Expression of the EcoRI restriction-modification system and the construction of positive-selection cloning vectors

Methods in Enzymology , Vol

1966 • 26,261 citations

A rapid alkaline extraction procedure for screening recombinant plasmid DNA

1979 • 15,511 citations

Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I

1977 • 12,015 citations

Construction and characterization of new cloning vehicles. II. A multipurpose cloning system. 1977.

1992 • 4,683 citations

Construction and characterization of amplifiable multicopy DNA cloning vehicles derived from the P15A cryptic miniplasmid

1978 • 4,472 citations

A complementation analysis of the restriction and modification of DNA in Escherichia coli

1969 • 3,866 citations

Construction and characterization of new cloning vehicle. II. A multipurpose cloning system

1977 • 3,829 citations

Colony hybridization: a method for the isolation of cloned DNAs that contain a specific gene.

1975 • 3,483 citations

REGULATORY SEQUENCES INVOLVED IN THE PROMOTION AND TERMINATION OF RNA TRANSCRIPTION

1979 • 2,730 citations

Analysis of restriction fragments of T7 DNA and determination of molecular weights by electrophoresis in neutral and alkaline gels

1977 • 1,607 citations

Rat Insulin Genes: Construction of Plasmids Containing the Coding Sequences

1977 • 1,252 citations

Construction and characterization of new cloning vehicles III. Derivatives of plasmid pBR322 carrying unique Eco RI sites for selection of Eco RI generated recombinant DNA molecules

1978 • 752 citations

DNA Modification and Restriction

1969 • 624 citations

A simple method for the preparation of large quantities of pure plasmid DNA

1975 • 586 citations

Gene amplification and analysis

1986 • 517 citations

Nucleotide sequence analysis of the chloramphenicol resistance transposon Tn9

1979 • 494 citations

Restriction Endonuclease

1976 • 405 citations

Organization of transcriptional signals in plasmids pBR322 and pACYC184.

1981 • 365 citations

Restriction and modification enzymes and their recognition sequences

1981 • 317 citations

Enzymatic end-to-end joining of DNA molecules

1973 • 311 citations

Construction and characterization of the chloramphenicol-resistance gene cartridge: A new approach to the transcriptional mapping of extrachromosomal elements

1982 • 307 citations

[10] Elution of DNA from agarose gels after electrophoresis

1979 • 266 citations

Extracellular nucleases of Pseudomonas BAL 31. III. Use of the double-strand deoxyriboexonuclease activity as the basis of a convenient method for the mapping of fragments of DNA produced by cleavage with restriction enzymes

1978 • 234 citations

The plasmid cloning vector pBR325 contains a 482 base-pair-long inverted duplication

1981 • 213 citations

In vitro mutagenesis of a circular DNA molecule by using synthetic restriction sites.

1978 • 200 citations

Sequence analysis of the DNA encoding the Eco RI endonuclease and methylase.

1981 • 194 citations

DNA sequences of structural genes for Eco RI DNA restriction and modification enzymes.

1981 • 167 citations

Mutagenesis of plasmid DNA with hydroxylamine: Isolation of mutants of multi-copy plasmids

1976 • 151 citations

A restriction endonuclease analysis of the bacterial plasmid controlling the ecoRI restriction and modification of DNA.

1976 • 137 citations

The hsd (host specificity) genes of E. coli K12

1980 • 130 citations

Transformation ofEscherichia coliC600 by plasmid DNA at different phases of growth

1979 • 124 citations

Cloning the modification methylase gene of Bacillus sphaericus R in Escherichia coli

1980 • 116 citations

Cloning of restriction and modification genes in E. coli: The HhaII system from Haemophilus haemolyticus

1978 • 109 citations

A plasmid cloning vehicle allowing a positive selection for inserted fragments

1980 • 105 citations

Molecular cloning of fragments of bacteriophage T4 DNA

1977 • 93 citations

Cloning and expression in streptomyces lividans of antibiotic resistance genes derived from Escherichia coli

1981 • 81 citations

Cloning and expression of the Pst I restriction-modification system in Escherichia coli.

1981 • 66 citations

Restriction and modification enzymes and their recognition sequences

1982 • 51 citations

The transcription termination site at the end of the early region of bacteriophage T7 DNA

1980 • 30 citations

Characterisation of plasmids coding for the restriction endonuclease EcoRI

1976 • 27 citations

Plasmid vectors for positive selection of DNA inserts controlled by the λ pl promoter, repressor and antitermination function

1981 • 26 citations

Construction of an HpaI and HindII plasmid vector allowing direct selection of transformants harboring recombinant plasmids

1979 • 21 citations

The structure and function of the Eco RI restriction endonuclease.

1981 • 15 citations

Structure and properties of the region of homology between plasmids pMB1 and ColE1

1981 • 12 citations

DNA Modification and Restriction

1974 • 12 citations