the chemotactic response in Escherichia coli

1974 • 462 citations

Structure, function and properties of antibody binding sites

1991 • 426 citations

Phosphorylation-dependent binding of a signal molecule to the flagellar switch of bacteria.

1993 • 425 citations

Three-dimensional structure of CheY, the response regulator of bacterial chemotaxis

1989 • 350 citations

MERLOT, an integrated package of computer programs for the determination of crystal structures by molecular replacement

1988 • 348 citations

Acetyl phosphate and the activation of two-component response regulators.

1994 • 321 citations

Identification of the site of phosphorylation of the chemotaxis response regulator protein, CheY

1989 • 303 citations

Crystal structure of Escherichia coli CheY refined at 1.7-A resolution.

1991 • 293 citations

Structural conservation in the CheY superfamily

1993 • 288 citations

Conserved aspartate residues and phosphorylation in signal transduction by the chemotaxis protein CheY.

1990 • 268 citations

Crystal structure of Escherichia coli CheY refined at 1.7-A resolution

1991 • 259 citations

Assembly and function of a quaternary signal transduction complex monitored by surface plasmon resonance

1993 • 252 citations

Regulation of an Enzyme by Phosphorylation at the Active Site

1990 • 246 citations

Incorporation of fast Fourier transforms to speed restrained least-squares refinement of protein structures

1987 • 221 citations

Structure of the magnesium-bound form of CheY and mechanism of phosphoryl transfer in bacterial chemotaxis

1993 • 215 citations

Roles of the highly conserved aspartate and lysine residues in the response regulator of bacterial chemotaxis

1991 • 215 citations

Reconstitution of signaling in bacterial chemotaxis

1987 • 212 citations

Protein phosphorylation is involved in bacterial chemotaxis.

1987 • 198 citations

Molecular analysis of the flagellar switch protein FliM of Salmonella typhimurium

1992 • 185 citations

Histidine and aspartate phosphorylation: two-component systems and the limits of homology

1994 • 181 citations

Sensory transduction in bacterial chemotaxis involves phosphotransfer between CHE proteins

1988 • 181 citations

Divalent metal ion binding to the CheY protein and its significance to phosphotransfer in bacterial chemotaxis

1990 • 168 citations

Structural heterogeneity in protein crystals

1986 • 163 citations

Correlation between phosphorylation of the chemotaxis protein CheY and its activity at the flagellar motor

1992 • 154 citations

Ribonuclease T1 with free recognition and catalytic site: Crystal structure analysis at 1.5 Å resolution

1991 • 144 citations

Molecular components of bacterial chemotaxis

1987 • 143 citations

Response Regulators CheB and CheY Exhibit Competitive Binding to the Kinase CheA

1995 • 133 citations

Design and structural analysis of alternative hydrophobic core packing arrangements in bacteriophage T4 lysozyme

1992 • 130 citations

Overexpression and sequence of the Escherichia coli cheY gene and biochemical activities of the CheY protein

1984 • 129 citations

Magnesium Binding to the Bacterial Chemotaxis Protein CheY Results in Large Conformational Changes Involving its Functional Surface

1994 • 127 citations

Expression of CheA fragments which define domains encoding kinase, phosphotransfer, and CheY binding activities

1993 • 117 citations

Crystal Structural Analysis of Mutations in the Hydrophobic Cores of Barnase

1993 • 111 citations

Crystal structure of myoglobin form a synthetic gene

1990 • 110 citations

Restoration of flagellar clockwise rotation in bacterial envelopes by insertion of the chemotaxis protein CheY.

1986 • 109 citations

Identification of a phosphorylation site and functional analysis of conserved aspartic acid residues of OmpR, a transcriptional activator for ompF and ompC in Escherichia coli

1993 • 103 citations

Activation of the phosphosignaling protein CheY. II. Analysis of activated mutants by 19F NMR and protein engineering

1993 • 96 citations

A chemotactic signaling surface on CheY defined by suppressors of flagellar switch mutations

1992 • 93 citations

The role of a signaling protein in bacterial sensing: behavioral effects of increased gene expression.

