DNA sequence and characterization of the S. cerevisiae gene encoding adenylate cyclase

DNA sequencing with chain-terminating inhibitors

1977 • 69,181 citations

Detection of specific sequences among DNA fragments separated by gel electrophoresis

1975 • 33,059 citations

A simple method for displaying the hydropathic character of a protein

1982 • 22,471 citations

Transformation of intact yeast cells treated with alkali cations

1983 • 7,688 citations

Purification of Biologically Active Globin Messenger RNA by Chromatography on Oligothymidylic acid-Cellulose

1972 • 6,922 citations

Efficientin vitrosynthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter

1984 • 6,459 citations

The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers

1982 • 6,153 citations

RNA molecular weight determinations by gel electrophoresis under denaturing conditions, a critical reexamination

1977 • 3,548 citations

[12] One-step gene disruption in yeast

1983 • 3,302 citations

3′ Non-coding region sequences in eukaryotic messenger RNA

1976 • 3,001 citations

Buffer gradient gels and 35S label as an aid to rapid DNA sequence determination.

1983 • 2,456 citations

G proteins and dual control of adenylate cyclase

1984 • 1,811 citations

The molecular biology of the yeast saccharomyces

1982 • 1,165 citations

A putative signal peptidase recognition site and sequence in eukaryotic and prokaryotic signal peptides

1983 • 1,143 citations

In yeast, RAS proteins are controlling elements of adenylate cyclase

1985 • 1,142 citations

[32] Oligonucleotide-directed mutagenesis of DNA fragments cloned into M13 vectors

1983 • 1,093 citations

Transformation in yeast: Development of a hybrid cloning vector and isolation of the can1 gene

1979 • 1,080 citations

DNA sequence required for efficient transcription termination in yeast

1982 • 1,014 citations

The sequence 5′-AAUAAA-3′ forms part of the recognition site for polyadenylation of late SV40 mRNAs

1981 • 819 citations

The primary structure of the Saccharomyces cerevisiae gene for alcohol dehydrogenase.

1982 • 558 citations

Genes in S. cerevisiae encoding proteins with domains homologous to the mammalian ras proteins

1984 • 471 citations

Genetic analysis of yeast RAS1 and RAS2 genes

1984 • 460 citations

Construction of a colicin E1-R factor composite plasmid in vitro: means for amplification of deoxyribonucleic acid

1975 • 449 citations

Evidence for an intron-contained sequence required for the splicing of yeast RNA polymerase II transcripts

1983 • 396 citations

Differential activation of yeast adenylate cyclase by wild type and mutant RAS proteins

1985 • 365 citations

Isolation and characterization of yeast mutants deficient in adenylate cyclase and cAMP-dependent protein kinase.

1982 • 351 citations

The organization and transcription of the galactose gene cluster of Saccharomyces

1981 • 350 citations

ras-Related gene sequences identified and isolated from Saccharomyces cerevisiae

1983 • 345 citations

Pattern recognition in nucleic acid sequences. I. A general method for finding local homologies and symmetries

1982 • 331 citations

Functional homology of mammalian and yeast RAS genes

1985 • 324 citations

Periodicity of leucine and tandem repetition of a 24-amino acid segment in the primary structure of leucine-rich alpha 2-glycoprotein of human serum.

1985 • 313 citations

A sequence downstream of AAUAAA is required for rabbit β-globin mRNA 3′-end formation

1984 • 251 citations

Experimental Manipulation of Gene Expression

1983 • 213 citations

Mammalian and Yeast ras Gene Products: Biological Function in Their Heterologous Systems

1985 • 209 citations

Sequences responsible for transcription termination on a gene segment in Saccharomyces cerevisiae.

1984 • 154 citations

Isolation and sequence of the gene for iso-2-cytochrome c in Saccharomyces cerevisiae.

1980 • 153 citations

Genetic Characterization of Mutations Which Affect Catabolite-Sensitive Operons in Escherichia coli , Including Deletions of the Gene for Adenyl Cyclase

1973 • 141 citations

A sequence downstream of A-A-U-A-A-A is required for formation of simian virus 40 late mRNA 3' termini in frog oocytes.

1985 • 139 citations

A physical, genetic and transcriptional map of the cloned his3 gene region of Saccharomyces cerevisiae

1980 • 116 citations

Vectors for High-Level, Inducible Expression of Cloned Genes in Yeast

1983 • 114 citations

Isolation of the gene encoding adenylate cyclase in Saccharomyces cerevisiae.

1985 • 108 citations

Identification of the structural gene and nonsense alleles for adenylate cyclase in Saccharomyces cerevisiae

1984 • 101 citations

A product of yeast RAS2 gene is a guanine nucleotide binding protein.

1984 • 86 citations

Deletion mapping a eukaryotic promoter.

1981 • 79 citations

The complete nucleotide sequence of the adenylate cyclase gene ofEscherichia coli

1984 • 71 citations

Yeast mating pheromone alpha factor inhibits adenylate cyclase.

1980 • 62 citations

Characterization of cyclic AMP-requiring yeast mutants altered in the regulatory subunit of protein kinase.

1982 • 60 citations

Characterization of cyclic AMP-requiring yeast mutants altered in the catalytic subunit of protein kinase.

1984 • 60 citations

Plasmid vectors designed for high-efficiency expression controlled by the portable recA promoteroperator of Escherichia coli

1984 • 37 citations

Expression and characterization of ras mRNAs from Saccharomyces cerevisiae.

1984 • 30 citations