An improved positive selection plasmid vector constructed by ollgonucleotide mediated mutagenesis

A rapid alkaline extraction procedure for screening recombinant plasmid DNA

1979 • 15,511 citations

The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers

1982 • 6,153 citations

Construction and characterization of new cloning vehicles. II. A multipurpose cloning system. 1977.

1992 • 4,683 citations

A complementation analysis of the restriction and modification of DNA in Escherichia coli

1969 • 3,866 citations

Nucleotide sequence of bacteriophage λ DNA

1982 • 1,171 citations

Construction and characterization of new cloning vehicles IV. Deletion derivatives of pBR322 and pBR325

1980 • 616 citations

Local mutagenesis: a method for generating viral mutants with base substitutions in preselected regions of the viral genome.

1978 • 282 citations

[21] Transformation and preservation of competent bacterial cells by freezing

1979 • 278 citations

Synthesis of oligodeoxyribonucleotides on silica gel support

1981 • 130 citations

DNA sequence of the bacteriophage λ cI gene

1978 • 121 citations

Efficient correction of a mutation by use of chemically synthesized DNA.

1978 • 112 citations

A plasmid cloning vector for the direct selection of strains carrying recombinant plasmids

1981 • 109 citations

A plasmid cloning vehicle allowing a positive selection for inserted fragments

1980 • 105 citations

Expression of the EcoRI restriction-modification system and the construction of positive-selection cloning vectors

1982 • 73 citations

Site-specific mutagenesis using oligodeoxyribonucleotides: isolation of a phenotypically silent φX174 mutant, with a specific nucleotide deletion, at very high efficiency

1980 • 57 citations

Positive-selection cloning vehicle useful for overproduction of hybrid proteins

1983 • 54 citations

Restriction map of the antibiotic resistance plasmid R1 drd-19 and its derivatives pKN102 (R1drd-19B2) and R1drd-16 for the enzymes BamHI, HindIII, EcoRI and SalI

1978 • 53 citations

[62] Sequencing analysis of short DNA fragments

1980 • 41 citations

A rapid and efficient method for region- and strand-specific mutagenesis of cloned DNA.

1982 • 37 citations

Synthesis of mixed oligodeoxyribonucleotides following the solid phase method

1982 • 33 citations

Direct selection of recombinant plasmids in Bacillus subtilis

1982 • 29 citations

Plasmid vectors for positive selection of DNA inserts controlled by the λ pl promoter, repressor and antitermination function

1981 • 26 citations

Construction of an HpaI and HindII plasmid vector allowing direct selection of transformants harboring recombinant plasmids

1979 • 21 citations