1984 • 89 citations

Regulation of isocitrate dehydrogenase by phosphorylation involves no long-range conformational change in the free enzyme.

1990 • 85 citations

Tyrosine 106 of CheY plays an important role in chemotaxis signal transduction in Escherichia coli

1996 • 84 citations

Signal transduction in chemotaxis. A propagating conformation change upon phosphorylation of CheY

1994 • 80 citations

Phosphorylation-dependent binding of the chemotaxis signal molecule CheY to its phosphatase, CheZ

1994 • 77 citations

Acetyladenylate or its derivative acetylates the chemotaxis protein CheY in vitro and increases its activity at the flagellar switch

1992 • 77 citations

Effects of Phosphorylation, Mg2+, and Conformation of the Chemotaxis Protein CheY on Its Binding to the Flagellar Switch Protein FliM

1994 • 76 citations

Mutations that Affect Separate Functions of OmpR the Phosphorylated Regulator of Porin Transcription in Escherichia coli

1993 • 74 citations

A Physicist Looks at Bacterial Chemotaxis

1988 • 73 citations

[15] Phosphorylation assays for proteins of the two-component regulatory system controlling chemotaxis in Escherichia coli

1991 • 71 citations

Bacterial chemotaxis signaling complexes: formation of a CheA/CheW complex enhances autophosphorylation and affinity for CheY.

1991 • 71 citations

Novel mutations that alter the regulation of sporulation in Bacillus subtilis

1990 • 70 citations

Activation of the phosphosignaling protein CheY. I. Analysis of the phosphorylated conformation by 19F NMR and protein engineering

1993 • 70 citations

Assignments, Secondary Structure, Global Fold, and Dynamics of Chemotaxis Y Protein Using Three- and Four-Dimensional Heteronuclear (13C,15N) NMR Spectroscopy

1994 • 69 citations

Oligomerization of the Phosphatase CheZ Upon Interaction with the Phosphorylated Form of CheY

1996 • 53 citations

Protein Phosphorylation and Bacterial Chemotaxis

1988 • 52 citations

Change in direction of flagellar rotation in Escherichia coli mediated by acetate kinase

1993 • 42 citations

Mutants with Defective Phosphatase Activity Show No Phosphorylation-dependent Oligomerization of CheZ

1996 • 42 citations

Mutations Leading to Altered CheA Binding Cluster on a Face of CheY

1995 • 38 citations

¹H‐ and ¹⁵N‐NMR assignment and solution structure of the chemotactic Escherichia coli Che Y protein

1993 • 37 citations

Signal Transduction and Osmoregulation in Escherichia coli: A Novel Mutant of the Positive Regulator, OmpR, That Functions in a Phosphorylation-Independent Manner1

1992 • 31 citations

The Three-dimensional Structure of Two Mutants of the Signal Transduction Protein CheY Suggest its Molecular Activation Mechanism

1996 • 30 citations

Intramolecular second-site revertants to the phosphorylation site mutation in OmpR, a kinase-dependent transcriptional activator in Escherichia coli

1991 • 26 citations

Three-dimensional structure of chemotactic che Y protein in aqueous solution by nuclear magnetic resonance methods

1995 • 24 citations

Isolation of phosphorylation‐deficient mutants of the Rhizobium Meliloti two‐component regulatory protein, FixJ

1992 • 20 citations

Bivalent-metal binding to CheY protein. Effect on protein conformation

1992 • 20 citations

A pulsed diffusion technique for the growth of protein crystals for X-ray diffraction

1975 • 19 citations

Crystallization and preliminary characterization of CheY, a chemotaxis control protein from Escherichia coli.

1986 • 12 citations

Specificity and affinity of binding of phosphate-containing compounds to CheY protein

1992 • 6 citations