Mechanism of activation of protein kinase B by insulin and IGF-1.

A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye Binding

1976 • 225,752 citations

Site-directed mutagenesis by overlap extension using the polymerase chain reaction

1989 • 7,496 citations

Inhibition of glycogen synthase kinase-3 by insulin mediated by protein kinase B

1995 • 5,143 citations

Protein kinase B (c-Akt) in phosphatidylinositol-3-OH kinase signal transduction

1995 • 2,106 citations

SB 203580 is a specific inhibitor of a MAP kinase homologue which is stimulated by cellular stresses and interleukin‐1

1995 • 2,080 citations

The protein kinase encoded by the Akt proto-oncogene is a target of the PDGF-activated phosphatidylinositol 3-kinase

1995 • 2,024 citations

A novel kinase cascade triggered by stress and heat shock that stimulates MAPKAP kinase-2 and phosphorylation of the small heat shock proteins

1994 • 1,684 citations

Cloning, structure, and expression of the mitochondrial cytochrome P-450 sterol 26-hydroxylase, a bile acid biosynthetic enzyme

1989 • 1,244 citations

Molecular cloning of the CD2 antigen, the T-cell erythrocyte receptor, by a rapid immunoselection procedure.

1987 • 949 citations

Calcium phosphate-mediated gene transfer: a highly efficient transfection system for stably transforming cells with plasmid DNA.

1988 • 897 citations

Amplification of AKT2 in human pancreatic cells and inhibition of AKT2 expression and tumorigenicity by antisense RNA.

1996 • 785 citations

AKT2, a putative oncogene encoding a member of a subfamily of protein-serine/threonine kinases, is amplified in human ovarian carcinomas.

1992 • 732 citations

Specific and high-affinity binding of inositol phosphates to an isolated pleckstrin homology domain.

1995 • 569 citations

Molecular cloning and identification of a serine/threonine protein kinase of the second-messenger subfamily.

1991 • 537 citations

Activation and phosphorylation of a pleckstrin homology domain containing protein kinase (RAC-PK/PKB) promoted by serum and protein phosphatase inhibitors.

1996 • 490 citations

Molecular cloning and characterisation of a novel putative protein‐serine kinase related to the cAMP‐dependent and protein kinase C families

1991 • 485 citations

PI 3-kinase is a dual specificity enzyme: autoregulation by an intrinsic protein-serine kinase activity.

1994 • 450 citations

Membrane Localization of Phosphatidylinositol 3-Kinase Is Sufficient To Activate Multiple Signal-Transducing Kinase Pathways

1996 • 443 citations

The principal target of rapamycin-induced p70s6k inactivation is a novel phosphorylation site within a conserved hydrophobic domain.

1995 • 441 citations

Insulin stimulates the kinase activity of RAC-PK, a pleckstrin homology domain containing ser/thr kinase.

1995 • 358 citations

Specific binding of the Akt-1 protein kinase to phosphatidylinositol 3,4,5-trisphosphate without subsequent activation

1996 • 328 citations

Structure of the binding site for inositol phosphates in a PH domain.

1995 • 281 citations

Isolation of transforming murine leukemia viruses from mice with a high incidence of spontaneous lymphoma.

1977 • 273 citations

The phosphatidylinositol 3-kinase serine kinase phosphorylates IRS-1. Stimulation by insulin and inhibition by Wortmannin.

1994 • 234 citations

Cellular mechanism of metformin action involves glucose transporter translocation from an intracellular pool to the plasma membrane in L6 muscle cells.

1992 • 227 citations

Wortmannin inhibits the effects of insulin and serum on the activities of glycogen synthase kinase-3 and mitogen-activated protein kinase

1994 • 204 citations

Molecular Cloning and Characterization of a New Member of the RAC Protein Kinase Family: Association of the Pleckstrin Homology Domain of 3 Types of RAC Protein Kinase with Protein Kinase C Subspecies and βγ Subunits of G Proteins

1995 • 189 citations

Identification of novel phosphorylation sites required for activation of MAPKAP kinase-2.

1995 • 187 citations

Constitutive activation of protein kinase B and phosphorylation of p47 phox by a membrane-targeted phosphoinositide 3-kinase

1996 • 130 citations

Determination of in Vivo Phosphorylation Sites in Protein Kinase C

1995 • 102 citations

Developmental Regulation of Expression and Activity of Multiple Forms of the Drosophila RAC Protein Kinase

1995 • 58 citations