A General Method for Saturation Mutagenesis of Cloned DNA Fragments

DNA sequencing with chain-terminating inhibitors

1977 • 69,181 citations

[57] Sequencing end-labeled DNA with base-specific chemical cleavages

1980 • 15,302 citations

[2] New M13 vectors for cloning

1983 • 5,402 citations

Analysis of gene control signals by DNA fusion and cloning in Escherichia coli

1980 • 2,499 citations

pEMBL: a new family of single stranded plasmids

1983 • 1,231 citations

Hydrogen bonding and biological specificity analysed by protein engineering

1985 • 1,050 citations

DNA fragments differing by single base-pair substitutions are separated in denaturing gradient gels: correspondence with melting theory.

1983 • 895 citations

Multiple Point Mutations Affecting the Simian Virus 40 Enhancer

1983 • 883 citations

Nearly all single base substitutions in DNA fragments joined to a GC-clamp can be detected by denaturing gradient gel electrophoresis

1985 • 606 citations

Mutagenesis at a specific position in a DNA sequence.

1978 • 504 citations

Detection of single base substitutions in total genomic DNA

1985 • 375 citations

Length-independent separation of DNA restriction fragments in two-dimensional gel electrophoresis

1979 • 341 citations

Insertion of nucleotides opposite apurinic apyrimidinic sites in deoxyribonucleic acid during in vitro synthesis: uniqueness of adenine nucleotides

1983 • 320 citations

Local mutagenesis: a method for generating viral mutants with base substitutions in preselected regions of the viral genome.

1978 • 282 citations

Modification of the melting properties of duplex DNA by attachment of a GC-rich DNA sequence as determined by denaturing gradient gel electrophoresis

1985 • 252 citations

Oligonucleotide directed mutagenesis of the human β-globin gene: a general method for producing specific point mutations in cloned DNA

1981 • 207 citations

Efficient site-directed mutagenesis by simultaneous use of two primers

1983 • 154 citations

DIRECTED MUTAGENESIS

1981 • 142 citations

Depurination-induced infidelity of DNA synthesis with purified DNA replication proteins in vitro

1983 • 137 citations

Substituting an α-helix switches the sequence-specific DNA interactions of a repressor

1984 • 130 citations

On the fidelity of DNA replication. Lack of exodeoxyribonuclease activity and error-correcting function in avian myeloblastosis virus DNA polymerase.

1976 • 93 citations

Minimal size plasmids containing an M13 origin for production of single-strand transducing particles.

1984 • 82 citations

Gap misrepair mutagenesis: efficient site-directed induction of transition, transversion, and frameshift mutations in vitro.

1982 • 81 citations

Local mutagenesis within deletion loops of DNA heteroduplexes.

1982 • 67 citations

Mutagenesis in vitro by depurination of ΦX174 DNA

1981 • 59 citations

In vitro characterization of the fibroin gene promoter by the use of single-base substitution mutants.

1982 • 45 citations

[11] Two-dimensional electrophoretic separation of restriction enzyme fragments of DNA

1979 • 38 citations

Segment-specific mutagenesis: extensive mutagenesis of a lac promoter/operator element.

1982 • 35 citations

Site-specific mutagenesis by error-directed DNA synthesis

1982 • 35 citations

Development of an Enzyme-linked Immunosorbent Assay (ELISA) for hepatitis B e antigen and antibody

1981 • 23 citations