The ubiquitin-like protein Smt3p is activated for conjugation to other proteins by an Aos1p/Uba2p heterodimer

A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae.

1989 • 8,705 citations

Methods in yeast genetics

1979 • 7,824 citations

Methods in Yeast Genetics

1988 • 4,905 citations

New yeast-Escherichia coli shuttle vectors constructed with in vitro mutagenized yeast genes lacking six-base pair restriction sites

1988 • 2,903 citations

A positive selection for mutants lacking orotidine-5′-phosphate decarboxylase activity in yeast: 5-fluoro-orotic acid resistance

1984 • 2,401 citations

The HPV-16 E6 and E6-AP complex functions as a ubiquitin-protein ligase in the ubiquitination of p53

1993 • 2,329 citations

In Vivo Half-Life of a Protein Is a Function of Its Amino-Terminal Residue

1986 • 1,942 citations

UBIQUITIN-DEPENDENT PROTEIN DEGRADATION

1996 • 1,680 citations

A Multiubiquitin Chain Is Confined to Specific Lysine in a Targeted Short-Lived Protein

1989 • 1,463 citations

[19] Targeting, disruption, replacement, and allele rescue: Integrative DNA transformation in yeast

1991 • 1,369 citations

How Proteolysis Drives the Cell Cycle

1996 • 1,277 citations

A Small Ubiquitin-Related Polypeptide Involved in Targeting RanGAP1 to Nuclear Pore Complex Protein RanBP2

1997 • 1,221 citations

A novel ubiquitin-like modification modulates the partitioning of the Ran-GTPase-activating protein RanGAP1 between the cytosol and the nuclear pore complex.

1996 • 1,161 citations

Components of ubiquitin-protein ligase system. Resolution, affinity purification, and role in protein breakdown.

1983 • 1,105 citations

Protein ubiquitination involving an E1–E2–E3 enzyme ubiquitin thioester cascade

1995 • 960 citations

The yeast polyubiquitin gene is essential for resistance to high temperatures, starvation, and other stresses

1987 • 850 citations

A GAL10-CYC1 hybrid yeast promoter identifies the GAL4 regulatory region as an upstream site.

1982 • 575 citations

Arabidopsis auxin-resistance gene AXR1 encodes a protein related to ubiquitin-activating enzyme E1

1993 • 531 citations

The yeast ubiquitin genes: a family of natural gene fusions.

1987 • 500 citations

Role of a ubiquitin-conjugating enzyme in degradation of S- and M-phase cyclins

1995 • 492 citations

Two nuclear mutations that block mitochondrial protein import in yeast.

1984 • 490 citations

The interferon-inducible 15-kDa ubiquitin homolog conjugates to intracellular proteins.

1992 • 420 citations

Evidence that the MIF2 gene of Saccharomyces cerevisiae encodes a centromere protein with homology to the mammalian centromere protein CENP-C.

1995 • 410 citations

The recognition component of the N-end rule pathway.

1990 • 388 citations

PIC 1, a novel ubiquitin-like protein which interacts with the PML component of a multiprotein complex that is disrupted in acute promyelocytic leukaemia.

1996 • 347 citations

The mechanism of ubiquitin activating enzyme. A kinetic and equilibrium analysis.

1982 • 324 citations

Protection against Fas/APO-1- and tumor necrosis factor-mediated cell death by a novel protein, sentrin.

1996 • 323 citations

"Covalent affinity" purification of ubiquitin-activating enzyme.

1982 • 310 citations

Purification of his-tagged proteins in non-denaturing conditions suggests a convenient method for protein interaction studies

1991 • 289 citations

UBA 1: an essential yeast gene encoding ubiquitin-activating enzyme.

1991 • 281 citations

Identification of Subunits of the Anaphase-Promoting Complex of Saccharomyces cerevisiae

1996 • 263 citations

Immunoaffinity purification of FLAG epitope-tagged bacterial alkaline phosphatase using a novel monoclonal antibody and peptide elution.

