Ligand interactions with eukaryotic translation initiation factor 2: role of the gamma-subunit.

[19] Rapid and efficient site-specific mutagenesis without phenotypic selection

1987 • 5,413 citations

The GTPase superfamily: conserved structure and molecular mechanism

1991 • 3,365 citations

TRANSLATIONAL CONTROL IN MAMMALIAN CELLS

1991 • 1,041 citations

Crystal Structure of the Ternary Complex of Phe-tRNA ^Phe , EF-Tu, and a GTP Analog

1995 • 846 citations

Phosphorylation of initiation factor 2α by protein kinase GCN2 mediates gene-specific translational control of GCN4 in yeast

1992 • 768 citations

GTP-binding domain: three consensus sequence elements with distinct spacing.

1987 • 602 citations

Mechanism and regulation of eukaryotic protein synthesis

1992 • 591 citations

[20] In vitro mutagenesis and plasmid shuffling: From cloned gene to mutant yeast

1991 • 584 citations

Crystal structure of active elongation factor Tu reveals major domain rearrangements

1993 • 533 citations

The crystal structure of elongation factor EF-Tu from Thermus aquaticus in the GTP conformation

1993 • 399 citations

The catalytic mechanism of guanine nucleotide exchange factor action and competitive inhibition by phosphorylated eukaryotic initiation factor 2.

1988 • 325 citations

Protein Phosphorylation Controls Translation Rates

1989 • 303 citations

Regulation of eukaryotic protein synthesis by initiation factors.

1993 • 300 citations

Refined structure of elongation factor EF-Tu from Escherichia coli

1992 • 287 citations

Mutations at a Zn(II) finger motif in the yeast elF-2β gene alter ribosomal start-site selection during the scanning process

1988 • 283 citations

[14] Cloning genes by complementation in yeast

1991 • 266 citations

Purification of the eukaryotic initiation factor 2-eukaryotic initiation factor 2B complex and characterization of its guanine nucleotide exchange activity during protein synthesis initiation.

1983 • 203 citations

Yeast translation initiation suppressor sui2 encodes the alpha subunit of eukaryotic initiation factor 2 and shares sequence identity with the human alpha subunit.

1989 • 201 citations

Translational control of GCN4: an in vivo barometer of initiation-factor activity

1994 • 183 citations

Positive regulatory interactions of the HIS4 gene of Saccharomyces cerevisiae.

1984 • 166 citations

Studies on Polynucleotides

1969 • 161 citations

Gene‐specific translational control of the yeast GCN4 gene by phosphorylation of eukaryotic initiation factor 2

1993 • 146 citations

Nucleotide regulation of a eukaryotic protein synthesis initiation complex

1975 • 143 citations

Mechanism of polypeptide chain initiation in eukaryotes and its control by phosphorylation of the alpha subunit of initiation factor 2.

1982 • 134 citations

GCN1, a translational activator of GCN4 in Saccharomyces cerevisiae, is required for phosphorylation of eukaryotic translation initiation factor 2 by protein kinase GCN2.

1993 • 128 citations

Modulation of tRNA(_i^Met), eIF-2, and eIF-2B Expression Shows that GCN4 Translation Is Inversely Coupled to the Level of eIF-2·GTP·Met-tRNA(_i^Met) Ternary Complexes

1995 • 124 citations

Structure of the β subunit of translational initiation factor elF-2

1988 • 122 citations

Cloning and sequencing of complementary DNAs encoding the alpha-subunit of translational initiation factor eIF-2. Characterization of the protein and its messenger RNA.

1987 • 110 citations

rasH mutants deficient in GTP binding.

1986 • 107 citations

Studies on the Polypeptide Elongation Factors from E. coli

1974 • 103 citations

The guanine nucleotide-exchange factor, eIF-2B

1994 • 101 citations

Mutations of the ras gene product p21 that abolish guanine nucleotide binding.

1986 • 91 citations

Characterization of GTP-dependent Met-tRNAf binding protein.

1977 • 89 citations

A segment of GCN4 mRNA containing the upstream AUG codons confers translational control upon a heterologous yeast transcript.

