Methotrexate-resistant Chinese hamster ovary cells contain a dihydrofolate reductase with an altered affinity for methotrexate

PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENT

1951 • 319,299 citations

High resolution two-dimensional electrophoresis of proteins.

1975 • 19,515 citations

A Film Detection Method for Tritium‐Labelled Proteins and Nucleic Acids in Polyacrylamide Gels

1974 • 8,693 citations

Quantitative Film Detection of 3H and 14C in Polyacrylamide Gels by Fluorography

1975 • 4,693 citations

Measurement of protein-binding phenomena by gel filtration

1962 • 1,275 citations

Two Types of Ribosome in Mouse–Hamster Hybrid Cells

1971 • 577 citations

On the nature of hereditable variation in cultured somatic cells

1976 • 497 citations

Specific Binding of 4-Amino Folic Acid Analogues by Folic Acid Reductase

1961 • 389 citations

Isolation and partial characterization of three methotrexate-resistant phenotypes from Chinese hamster ovary cells

1976 • 237 citations

Effect of substrate decomposition on the spectrophotometric assay of dihydrofolate reductase

1967 • 235 citations

Methotrexate, a high-affinity pseudosubstrate of dihydrofolate reductase

1979 • 223 citations

Mutations causing charge alterations in regulatory subunits of the cAMP-dependent protein kinase of cultured S49 lymphoma cells

1977 • 222 citations

Peptide-Protein Interaction as Studied by Gel Filtration^*

1966 • 180 citations

Studies on the Inhibition of Dihydrofolate Reductase by the Folate Antagonists

1964 • 176 citations

Competitive protein binding assay for methotrexate.

1975 • 100 citations

Dihydrofolate reductase. Purification and characterization of the enzyme from an amethopterin-resistant mutant of Escherichia coli

1972 • 100 citations

Dihydrofolate reductase from amethopterin-resistant Lactobacillus casei

1972 • 93 citations

Analysis of HeLa cell hypoxanthine phosphoribosyltransferase mutants and revertants by two-dimensional polyacrylamide gel electrophoresis: evidence for silent gene activation.

1976 • 80 citations

Tissue-Specific Synthesis of Methotrexate Polyglutamates in the Rat

1975 • 80 citations

Dihydrofolate Reductase from the L1210R Murine Lymphoma. Fluorometric Measurements of the Interaction of the Enzyme with Coenzymes, Substrates, and Inhibitors^*

1966 • 76 citations

Evidence for functional hemizygosity at the Emtr locus in CHO cells through segregation analysis

1978 • 74 citations

Alpha-Amanitin resistance of RNA polymerase II in mutant Chinese hamster ovary cell lines.

1976 • 67 citations

Characterization of chicken liver dihydrofolate reductase after purification by affinity chromatography and isoelectric focusing

1977 • 55 citations

Methotrexate Binding to Dihydrofolate Reductase from a Methotrexate-resistant Strain of Escherichia coli

1973 • 53 citations

Emetine resistance in Chinese hamster cells is linked genetically with an altered 40S ribosomal subunit protein, S20.

1979 • 53 citations

Isolation and Characterization of the Multiple Forms of Dihydrofolate Reductase from Methotrexate-resistant Hamster Cells

1974 • 49 citations

Evidence obtained by induced mutation frequency analysis for functional hemizygosity at theemt locus in CHO cells

1979 • 42 citations

Genetic characterization of methotrexate-resistant chinese hamster ovary cells

1976 • 41 citations

Interconversion of the multiple forms of dihydrofolate reductase from amethopterin-resistant Lactobacillus casei

1971 • 40 citations

Alteration in structure of multifunctional protein from Chinese hamster ovary cells defective in pyrimidine biosynthesis

1979 • 38 citations

Purification and properties of dihydrofolate reductase from methotrexate-sensitive and methotrexate-resistant Chinese hamster ovary cells

1977 • 37 citations

Isolation of the soluble substrate recognition component of the dicarboxylate transport system of Escherichia coli.

1975 • 36 citations

Amethopterin-induced changes in the multiple forms of dihydrofolate reductase from L1210 cells

1970 • 34 citations

Tryptic peptide analysis of normal and mutant forms of hypoxanthine phosphoribosyltransferase from HeLa cells.

1977 • 29 citations

Evidence for variation in the number of functional gene copies at the Ama^R locus in chinese hamster cell lines

1978 • 28 citations

TPNH-dependent binding of amethopterin by dihydrofolate reductase from Lactobacillus casei

1972 • 26 citations

KINETIC STUDIES OF THE REACTION MECHANISM OF DIHYDROFOLATE REDUCTASE

1971 • 18 citations

DISCUSSION PAPER: ISOELECTRIC FOCUSING STUDIES ON DIHYDROFOLIC REDUCTASE

1971 • 7 citations

Reduced nicotinamide adenine dinucleotide phosphate-dependent binding of competitive inhibitors to dihydrofolate reductase.

1970 • 6 citations