A monocistronic transcript for a trypanosome variant surface glycoprotein.

Molecular cloning: A laboratory manual

1990 • 85,659 citations

Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose.

1980 • 8,711 citations

Firefly luciferase gene: structure and expression in mammalian cells.

1987 • 3,088 citations

Dideoxy sequencing method using denatured plasmid templates

1986 • 1,537 citations

New Culture Medium for Maintenance of Tsetse Tissues and Growth of Trypanosomatids*

1977 • 429 citations

Coupling of poly(A) site selection and trans-splicing in Leishmania.

1993 • 345 citations

Oligodeoxyribonucleotide ligation to single-stranded cDNAs: a new tool for cloning 5′ ends of mRNAs and for constructing cDNA libraries byin vitroamplification

1991 • 312 citations

Inactivation of transcription by UV irradiation of T. brucei provides evidence for a multicistronic transcription unit including a VSG gene

1987 • 307 citations

Cellular and Genetic Aspects of Antigenic Variation in Trypanosomes

1990 • 276 citations

The genes and transcripts of an antigen gene expression site from T. brucei

1989 • 257 citations

An analysis of cosmid clones of nuclear DNA fromTrypanosoma bruceishows that the genes for variant surface glycoproteins are clustered in the genome

1982 • 246 citations

Transcripts coding for variant surface glycoproteins of Trypanosoma brucei have a short, identical exon at their 5′ end

1982 • 243 citations

α-Amanitin-insensitive transcription of variant surface glycoprotein genes provides further evidence for discontinuous transcription in trypanosomes

1984 • 228 citations

Structure of the growing telomeres of trypanosomes

1984 • 227 citations

The anatomy and transcription of a telomeric expression site for variant-specific surface antigens in T. brucei

1987 • 218 citations

β-d-glucosyl-hydroxymethyluracil: A novel modified base present in the DNA of the parasitic protozoan T. brucei

1993 • 200 citations

Identification of a telomeric DNA sequence in Trypanosoma brucei

1984 • 190 citations

Polycistronic Transcripts in Trypanosomes and Their Accumulation During Heat Shock: Evidence for a Precursor Role in mRNA Synthesis

1988 • 174 citations

Coordinate transcription of variant surface glycoprotein genes and an expression site associated gene family in Trypanosoma brucei

1985 • 171 citations

Gene conversion as a mechanism for antigenic variation in Trypanosomes

1983 • 167 citations

The 35-Nucleotide Spliced Leader Sequence Is Common to All Trypanosome Messenger RNA's

1986 • 164 citations

Trypanosome mRNAs share a common 5′ spliced leader sequence

1984 • 163 citations

The transposition unit of variant surface glycoprotein gene 118 of Trypanosoma brucei

1983 • 138 citations

Transcription of the procyclic acidic repetitive protein genes of Trypanosoma brucei.

1990 • 137 citations

High frequency of antigenic variation inTrypanosoma brucei rhodesienseinfections

1989 • 136 citations

Anatomy of the parp gene promoter of Trypanosoma brucei.

1991 • 117 citations

Trypanosoma brucei contains two RNA polymerase II largest subunit genes with an altered C-terminal domain

1989 • 116 citations

Molecular biology of trypanosome antigenic variation

1985 • 114 citations

Alpha-amanitin-resistant transcription units in trypanosomes: a comparison of promoter sequences for a VSG gene expression site and for the ribosomal RNA genes

1991 • 112 citations

Transient activity assays of the Trypanosoma brucei variant surface glycoprotein gene promoter: control of gene expression at the posttranscriptional level.

1991 • 109 citations

Polycistronic transcripts in trypanosomes and their accumulation during heat shock: evidence for a precursor role in mRNA synthesis.

1988 • 107 citations

Apparent generation of a segmented mRNA from two separate tandem gene families inTrypanosoma cruzi

1985 • 106 citations

A novel flagellar Ca2+-binding Protein in Trypanosomes

1989 • 105 citations

The 5′-limit of transposition and upstream barren region of a trypanosome VSG gene: tandem 76 base-pair repeats flanking (TAA)₉₀

1984 • 104 citations

A possible role for the 3′-untranslated region in developmental regulation in Trypanosoma brucei

1993 • 99 citations

RNA polymerase I can mediate expression of CAT and neo protein-coding genes in Trypanosoma brucei.

1991 • 99 citations

Modification of telomeric DNA inTrypanosoma brucei;a role in antigenic variation?

1984 • 97 citations

Efficient production of functional mRNA mediated by RNA polymerase I in Trypanosoma brucei

1991 • 91 citations

Telomeric reciprocal recombination as a possible mechanism for antigenic variation in trypanosomes

1985 • 88 citations

Transcription of telomere repeats in protozoa.

1989 • 85 citations

Active late-appearing variable surface antigen genes in Trypanosoma equiperdum are constructed entirely from pseudogenes.