1987 • 89 citations

Evidence that GCD6 and GCD7, Translational Regulators of GCN4, Are Subunits of the Guanine Nucleotide Exchange Factor for eIF-2 in Saccharomyces cerevisiae

1993 • 88 citations

GCD11, a Negative Regulator of GCN4 Expression, Encodes the γ Subunit of eIF-2 in Saccharomyces cerevisiae

1993 • 84 citations

Molecular analysis of GCN3, a translational activator of GCN4: evidence for posttranslational control of GCN3 regulatory function.

1988 • 84 citations

A protein complex of translational regulators of GCN4 mRNA is the guanine nucleotide-exchange factor for translation initiation factor 2 in yeast.

1993 • 81 citations

Translation initiation factor eIF-2. Cloning and expression of the human cDNA encoding the gamma-subunit.

1994 • 70 citations

Mutations in GCD11, the structural gene for eIF-2 gamma in yeast, alter translational regulation of GCN4 and the selection of the start site for protein synthesis.

1995 • 68 citations

Cdc25p, the guanine nucleotide exchange factor for the Ras proteins of Saccharomyces cerevisiae, promotes exchange by stabilizing Ras in a nucleotide-free state

1994 • 65 citations

Positive Regulatory Interactions of the HIS4 Gene of Saccharomyces cerevisiae

1984 • 64 citations

Regulation of protein synthesis in eukaryotes. Mode of action of eRF, an eIF-2-recycling factor from rabbit reticulocytes involved in GDP/GTP exchange

1984 • 64 citations

The effect of Mg2+ and guanine nucleotide exchange factor on the binding of guanine nucleotides to eukaryotic initiation factor 2.

1988 • 55 citations

Kinetics and thermodynamics of the interaction of elongation factor Tu with elongation factor Ts, guanine nucleotides, and aminoacyl-tRNA.

1985 • 53 citations

MicroReview Control of translation initiation in Saccharomyces cerevisiae

1992 • 50 citations

GCN1, a Translational Activator of GCN4 in Saccharomyces cerevisiae, is Required for Phosphorylation of Eukaryotic Translation Initiation Factor 2 by Protein Kinase GCN2

1993 • 43 citations

Mutagenesis of bacterial elongation factor Tu at lysine 136

1989 • 42 citations

Inhibition of SDC25 C-domain-induced guanine-nucleotide exchange by guanine ring binding domain mutants of v-H-ras.

1993 • 40 citations

Histidine residues in elongation factor EF‐Tu from Escherichia coli protected by aminoacyl‐tRNA against photo‐oxidation

1984 • 37 citations

A functional analysis of the repeated methionine initiator tRNA genes (IMT) in yeast

1989 • 31 citations

The role of the β-subunit of initiation factor eIF-2 in initiation complex formation

1993 • 30 citations

Molecular Analysis of GCN3, a Translational Activator of GCN4: Evidence for Posttranslational Control of GCN3 Regulatory Function

1988 • 27 citations

The identification of a domain in Escherichia coli elongation factor Tu that interacts with elongation factor Ts.

1992 • 26 citations

Purification and properties of eukaryotic initiation factor 2 and its ancillary protein factor (Co-eIF-2A) from yeast Saccharomyces cerevisiae.

1985 • 24 citations

Modification of amino groups in EF-Tu . GTP and the ternary complex EF-Tu . GTP . valyl-tRNAVal

1984 • 24 citations

Close correlation between initiator methionyl-tRNA level and rate of protein synthesis during human lymphocyte growth cycle.

1981 • 22 citations

Amino acid sequence analysis of the β- and γ-subunits of eukaryotic initiation factor eIF-2. Identification of regions interacting with GTP

1991 • 22 citations

Isolation of eukaryotic initiation factor 2 from yeast Saccharomyces cerevisiae.

1981 • 21 citations

ras^H Mutants Deficient in GTP Binding

1986 • 18 citations

The conversion of eIF-2·GDP to eIF-2·GTP by eIF-2B requires Met-tRNAfMet

1991 • 16 citations

Kinetic constants in the functioning of eIF-2 and eIF-2B.

1987 • 5 citations

Kinetic parameters governing the formation of eIF-2.methionyl-tRNAi complexes in protein synthesis.

1990 • 2 citations

Control of initiation of eukaryotic protein synthesis by guanine nucleotides and methionyl-tRNAi.

1991 • 1 citations