Phosphorylation of eukaryotic initiation factor 4E (eIF4E) at Ser209 is not required for protein synthesis in vitro and in vivo

Designer deletion strains derived fromSaccharomyces cerevisiae S288C: A useful set of strains and plasmids for PCR-mediated gene disruption and other applications

1998 • 3,373 citations

Improved method for high efficiency transformation of intact yeast cells

1992 • 3,258 citations

New heterologous modules for classical or PCR‐based gene disruptions in Saccharomyces cerevisiae

1994 • 2,554 citations

eIF4 Initiation Factors: Effectors of mRNA Recruitment to Ribosomes and Regulators of Translation

1999 • 2,096 citations

Yeast vectors for the controlled expression of heterologous proteins in different genetic backgrounds

1995 • 1,943 citations

Regulation of 4E-BP1 phosphorylation: a novel two-step mechanism

1999 • 1,255 citations

Translational control of gene expression

2001 • 1,192 citations

Regulatable promoters of Saccharomyces cerevisiae: comparison of transcriptional activity and their use for heterologous expression

1994 • 968 citations

Initiation of Protein Synthesis in Eukaryotic Cells

1996 • 715 citations

The Translation Initiation Factor eIF-4E Binds to a Common Motif Shared by the Translation Factor eIF-4γ and the Translational Repressors 4E-Binding Proteins

1995 • 662 citations

Cocrystal Structure of the Messenger RNA 5′ Cap-Binding Protein (eIF4E) Bound to 7-methyl-GDP

1997 • 660 citations

Human eukaryotic translation initiation factor 4G (eIF4G) recruits Mnk1 to phosphorylate eIF4E

1999 • 635 citations

Repression of cap-dependent translation by 4E-binding protein 1: competition with p220 for binding to eukaryotic initiation factor-4E.

1995 • 606 citations

[4] Preparation and use of nuclease-treated rabbit reticulocyte lysates for the translation of eukaryotic messenger RNA

1983 • 547 citations

Mapping of Functional Domains in Eukaryotic Protein Synthesis Initiation Factor 4G (eIF4G) with Picornaviral Proteases

1995 • 541 citations

The role of the 5′ untranslated region of an mRNA in translation regulation during development

1999 • 369 citations

The Phosphorylation of Eukaryotic Initiation Factor eIF4E in Response to Phorbol Esters, Cell Stresses, and Cytokines Is Mediated by Distinct MAP Kinase Pathways

1998 • 302 citations

Human Eukaryotic Translation Initiation Factor 4G (eIF4G) Possesses Two Separate and Independent Binding Sites for eIF4A

1997 • 289 citations

Chromatographic resolution of in vivo phosphorylated and nonphosphorylated eukaryotic translation initiation factor eIF-4E: increased cap affinity of the phosphorylated form.

1994 • 285 citations

15 Translational Control of Developmental Decisions

2000 • 222 citations

Phosphorylation of Eukaryotic Protein Synthesis Initiation Factor 4E at Ser-209

1995 • 210 citations

The C-terminal domain of eukaryotic protein synthesis initiation factor (eIF) 4G is sufficient to support cap-independent translation in the absence of eIF4E.

1996 • 207 citations

Foot-and-mouth disease virus leader proteinase: purification of the Lb form and determination of its cleavage site on eIF-4 gamma

1994 • 191 citations

Upregulation of protein synthesis initiation factor eIF-4E is an early event during colon carcinogenesis

1999 • 175 citations

eIF4G: translation's mystery factor begins to yield its secrets.

1997 • 163 citations

The eIF4G-eIF4E complex is the target for direct cleavage by the rhinovirus 2A proteinase

1996 • 155 citations

Serine 209, Not Serine 53, Is the Major Site of Phosphorylation in Initiation Factor eIF-4E in Serum-treated Chinese Hamster Ovary Cells

1995 • 149 citations

A Reevaluation of the Cap-binding Protein, eIF4E, as a Rate-limiting Factor for Initiation of Translation in Reticulocyte Lysate

1996 • 139 citations

Amino acid sequence of the human protein synthesis initiation factor eIF-4 gamma

1992 • 139 citations

Alteration of the major phosphorylation site of eukaryotic protein synthesis initiation factor 4E prevents its association with the 48 S initiation complex.

