Kinetic analysis of ouabain binding to native and mutated forms of Na,K-ATPase and identification of a new region involved in cardiac glycoside interactions.

Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4

1970 • 253,849 citations

A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye Binding

1976 • 225,752 citations

A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding

1976 • 200,127 citations

Single-Step Method of RNA Isolation by Acid Guanidinium Thiocyanate–Phenol–Chloroform Extraction

1987 • 64,270 citations

Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.

1979 • 54,907 citations

Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction

1987 • 45,673 citations

Genomic sequencing.

1984 • 8,343 citations

High-efficiency transformation of mammalian cells by plasmid DNA.

1987 • 5,377 citations

Analysis of single- and double-stranded nucleic acids on polyacrylamide and agarose gels by using glyoxal and acridine orange.

1977 • 2,408 citations

The sodium-potassium adenosine triphosphatase: pharmacological, physiological and biochemical aspects.

1975 • 868 citations

Electrogenic sodium pump in nerve and muscle cells.

1972 • 787 citations

Regulation of cellular volume.

1977 • 607 citations

Molecular Genetics of Na,K-ATPase

1990 • 411 citations

Structure-function relationships in the sodium-potassium ATPase .alpha. subunit: site-directed mutagenesis of glutamine-111 to arginine and asparagine-122 to aspartic acid generates a ouabain-resistant enzyme

1988 • 403 citations

The role of Na+,K+-ATPase in the inotropic action of digitalis.

1977 • 335 citations

Comparison of the substrate dependence properties of the rat Na,K-ATPase alpha 1, alpha 2, and alpha 3 isoforms expressed in HeLa cells.

1991 • 308 citations

A General Method for Saturation Mutagenesis of Cloned DNA Fragments

1985 • 292 citations

Characterization of a new photoaffinity derivative of ouabain: labeling of the large polypeptide and of a proteolipid component of the (sodium-potassium ion)-dependent ATPase

1978 • 282 citations

Possible involvement of cardiac Na+, K+-adenosine triphosphatase in the mechanism of action of cardiac glycosides.

1969 • 254 citations

Purification and Characterization of and [3H]Ouabain Binding to the Transport Adenosine Triphosphatase from Outer Medulla of Canine Kidney

1973 • 210 citations

Sugar, Amino Acid, and NA+ Cotransport in the Proximal Tubule

1979 • 191 citations

Interaction of cardiac glycosides with (Na+ + K+)-activated ATPase. A biochemical link to digitalis-induced inotropy.

1984 • 186 citations

Photoaffinity Labeling of the Ouabain-Binding Site on (Na ⁺ + K ⁺ ) Adenosinetriphosphatase

1974 • 160 citations

Primary sequence and functional expression of a novel ouabain-resistant Na,K-ATPase. The beta subunit modulates potassium activation of the Na,K-pump.

1992 • 159 citations

Structure-function studies of Na,K-ATPase. Site-directed mutagenesis of the border residues from the H1-H2 extracellular domain of the alpha subunit.

1990 • 159 citations

Molecular basis for the insensitivity of the Monarch (Danaus plexippus) to cardiac glycosides

1992 • 127 citations

Mutation of a cysteine in the first transmembrane segment of Na,K-ATPase alpha subunit confers ouabain resistance.

1992 • 109 citations

Cardiotonic Steroid Binding to Na,K-ATPase

1983 • 105 citations

Evidence for the organization of the transmembrane segments of (Na,K)-ATPase based on labeling lipid-embedded and surface domains of the alpha-subunit.

1982 • 88 citations

Site-directed Mutagenesis of a Conserved, Extracellular Aspartic Acid Residue Affects the Ouabain Sensitivity of Sheep Na,K-ATPase

1989 • 86 citations

A kinetic comparison of cardiac glycoside interactions with Na+,K+-ATPases from skeletal and cardiac muscle and from kidney

1980 • 80 citations

Location of Asn831 of the alpha chain of Na/K-ATPase at the cytoplasmic surface. Implication for topological models.

1993 • 71 citations

The epitope for monoclonal antibody A20 (amino acids 870-890) is located on the luminal surface of the Ca2(+)-ATPase of sarcoplasmic reticulum.

1990 • 71 citations

[19] Interaction of cardiac glycosides with Na+,K+-ATPase

1988 • 65 citations

Digitalis: Its Mode of Action, Receptor, and Structure–Activity Relationships

1990 • 64 citations

Transmembranous organization of (Ca2(+)-Mg2+)-ATPase from sarcoplasmic reticulum. Evidence for lumenal location of residues 877-888.

1990 • 62 citations

Photoaffinity labeling of the digitalis receptor in the (Sodium + Potassium) - activated adenosinetriphosphatase

1979 • 62 citations

Substitution of transmembrane residues with hydrogen-bonding potential in the .alpha. subunit of sodium-potassium ATPase reveals alterations in ouabain sensitivity

1993 • 60 citations

Na,K-ATPase extracellular surface probed with a monoclonal antibody that enhances ouabain binding.

1992 • 59 citations

Affinity labeling of the digitalis receptor with p-nitrophenyltriazene-ouabain, a highly specific alkylating agent.

1980 • 58 citations

Ouabain-binding-site photoaffinity probes that label both subunits of Na+,K+-ATPase.

1980 • 57 citations

ASSOCIATION AND DISSOCIATION RATE CONSTANTS OF THE COMPLEXES BETWEEN VARIOUS CARDIAC MONOGLYCOSIDES AND N<scp>a</scp>, K‐ATP<scp>ase</scp>*

1974 • 52 citations

Functional properties of an H,K-ATPase/Na,K-ATPase chimera

1993 • 51 citations

Isolation and characterization of monoclonal antibodies to (Na+ + K+)-ATPase

1982 • 46 citations

Specific photoaffinity labeling of the digitalis binding site of the sodium and potassium ion-activated adenosine triphosphatase induced by energy transfer

1983 • 39 citations

Kinetic studies on muscarinic antagonist-agonist competition.

1980 • 31 citations

Amino acid residues of the Na,K-ATPase involved in ouabain sensitivity do not bind the sugar moiety of cardiac glycosides.

1993 • 30 citations

Molecular dissection of functional domains of the E1E2-ATPase using sodium and calcium pump chimeric molecules

1992 • 27 citations

Rate constants of agonist binding to muscarinic receptors in rat brain medulla. Evaluation by competition kinetics.

1985 • 25 citations

Canine Mesenteric Artery Na+,K+-ATPase

1983 • 23 citations

Analysis of ligand binding to receptors by competition kinetics. Application to muscarinic antagonists in rat brain cortex.

1985 • 22 citations

Identification of cysteine residues in lamb kidney (Na,K)-ATPase essential for ouabain binding.

1991 • 22 citations

Structure-activity relationships of cardiotonic steroids for the inhibition of sodium- and potassium-dependent adenosine triphosphatase. V. Dissociation rate constants of digitoxin acetates.

1975 • 18 citations

Photoaffinity labeling of the sodium- and potassium-activated adenosinetriphosphatase with a cardiac glycoside containing the photoactive group on the C-17 side chain

1983 • 13 citations

[9] Preparation of antibodies to Na+,K+-ATPase and its subunits

1988 • 9 citations