Domain structure and interactions of recombinant urokinase-type plasminogen activator.

The Anatomy and Taxonomy of Protein Structure

1981 • 3,376 citations

Why genes in pieces?

1978 • 2,256 citations

Stability of Proteins Small Globular Proteins

1979 • 2,139 citations

Kinetics of the activation of plasminogen by human tissue plasminogen activator. Role of fibrin.

1982 • 1,300 citations

Cold Denaturation of Protein

1990 • 1,023 citations

The refined 1.9 A crystal structure of human alpha-thrombin: interaction with D-Phe-Pro-Arg chloromethylketone and significance of the Tyr-Pro-Pro-Trp insertion segment.

1989 • 889 citations

[2]Scanning microcalorimetry in studying temperature-induced changes in proteins

1986 • 755 citations

Structure of crystalline α-chymotrypsin

1972 • 675 citations

Evolution of the proteases of blood coagulation and fibrinolysis by assembly from modules

1985 • 492 citations

The receptor-binding sequence of urokinase. A biological function for the growth-factor module of proteases.

1987 • 479 citations

Differentiation-enhanced binding of the amino-terminal fragment of human urokinase plasminogen activator to a specific receptor on U937 monocytes.

1985 • 450 citations

Statistical mechanical deconvolution of thermal transitions in macromolecules. I. Theory and application to homogeneous systems

1978 • 349 citations

Structure and function of epidermal growth factor‐like regions in proteins

1988 • 305 citations

EGF‐like domains in extracellular matrix proteins: Localized signals for growth and differentiation?

1989 • 304 citations

Do genes-in-pieces imply proteins-in-pieces?

1978 • 287 citations

The role of beta-hydroxyaspartate and adjacent carboxylate residues in the first EGF domain of human factor IX.

1988 • 240 citations

One-chain urokinase-type plasminogen activator from human sarcoma cells is a proenzyme with little or no intrinsic activity.

1988 • 237 citations

Thermodynamics of protein denaturation. Calorimetric study of the reversible denaturation of chymotrypsinogen and conclusions regarding the accuracy of the two-state approximation

1970 • 224 citations

Purification of urokinase by affinity chromatography

1976 • 207 citations

The atomic structure of crystalline porcine pancreatic elastase at 2.5 Å resolution: Comparisons with the structure of α-chymotrypsin

1978 • 165 citations

Biochemical and biological aspects of the plasminogen activation system

1990 • 165 citations

The Crystal and Molecular Structure of DIP-inhibited Bovine Trypsin at 2.7A Resolution

1972 • 147 citations

Thermodynamic Characterization of Conformational States of Biological Macromolecules using Differential Scanning Calorimetry

1978 • 145 citations

Purification and characterization of a novel low molecular weight form of single-chain urokinase-type plasminogen activator.

1986 • 114 citations

Domains in human plasminogen

1984 • 107 citations

Characterization of a fusion protein consisting of amino acids 1 to 263 of tissue-type plasminogen activator and amino acids 144 to 411 of urokinase-type plasminogen activator.

1987 • 89 citations

The existence of independent domain structures in human Lys77-plasminogen.

1981 • 89 citations

Domain structure and interactions of the type I and type II modules in the gelatin-binding region of fibronectin

1991 • 53 citations

Domain structure and domain-domain interactions of recombinant tissue plasminogen activator

1991 • 51 citations

Fibrinolytic properties of single chain urokinase-type plasminogen activator (Pro-urokinase)

1990 • 49 citations

Artificial exon shuffling between tissue-type plasminogen activator (t-PA) and urokinase (u-PA): a comparative study on the fibrinolytic properties of t-PA/u-PA hybrid proteins

1988 • 47 citations

The folding of pancreatic elastase: Independent domain refolding and inter-domain interaction

1978 • 47 citations

Single-chain urokinase-type plasminogen activator does not possess measurable intrinsic amidolytic or plasminogen activator activities

1991 • 45 citations

Reversible independent unfolding of the domains of urokinase monitored by proton NMR

1989 • 42 citations

Calorimetric evaluation of the existence of separate domains in bovine prothrombin

1981 • 42 citations

Characterization of a recombinant fusion protein of the finger domain of tissue-type plasminogen activator with a truncated single chain urokinase-type plasminogen activator.

1987 • 41 citations

Evolutionary Assembly of Blood Coagulation Proteins

1990 • 40 citations

Single-Chain Urokinase-Type Plasminogen Activator: Mechanism of Action and Thrombolytic Properties

1987 • 40 citations

On the conversion of high molecular weight urokinase to the low molecular weight form by plasmin

1981 • 40 citations

Construction and expression of hybrid plasminogen activators prepared from tissue-type plasminogen activator and urokinase-type plasminogen activator genes.

1988 • 39 citations

Mutant and chimeric recombinant plasminogen activators. Production in eukaryotic cells and preliminary characterization.

1987 • 37 citations

Conformation of Lys-plasminogen and the kringle 1-3 fragment of plasminogen analyzed by small-angle neutron scattering

1991 • 35 citations

Characterization of a chimeric plasminogen activator consisting of amino acids 1 to 274 of tissue-type plasminogen activator and amino acids 138 to 411 of single-chain urokinase-type plasminogen activator.

1988 • 33 citations

High level expression of human proteins in murine hybridoma cells: induction by methotrexate in the absence of gene amplification

1991 • 29 citations

Effect of residue 65 substitutions on thermal stability of tissue plasminogen activator kringle-2 domain

1989 • 28 citations

Nuclear‐magnetic‐resonance studies of human epidermal growth factor

1990 • 20 citations

Independent refolding of domains in the pancreatic serine proteinases.

1986 • 19 citations

Conformation of one- and two-chain high molecular weight urokinase analyzed by small-angle neutron scattering and vacuum ultraviolet circular dichroism

1991 • 15 citations

Characterization of a chimaeric plasminogen activator obtained by insertion of the second kringle structure of tissue-type plasminogen activator (amino acids 173 through 262) between residues Asp130 and Ser139 of urokinase-type plasminogen activator

1988 • 12 citations