Gene-fusion techniques for determining membrane-protein topology

Membrane protein structure prediction

1992 • 1,680 citations

TnphoA: a transposon probe for protein export signals.

1985 • 1,030 citations

The spontaneous insertion of proteins into and across membranes: The helical hairpin hypothesis

1981 • 868 citations

The distribution of positively charged residues in bacterial inner membrane proteins correlates with the trans-membrane topology

1986 • 861 citations

A Genetic Approach to Analyzing Membrane Protein Topology

1986 • 610 citations

Carboxy-terminal phenylalanine is essential for the correct assembly of a bacterial outer membrane protein

1991 • 483 citations

Determinants of membrane protein topology.

1987 • 301 citations

Enhancement of membrane insertion and function in a type IIIb membrane protein following introduction of a cleavable signal peptide.

1992 • 260 citations

Alkaline phosphatase fusions: sensors of subcellular location

1990 • 254 citations

lac permease of Escherichia coli: topology and sequence elements promoting membrane insertion.

1990 • 248 citations

Topology and functional domains of Sec63p, an endoplasmic reticulum membrane protein required for secretory protein translocation.

1992 • 239 citations

Fusions of secreted proteins to alkaline phosphatase: an approach for studying protein secretion.

1985 • 238 citations

Chapter 3 Analysis of Membrane Protein Topology Using Alkaline Phosphatase and β-Galactosidase Gene Fusions

1991 • 231 citations

Bacterial Motility: Membrane Topology of the Escherichia coli MotB Protein

1988 • 228 citations

Topology analysis of the SecY protein, an integral membrane protein involved in protein export in Escherichia coli.

1987 • 223 citations

Penicillin-Binding Proteins of Gram-Negative Bacteria

1988 • 212 citations

Escherichia coli alkaline phosphatase fails to acquire disulfide bonds when retained in the cytoplasm

1991 • 204 citations

Probing the topology of a bacterial membrane protein by genetic insertion of a foreign epitope; expression at the cell surface.

1986 • 193 citations

Genetic analysis of the membrane insertion and topology of MalF, a cytoplasmic membrane protein of Escherichia coli

1988 • 175 citations

Role of primary structure and disulfide bond formation in beta-lactamase secretion

1983 • 170 citations

A signal sequence is not sufficient to lead β-galactosidase out of the cytoplasm

1980 • 149 citations

Use of gene fusions to study outer membrane protein localization in Escherichia coli.

1977 • 148 citations

Analysis of protein localization by use of gene fusions with complementary properties

1990 • 147 citations

A vector for the construction of translational fusions to TEM β-lactamase and the analysis of protein export signals and membrane protein topology

1986 • 146 citations

Membrane topology of the ArsB protein, the membrane subunit of an anion-translocating ATPase.

1992 • 146 citations

Genetic analysis of membrane protein topology by a sandwich gene fusion approach.

1990 • 138 citations

Analysis of the membrane organization of an Escherichia coli protein translocator, HlyB, a member of a large family of prokaryote and eukaryote surface transport proteins

1991 • 132 citations

β‐lactamase as a probe of membrane protein assembly and protein export

1990 • 128 citations

Membrane topology of the Escherichia coli ExbD protein

1992 • 125 citations

Topology of the membrane-bound alkane hydroxylase of Pseudomonas oleovorans.

1992 • 112 citations

Use of phoA fusions to study the topology of the Escherichia coli inner membrane protein leader peptidase

1989 • 111 citations

The use of gene fusions to determine the topology of all of the subunits of the cytochrome o terminal oxidase complex of Escherichia coli.

1990 • 111 citations

Analysis of the topology of a membrane protein by using a minimum number of alkaline phosphatase fusions

1993 • 92 citations

Insertion derivatives containing segments of up to 16 amino acids identify surface- and periplasm-exposed regions of the FhuA outer membrane receptor of Escherichia coli K-12

1993 • 90 citations

Permissive sites and topology of an outer membrane protein with a reporter epitope

1991 • 86 citations

Membrane topology analysis of Escherichia coli mannitol permease by using a nested-deletion method to create mtlA-phoA fusions.

1991 • 81 citations

Membrane topology of the melibiose carrier of Escherichia coli.

1992 • 80 citations

Structural and functional dissection of a membrane glycoprotein required for vesicle budding from the endoplasmic reticulum.

1991 • 76 citations

Outer-membrane PhoE protein of Escherichia coli K-12 as an exposure vector: possibilities and limitations

1990 • 74 citations

[9] Genetic fusions as experimental tools

1991 • 71 citations

Genetic and biochemical evaluation of eucaryotic membrane protein topology: multiple transmembrane domains of Saccharomyces cerevisiae 3-hydroxy-3-methylglutaryl coenzyme A reductase.

1990 • 71 citations

Membrane protein spanning segments as export signals

1992 • 69 citations

Membrane topology of MaIG, an inner membrane protein from the maltose transport system of Escherichia coli

1993 • 61 citations

A topological analysis of subunit alpha from Escherichia coli F1F0-ATP synthase predicts eight transmembrane segments.

1990 • 59 citations

Use of a β‐lactamase fusion vector to investigate the organization of penicillin‐binding protein 1B in the cytoplasmic membrane of Escherichia coli

1987 • 52 citations

The role of the carboxy-terminal membrane-spanning fragment in the biogenesis of Escherichia coli K12 outer membrane protein PhoE

1989 • 52 citations

Topology of PhoE porin: the ‘eyelet’ region

1993 • 48 citations

In vivo topological analysis of Ste2, a yeast plasma membrane protein, by using beta-lactamase gene fusions.

1991 • 44 citations

The topology of the anchor subunit of dimethyl sulfoxide reductase of Escherichia coli.

1993 • 43 citations

Correct insertion of a simple eukaryotic plasma-membrane protein into the cytoplasmic membrane of Escherichia coli

1990 • 43 citations

An artificial hydrophobic sequence functions as either an anchor or a signal sequence at only one of two positions within the Escherichia coli outer membrane protein OmpA.

1988 • 38 citations

Use of cloned mtl genes of Escherichia coli to introduce mtl deletion mutations into the chromosome

1983 • 37 citations

The dynamics of assembly of a cytoplasmic membrane protein in Escherichia coli.

1992 • 36 citations

Export of FepA::PhoA fusion proteins to the outer membrane of Escherichia coli K-12

1989 • 31 citations

Dihydrofolate reductase (mouse) and beta-galactosidase (Escherichia coli) can be translocated across the plasma membrane of E. coli.

1988 • 29 citations

The use of gene-fusions to determine membrane protein topology in Saccharomyces cerevisiae

1989 • 22 citations

Analysis of the topology of the cytochrome d terminal oxidase complex of Escherichia coli by alkaline phosphatase fusions

1991 • 18 citations

Vectorial transport of proteins into and across membranes

1991 • 7 citations

β-Lactamase fusion analysis of membrane protein assembly

1992 • 7 citations