1990 • 133 citations

Translational enhancement of FGF-2 by eIF-4 factors, and alternate utilization of CUG and AUG codons for translation initiation.

1995 • 131 citations

Involvement of Stress-activated Protein Kinase and p38/RK Mitogen-activated Protein Kinase Signaling Pathways in the Enhanced Phosphorylation of Initiation Factor 4E in NIH 3T3 Cells

1997 • 127 citations

A Mammalian Translation Initiation Factor Can Substitute for Its Yeast Homologue in Vivo

1989 • 114 citations

Cap binding protein complex that restores protein synthesis in heat-shocked Ehrlich cell lysates contains highly phosphorylated eIF-4E.

1990 • 110 citations

Amino acid-induced stimulation of translation initiation in rat skeletal muscle

1999 • 106 citations

Eukaryotic mRNAs encoding abundant and scarce proteins are statistically dissimilar in many structural features

1998 • 101 citations

Dissociation of the eukaryotic initiation factor‐4E/4E‐BP1 complex involves phosphorylation of 4E‐BP1 by an mTOR‐associated kinase

1999 • 97 citations

Modulation of Translation Initiation in Rat Skeletal Muscle and Liver in Response to Food Intake

1997 • 88 citations

Pim-1 protein expression is regulated by its 5'-untranslated region and translation initiation factor elF-4E.

1997 • 87 citations

Regulation of ornithine decarboxylase in a transformed cell line that overexpresses translation initiation factor eIF-4E.

1996 • 72 citations

Caspase‐3 is necessary and sufficient for cleavage of protein synthesis eukaryotic initiation factor 4G during apoptosis

1999 • 72 citations

Translation initiation factor 4E

1999 • 70 citations

The Association of Initiation Factor 4F with Poly(A)-binding Protein Is Enhanced in Serum-stimulated Xenopus Kidney Cells

1999 • 64 citations

15 PERK and Translational Control by Stress in the Endoplasmic Reticulum

2000 • 57 citations

The proteolytic cleavage of eukaryotic initiation factor (eIF) 4G is prevented by eIF4E binding protein (PHAS-I; 4E-BP1) in the reticulocyte lysate

1997 • 53 citations

Modifications of eukaryotic initiation factor 4F (eIF4F) in adult cardiocytes by adenoviral gene transfer: differential effects on eIF4F activity and total protein synthesis rates

2001 • 40 citations

The role of 5'-leader length, secondary structure and PABP concentration on cap and poly(A) tail function during translation in Xenopus oocytes

2000 • 39 citations

Secondary structure in the 5′‐leader or 3′‐untranslated region reduces protein yield but does not affect the functional interaction between the 5′‐cap and the poly(A) tail

1999 • 37 citations

Protein phosphorylation in cell growth regulation

1996 • 36 citations

Use of Nonreducing SDS-PAGE for Monitoring Renaturation of Recombinant Protein Synthesis Initiation Factor, eIF-4α

1993 • 33 citations

Cellular stress in Xenopus kidney cells enhances the phosphorylation of eukaryotic translation initiation factor (eIF)4E and the association of eIF4F with poly(A)-binding protein

1999 • 30 citations

Modifications of eukaryotic initiation factor 4F (eIF4F) in adult cardiocytes by adenoviral gene transfer: differential effects on eIF4F activity and total protein synthesis rates

2001 • 26 citations

Prediction of eukaryotic mRNA translational properties.

1999 • 24 citations

Effect of mRNA Cap Structure on eIF-4E Phosphorylation and Cap Binding Analyses Using Ser209-Mutated eIF-4Es

1998 • 21 citations

A fractionated reticulocyte lysate retains high efficiency for protein synthesis

1990 • 20 citations

Role of Ser-53 phosphorylation in the activity of human translation initiation factor eIF-4E in mammalian and yeast cells

1995 • 11 citations

16 Regulation of Translation Initiation in Mammalian Cells by Amino Acids

2000 • 7 citations

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