Polycistronic Transcripts in Trypanosomes and Their Accumulation During Heat Shock: Evidence for a Precursor Role in mRNA Synthesis

Molecular Cloning: A Laboratory Manual

2001 • 133,517 citations

Molecular Cloning. A Laboratory Manual

1983 • 27,328 citations

Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I

1977 • 12,015 citations

Efficientin vitrosynthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter

1984 • 6,459 citations

A catalogue of splice junction sequences

1982 • 3,861 citations

Colony hybridization: a method for the isolation of cloned DNAs that contain a specific gene.

1975 • 3,483 citations

Determination of nucleic acid sequence homologies and relative concentrations by a dot hybridization procedure

1979 • 1,403 citations

Isolation of salivarian trypanosomes from man and other mammals using DEAE-cellulose

1970 • 1,349 citations

Transcriptional regulation of the ovalbumin and conalbumin genes by steroid hormones in chick oviduct.

1979 • 975 citations

Identification, purification and properties of clone-specific glycoprotein antigens constituting the surface coat ofTrypanosoma brucei

1975 • 917 citations

Excision of an intact intron as a novel lariat structure during pre-mRNA splicing in vitro

1984 • 761 citations

PRE-mRNA SPLICING

1986 • 673 citations

Cultivation in a semi-defined medium of animal infective forms of Trypanosoma brucei, T. equiperdum, T. evansi, T. rhodesiense and T. gambiense.

1985 • 567 citations

Detection of Single Base Substitutions by Ribonuclease Cleavage at Mismatches in RNA:DNA Duplexes

1985 • 538 citations

RNA splicing is interrupted by heat shock and is rescued by heat shock protein synthesis

1986 • 524 citations

Evidence for Trans splicing in trypanosomes

1986 • 486 citations

Lariat RNA's as Intermediates and Products in the Splicing of Messenger RNA precursors

1984 • 474 citations

DISCONTINUOUS TRANSCRIPTION AND ANTIGENIC VARIATION IN TRYPANOSOMES

1986 • 418 citations

Identification of a novel Y branch structure as an intermediate in trypanosome mRNA processing: Evidence for Trans splicing

1986 • 354 citations

Inactivation of transcription by UV irradiation of T. brucei provides evidence for a multicistronic transcription unit including a VSG gene

1987 • 307 citations

An RNA Processing Activity That Debranches RNA Lariats

1985 • 300 citations

The preferential translation of Drosophila hsp70 mRNA requires sequences in the untranslated leader

1985 • 291 citations

Tubulin genes are tandemly linked and clustered in the genome of trypanosoma brucei

1983 • 265 citations

Transcripts coding for variant surface glycoproteins of Trypanosoma brucei have a short, identical exon at their 5′ end

1982 • 243 citations

Apparent discontinuous transcription of Trypanosoma brucei variant surface antigen genes

1984 • 193 citations

RNA splicing is required to make the messenger RNA for a variant surface antigen in trypanosomes

1982 • 183 citations

Trypanosome mRNAs have unusual "cap 4" structures acquired by addition of a spliced leader.

1987 • 179 citations

Identification of a small rna containing the trypanosome spliced leader: A donor of shared 5′ sequences of trypanosomatid mRNAs?

1984 • 176 citations

Topogenesis of microbody enzymes: a sequence comparison of the genes for the glycosomal (microbody) and cytosolic phosphoglycerate kinases of Trypanosoma brucei.

1985 • 168 citations

The 35-Nucleotide Spliced Leader Sequence Is Common to All Trypanosome Messenger RNA's

1986 • 164 citations

Calmodulin genes in trypanosomes are tandemly repeated and produce multiple mRNAs with a common 5' leader sequence.

1985 • 162 citations

The α- and β-tubulins of Toxoplasma gondii are encoded by single copy genes containing multiple introns

1988 • 152 citations

Comparison of the genes coding for the common 5′ terminal sequence of messenger RNAs in three trypanosome species

1984 • 150 citations

Discontinuous synthesis of mRNA in trypanosomes.