1994 • 246 citations

The Saccharomyces cerevisiae DNA repair gene RAD23 encodes a nuclear protein containing a ubiquitin-like domain required for biological function.

1993 • 246 citations

Surface hydrophobic residues of multiubiquitin chains essential for proteolytic targeting.

1996 • 240 citations

Stress resistance in Saccharomyces cerevisiae is strongly correlated with assembly of a novel type of multiubiquitin chain.

1994 • 238 citations

Novel Multiubiquitin Chain Linkages Catalyzed by the Conjugating Enzymes E2EPF and RAD6 Are Recognized by 26 S Proteasome Subunit 5

1996 • 221 citations

An Essential Yeast Gene Encoding a Homolog of Ubiquitin-activating Enzyme

1995 • 207 citations

Identification of BIME as a Subunit of the Anaphase-Promoting Complex

1996 • 200 citations

RanBP2 associates with Ubc9p and a modified form of RanGAP1

1997 • 190 citations

Fusion of GAL4-VP16 to a steroid-binding domain provides a tool for gratuitous induction of galactose-responsive genes in yeast

1993 • 188 citations

Preferential Modification of Nuclear Proteins by a Novel Ubiquitin-like Molecule

1997 • 173 citations

Rna1p, a Ran/TC4 GTPase activating protein, is required for nuclear import.

1995 • 168 citations

Yeast ubiquitin-like genes are involved in duplication of the microtubule organizing center.

1996 • 165 citations

Efficient random mutagenesis method with adjustable mutation frequency by use of PCR and dITP

1993 • 164 citations

Cloning and functional analysis of the ubiquitin-specific protease gene UBP1 of Saccharomyces cerevisiae

1991 • 161 citations

Genetic analysis of the ubiquitin system

1991 • 158 citations

Associations of UBE2I with RAD52, UBL1, p53, and RAD51 Proteins in a Yeast Two-Hybrid System

1996 • 135 citations

Plasmid Vectors Carrying the Replication Origin of Filamentous Single-Stranded Phages

1987 • 127 citations

APP-BP1, a Novel Protein That Binds to the Carboxyl-terminal Region of the Amyloid Precursor Protein

1996 • 118 citations

Peptide-Mass Profiles of Polyvinylidene Difluoride-Bound Proteins by Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry in the Presence of Nonionic Detergents

1996 • 113 citations

Structural genes for thiamine biosynthetic enzymes (thiCEFGH) in Escherichia coli K-12

1993 • 110 citations

Ubiquitin adenylate: structure and role in ubiquitin activation

1983 • 95 citations

Expression of a ubiquitin derivative that conjugates to protein irreversibly produces phenotypes consistent with a ubiquitin deficiency.

1992 • 82 citations

Multiple forms of ubiquitin-activating enzyme E1 from wheat. Identification of an essential cysteine by in vitro mutagenesis.

1992 • 78 citations

The Saccharomyces cerevisiae DNA Repair Gene RAD23 Encodes a Nuclear Protein Containing a Ubiquitin-Like Domain Required for Biological Function

1993 • 66 citations

Two proteins encoded at the chlA locus constitute the converting factor of Escherichia coli chlA1

1989 • 59 citations

Characterisation of Schizosaccharomyces pombe rad31, a UBA-related gene required for DNA damage tolerance

1997 • 54 citations

Functional Characterization of the Ubiquitin Variant Encoded by the Baculovirus Autographa californica

1996 • 45 citations

Uncoupling ubiquitin-protein conjugation from ubiquitin-dependent proteolysis by use of .beta.,.gamma.-nonhydrolyzable ATP analogs

1991 • 43 citations

Immunochemical Probes of Ubiquitin Pool Dynamics

1988 • 38 citations

Ubiquitin Activation and Ligation

1988 • 13 citations

Deleted Work

1955 • 0 citations