1989 • 82 citations

VSG gene 118 is transcribed from a cotransposed pol I-like promoter

1987 • 82 citations

Possible DNA modification in GC dinucleotides ofTrypanosoma bruceitelomeric sequences; relationship with antigen gene transcriptiond

1984 • 78 citations

The Promoter for the Procyclic Acidic Repetitive Protein (PARP) Genes of Trypanosoma brucei Shares Features with RNA Polymerase I Promoters

1992 • 78 citations

Surface epitope variation via mosaic gene formation is potential key to long-term survival of Trypanosoma brucei

1992 • 75 citations

Characterization of the RNA polymerases of Trypanosoma brucei: trypanosomal mRNAs are composed of transcripts derived from both RNA polymerase II and III.

1989 • 71 citations

A novel DNA nucleotide inTrypanosoma bruceionly present in the mammalian phase of the life-cycle

1991 • 68 citations

Antigenic diversity by the recombination of pseudogenes.

1989 • 66 citations

Molecular biology of trypanosome antigenic variation.

1985 • 65 citations

Characterization of the genes specifying two metacyclic variable antigen types in Trypanosoma brucei rhodesiense.

1984 • 62 citations

Stable expression of two variable surface glycoproteins by cloned Trypanosoma equiperdum

1986 • 62 citations

Characterization of VSG gene expression site promoters and promoter-associated DNA rearrangement events.

1991 • 62 citations

Point mutations are associated with a gene duplication leading to the bloodstream reexpression of a trypanosome metacyclic VSG

1993 • 55 citations

Linkage of the calmodulin and ubiquitin loci in Trypanosoma cruzi

1990 • 51 citations

The 5′ flanking sequence of a Trypanosoma brucei variable surface glycoprotein gene

1987 • 45 citations

Metacyclic Variant Surface Glycoprotein Genes of Trypanosoma brucei Subsp. rhodesiense Are Activated In Situ, and Their Expression Is Transcriptionally Regulated

1986 • 43 citations

The promoter for the procyclic acidic repetitive protein (PARP) genes of Trypanosoma brucei shares features with RNA polymerase I promoters.

1992 • 43 citations

Differential response to RNAtrans-splicing signals within the phosphoglycerate kinase gene cluster inTrypanosoma brucei

1993 • 41 citations

Trypanosoma brucei: enrichment by UV of intergenic transcripts from the variable surface glycoprotein gene expression site.

1989 • 40 citations

Identification of proteins encoded by variant surface glycoprotein expression site-associated genes in Trypanosoma brucei

1986 • 40 citations

Distinct, developmental stage-specific activation mechanisms of trypanosome VSG genes

1990 • 40 citations

The actin gene promoter ofTrypanosoma brucei

1991 • 37 citations

Characterization of trypanosome protein phosphatase 1 and 2A catalytic subunits

1991 • 35 citations

Isolation and Characterization of a new Serodeme of Trypanosoma Rhodesiense *

1979 • 33 citations

Duplicative activation mechanisms of two trypanosome telomeric VSG genes with structurally simple 5′ flanks

1990 • 33 citations

Genomic and transcriptional linkage of the genes for calmodulin, EF-hand 5 protein, and ubiquitin extension protein 52 in Trypanosoma brucei.

1993 • 32 citations

Molecular Biology of African Trypanosomes: Development of New Strategies to Combat an Old Disease

1992 • 31 citations

Independent expression of the metacyclic and bloodstream variable antigen repertoires ofTrypanosoma brucei rhodesiense

1986 • 30 citations

Control of antigenic variation in African trypanosomes.

1991 • 30 citations

Metacyclic variant surface glycoprotein genes of Trypanosoma brucei subsp. rhodesiense are activated in situ, and their expression is transcriptionally regulated.

1986 • 29 citations

Expression site associated genes of Trypanosoma brucei rhodesiense

1989 • 28 citations

TheTrypanosoma bruceiprotein phosphatase gene: polycistronic transcription with the RNA polymerase II largest subunit gene

1990 • 28 citations

High homology between variant surface glycoprotein gene expression sites of Trypanosoma brucei and Trypanosoma gambiense

1991 • 28 citations

The putative promoter for a metacyclic VSG gene in African trypanosomes

1995 • 28 citations

Characterization of VSG Gene Expression Site Promoters and Promoter-Associated DNA Rearrangement Events

1991 • 26 citations

Genetics of antigenic variation in African trypanosomes

1991 • 24 citations

Metacyclic form-specific variable surface glycoprotein-encoding genes of Trypanosoma (Nannomonas)congolense

1992 • 18 citations

Characterization of genes coding for two major metacyclic surface antigens in Trypanosoma brucei

1987 • 15 citations

Bloodstream and metacyclic variant surface glycoprotein gene expression sites of Trypanosoma brucei gambiense

1990 • 15 citations

Cellular And Genetic Aspects Of Antigenic Variation In Trypanosomes

1990 • 13 citations

Characterization and nomenclature of trypanosome serodemes and zymodemes: Report of a meeting held in Edinburgh, September 1978

1982 • 11 citations