1984 • 145 citations

Polygene transcripts are precursors to calmodulin mRNAs in trypanosomes.

1988 • 143 citations

Sequences homologous to the variant antigen mRNA spliced leader are located in tandem repeats and variable orphons in Trypanosoma brucei

1983 • 143 citations

Developmental Regulation of a Novel Repetitive Protein of Trypanosoma brucei

1987 • 140 citations

Tubulin genes of the African trypanosome Trypanosoma brucei rhodesiense: nucleotide sequence of a 3.7-kb fragment containing genes for alpha and beta tubulins

1985 • 135 citations

Characterization of the gene for the microbody (glycosomal) triosephosphate isomerase of Trypanosoma brucei.

1986 • 135 citations

Two hybrid plasmids with D. melanogaster DNA sequences complementary to mRNA coding for the major heat shock protein

1978 • 134 citations

Two tandemly linked identical genes code for the glycosomal glyceraldehyde-phosphate dehydrogenase in Trypanosoma brucei.

1986 • 130 citations

ANTIGENIC VARIATION IN AFRICAN TRYPANOSOMES

1985 • 121 citations

Precise identification of cleavage sites involved in the unusual processing of trypanosome ribosomal RNA

1987 • 117 citations

Structure and regulated expression of genes encoding fructose biphosphate aldolase in Trypanosoma brucei.

1985 • 110 citations

Tandem arrangement of tubulin genes in the protozoan parasite Leishmania enriettii.

1983 • 109 citations

Conserved Sequences and Transcription of the hsp70 Gene Family in Trypanosoma brucei

1986 • 108 citations

Apparent generation of a segmented mRNA from two separate tandem gene families inTrypanosoma cruzi

1985 • 106 citations

Tubulin genes of Trypanosoma brucei: a tightly clustered family of alternating genes.

1983 • 100 citations

Cloning and characterization of a Leishmania gene encoding a RNA spliced leader sequence

1986 • 99 citations

Cysteine eliminates the feeder cell requirement for cultivation of Trypanosoma brucei bloodstream forms in vitro.

1985 • 97 citations

Mature mRNAs ofTrypanosoma bruceipossess a 5′ cap acquired by discontinuous RNA synthesis

1985 • 95 citations

Changes in the half-life of ribosomal protein messenger RNA caused by translational repression

1986 • 92 citations

In vivo labelling of intermediates in the discontinuous synthesis of mRNAs in Trypanosoma brucei.

1987 • 92 citations

Isopycnic isolation of African trypanosomes on Percoll gradients formed in situ.

1982 • 92 citations

The common 5′ terminal sequence on trypanosome mRNAs: a target for anti-messenger oligodeoxynucleotides

1986 • 91 citations

RNA turnover in Trypanosoma brucei.

1987 • 89 citations

A 5' spliced leader is added in trans to both alpha- and beta-tubulin transcripts in Trypanosoma brucei.

1985 • 88 citations

Transcription of the intergenic regions of the tubulin gene cluster ofTrypanosoma brucei: evidence for a polyclstronic transcription unit in a eukaryote

1987 • 79 citations

Direct analysis of the mini-exon donor RNA ofTrypanosoma brucei: detection of a novel cap structure also present in messenger RNA

1987 • 73 citations

The monogenetic kinetoplastid protozoan,Crithidia fasciculate, contains a transcriptionally active, multicopy mini-exon sequence

1987 • 64 citations

Purine salvage by Tritrichomonas foetus

1983 • 59 citations

Tubulin mRNAs of Trypanosoma brucei

1986 • 45 citations

Comparison of maxicircle DNAs of Leishmania tarentolae and Trypanosoma brucei.

1983 • 45 citations

Trypanosome trans-splicing utilizes 2′-5′ branches and a corresponding debranching activity.

1988 • 37 citations

The cap of both miniexon-derived RNA and mRNA of trypanosomes is 7-methylguanosine.

1988 • 28 citations