An Essential Yeast Gene Encoding a Homolog of Ubiquitin-activating Enzyme

A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae.

1989 • 8,705 citations

Methods in yeast genetics

1979 • 7,824 citations

Rapid and efficient site-specific mutagenesis without phenotypic selection.

1985 • 7,573 citations

Green Fluorescent Protein as a Marker for Gene Expression

1994 • 6,572 citations

Methods in Yeast Genetics

1988 • 4,905 citations

The codon adaptation index-a measure of directional synonymous codon usage bias, and its potential applications

1987 • 3,628 citations

New yeast-Escherichia coli shuttle vectors constructed with in vitro mutagenized yeast genes lacking six-base pair restriction sites

1988 • 2,903 citations

THE FUNCTION OF HEAT-SHOCK PROTEINS IN STRESS TOLERANCE: DEGRADATION AND REACTIVATION OF DAMAGED PROTEINS

1993 • 2,177 citations

The ubiquitin-proteasome proteolytic pathway

1994 • 1,778 citations

High-frequency transformation of yeast: autonomous replication of hybrid DNA molecules.

1979 • 1,504 citations

The two-hybrid system: a method to identify and clone genes for proteins that interact with a protein of interest.

1991 • 1,489 citations

THE UBIQUITIN SYSTEM FOR PROTEIN DEGRADATION

1992 • 1,402 citations

[19] Targeting, disruption, replacement, and allele rescue: Integrative DNA transformation in yeast

1991 • 1,369 citations

Yeast RNA Polymerase II Genes: Isolation with Antibody Probes

1983 • 1,084 citations

Purification of a RAS-responsive adenylyl cyclase complex from Saccharomyces cerevisiae by use of an epitope addition method.

1988 • 983 citations

Protein ubiquitination involving an E1–E2–E3 enzyme ubiquitin thioester cascade

1995 • 960 citations

The yeast polyubiquitin gene is essential for resistance to high temperatures, starvation, and other stresses

1987 • 850 citations

THE UBIQUITIN-CONJUGATION SYSTEM

1992 • 561 citations

Ubiquitin dependence of selective protein degradation demonstrated in the mammalian cell cycle mutant ts85

1984 • 533 citations

Arabidopsis auxin-resistance gene AXR1 encodes a protein related to ubiquitin-activating enzyme E1

1993 • 531 citations

Thermolability of ubiquitin-activating enzyme from the mammalian cell cycle mutant ts85

1984 • 522 citations

The N-end rule

1992 • 464 citations

The mechanism and functions of ATP‐dependent proteases in bacterial and animal cells

1992 • 450 citations

Ubiquitination

1991 • 430 citations

Ubiquitin-activating enzyme. Mechanism and role in protein-ubiquitin conjugation.

1982 • 413 citations

Predicted nucleotide-binding properties of p21 protein and its cancer-associated variant

1983 • 390 citations

An efficient transformation procedure enabling long‐term storage of competent cells of various yeast genera

1991 • 379 citations

Inhibition of proteolysis and cell cycle progression in a multiubiquitination-deficient yeast mutant.

1994 • 347 citations

"Covalent affinity" purification of ubiquitin-activating enzyme.

1982 • 310 citations

Purification of his-tagged proteins in non-denaturing conditions suggests a convenient method for protein interaction studies

1991 • 289 citations

UBA 1: an essential yeast gene encoding ubiquitin-activating enzyme.

1991 • 281 citations

Protein Degradation in Plants

1993 • 273 citations

The N-end rule is mediated by the UBC2(RAD6) ubiquitin-conjugating enzyme.

1991 • 268 citations

[8] Putting the HO gene to work: Practical uses for mating-type switching

1991 • 254 citations

Immunoaffinity purification of FLAG epitope-tagged bacterial alkaline phosphatase using a novel monoclonal antibody and peptide elution.

1994 • 246 citations

Natural substrates of the Ubiquitin proteolytic pathway

1991 • 238 citations

Epitope-tagged ubiquitin. A new probe for analyzing ubiquitin function.

1991 • 212 citations

A Chinese hamster cell cycle mutant arrested at G2 phase has a temperature-sensitive ubiquitin-activating enzyme, E1.

1988 • 173 citations

Galactose as a gratuitous inducer of GAL gene expression in yeasts growing on glucose

1989 • 168 citations

RNA14 and RNA15 Proteins as Components of a Yeast Pre-mRNA 3′-End Processing Factor

1994 • 161 citations

Homology of a candidate spermatogenic gene from the mouse Y chromosome to the ubiquitin-activating enzyme El

1991 • 156 citations

Separation of factors required for cleavage and polyadenylation of yeast pre-mRNA.

1992 • 136 citations

Molecular cloning, sequence, and tissue distribution of the human ubiquitin-activating enzyme E1.

1991 • 129 citations

A candidate spermatogenesis gene on the mouse Y chromosome is homologous to ubiquitin-activating enzyme E1

1991 • 127 citations

Cloning and expression of the essential gene for poly(A) polymerase from S. cerevisiae

1991 • 127 citations

Yeast RAD6 encoded ubiquitin conjugating enzyme mediates protein degradation dependent on the N-end-recognizing E3 enzyme.

1991 • 115 citations

Chemical synthesis and expression of a cassette adapted ubiquitin gene.

1987 • 105 citations

Involvement of chlA, E, M, and N loci in Escherichia coli molybdopterin biosynthesis

1987 • 100 citations

Ubiquitin adenylate: structure and role in ubiquitin activation

1983 • 95 citations

A gene in the chromosomal region 3p21 with greatly reduced expression in lung cancer is similar to the gene for ubiquitin-activating enzyme.

1993 • 93 citations

Substrate properties of site-specific mutant ubiquitin protein (G76A) reveal unexpected mechanistic features of ubiquitin-activating enzyme (E1).

1994 • 88 citations

N-recognin/Ubc2 interactions in the N-end rule pathway

1993 • 88 citations

Ubiquitin and intracellular protein degradation

1992 • 84 citations

Multiple forms of ubiquitin-activating enzyme E1 from wheat. Identification of an essential cysteine by in vitro mutagenesis.

1992 • 78 citations

Cloning of ubiquitin activating enzyme from wheat and expression of a functional protein in Escherichia coli.

1990 • 69 citations

Utility of polyhistidine-tagged ubiquitin in the purification of ubiquitin-protein conjugates and as an affinity ligand for the purification of ubiquitin-specific hydrolases.

1993 • 58 citations

Null alleles of SAC7 suppress temperature-sensitive actin mutations in Saccharomyces cerevisiae.

1990 • 47 citations

Analysis of the yeastSPT3gene and identification of its product, a positive regulator of Ty trarscription

1986 • 32 citations

Complementation by a Cloned Human Ubiquitin-Activating Enzyme E1 of the S-Phase-Arrested Mouse FM3A Cell Mutant with Thermolabile E1.

1992 • 25 citations

Cloning and sequence of a functionally active cDNA encoding the mouse ubiquitin-activating enzyme E1

1992 • 24 citations

tsBN75 and tsBN423, temperature-sensitive X-linked mutants of the BHK21 cell line, can be complemented by the ubiquitin-activating enzyme E1 cDNA

1992 • 11 citations

The yeastSUFSframeshift suppressor encodes a mutant glycine tRNA_ccc

1988 • 11 citations

The yeastSUF3frameshift suppressor encodes a mutant glycine tRNA_ccc

1988 • 5 citations