Crystal structure of the large fragment of Thermus aquaticus DNA polymerase I at 2.5-A resolution: structural basis for thermostability.

Primer-Directed Enzymatic Amplification of DNA with a Thermostable DNA Polymerase

1988 • 17,059 citations

Improved methods for building protein models in electron density maps and the location of errors in these models

1991 • 12,831 citations

Enzymatic Amplification of β-Globin Genomic Sequences and Restriction Site Analysis for Diagnosis of Sickle Cell Anemia

1985 • 9,128 citations

[21] Specific synthesis of DNA in vitro via a polymerase-catalyzed chain reaction

1987 • 5,745 citations

Protein folding and association: Insights from the interfacial and thermodynamic properties of hydrocarbons

1991 • 5,267 citations

AREAS, VOLUMES, PACKING, AND PROTEIN STRUCTURE

1977 • 2,631 citations

Crystal Structure at 3.5 Å Resolution of HIV-1 Reverse Transcriptase Complexed with an Inhibitor

1992 • 1,887 citations

Crystal structure of human immunodeficiency virus type 1 reverse transcriptase complexed with double-stranded DNA at 3.0 A resolution shows bent DNA.

1993 • 1,097 citations

Structural basis for the 3′-5′ exonuclease activity of Escherichia coli DNA polymerase I: a two metal ion mechanism.

1991 • 1,059 citations

PCR amplification of up to 35-kb DNA with high fidelity and high yield from lambda bacteriophage templates.

1994 • 1,043 citations

Structure of large fragment of Escherichia coli DNA polymerase I complexed with dTMP

1985 • 855 citations

Calculation of the total electrostatic energy of a macromolecular system: Solvation energies, binding energies, and conformational analysis

1988 • 829 citations

DNA sequencing with Thermus aquaticus DNA polymerase and direct sequencing of polymerase chain reaction-amplified DNA.

1988 • 775 citations

Using known substructures in protein model building and crystallography.

1986 • 766 citations

Amino‐aromatic interactions in proteins

1986 • 752 citations

Enhanced protein thermostability from site-directed mutations that decrease the entropy of unfolding.

1987 • 738 citations

Electrostatic Effects in Proteins

1978 • 734 citations

Structures of ternary complexes of rat DNA polymerase beta, a DNA template-primer, and ddCTP.

1994 • 694 citations

Enzymatic Synthesis of Deoxyribonucleic Acid

1972 • 594 citations

High-fidelity amplification using a thermostable DNA polymerase isolated from Pyrococcus furiosus

1991 • 569 citations

Thermal stability and protein structure

1979 • 518 citations

Structure of DNA Polymerase I Klenow Fragment Bound to Duplex DNA

1993 • 473 citations

Crystal Structure of Rat DNA Polymerase β: Evidence for a Common Polymerase Mechanism

1994 • 464 citations

Stereochemical basis of heat stability in bacterial ferredoxins and in haemoglobin A2

1975 • 420 citations

High fidelity DNA synthesis by theThermus aquaticusDNA polymerase

1990 • 393 citations

Alternative packing arrangements in the hydrophobic core of λrepresser

1989 • 375 citations

Hydrophobic stabilization in T4 lysozyme determined directly by multiple substitutions of Ile 3

1988 • 375 citations

Crystal structure of bacteriophage T7 RNA polymerase at 3.3 Å resolution

1993 • 358 citations

Current approaches to macromolecular crystallization

1990 • 351 citations

MUTATIONAL EFFECTS ON PROTEIN STABILITY

1989 • 311 citations

High-level expression, purification, and enzymatic characterization of full-length Thermus aquaticus DNA polymerase and a truncated form deficient in 5' to 3' exonuclease activity.

1993 • 286 citations

Engineering protein thermal stability

1989 • 276 citations

Computer Simulations of the Diffusion of a Substrate to an Active Site of an Enzyme

1987 • 224 citations

The fidelity of Taq polymerase catalyzing PCR is improved by an N-terminal deletion

1992 • 207 citations

The crystal structure of a mutant protein with altered but improved hydrophobic core packing.

1994 • 110 citations

Role of proline residues in human lysozyme stability: a scanning calorimetric study combined with x-ray structure analysis of proline mutants

1992 • 71 citations