Structural and functional dissection of a membrane glycoprotein required for vesicle budding from the endoplasmic reticulum.

Molecular Cloning: A Laboratory Manual

2001 • 133,517 citations

DNA sequencing with chain-terminating inhibitors

1977 • 69,181 citations

Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.

1979 • 54,907 citations

Molecular Cloning. A Laboratory Manual

1983 • 27,328 citations

A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae.

1989 • 8,705 citations

Current protocols in molecular biology

1988 • 7,584 citations

Personal communication

1989 • 2,984 citations

[1] Production of single-stranded plasmid DNA

1987 • 2,642 citations

Identification of 23 complementation groups required for post-translational events in the yeast secretory pathway

1980 • 1,808 citations

All ras proteins are polyisoprenylated but only some are palmitoylated

1989 • 1,806 citations

Functional inactivation of genes by dominant negative mutations

1987 • 1,174 citations

Transformation in yeast: Development of a hybrid cloning vector and isolation of the can1 gene

1979 • 1,080 citations

Sequences That Regulate the Divergent GAL1-GAL10 Promoter in Saccharomyces cerevisiae

1984 • 955 citations

Order of events in the yeast secretory pathway

1981 • 804 citations

Distinct sets of SEC genes govern transport vesicle formation and fusion early in the secretory pathway

1990 • 721 citations

The yeast GTP-binding YPT1 protein and a mammalian counterpart are associated with the secretion machinery

1988 • 699 citations

hsp82 is an essential protein that is required in higher concentrations for growth of cells at higher temperatures.

1989 • 624 citations

Predicting the orientation of eukaryotic membrane-spanning proteins.

1989 • 596 citations

Early stages in the yeast secretory pathway are required for transport of carboxypeptidase Y to the vacuole

1982 • 579 citations

A novel GTP-binding protein, Sar1p, is involved in transport from the endoplasmic reticulum to the Golgi apparatus.

1989 • 443 citations

PROTEIN GLYCOSYLATION IN YEAST

1987 • 437 citations

Isolation of yeast mutants defective in protein targeting to the vacuole.

1986 • 394 citations

The Human Rab Genes Encode a Family of GTP-binding Proteins Related to Yeast YPT1 and SEC4 Products Involved in Secretion

1989 • 387 citations

Identification and structure of four yeast genes (SLY) that are able to suppress the functional loss of YPT1, a member of the RAS superfamily.

1991 • 347 citations

Reconstitution of SEC gene product-dependent intercompartmental protein transport

1988 • 332 citations

Mutational analysis of SEC4 suggests a cyclical mechanism for the regulation of vesicular traffic.

1989 • 314 citations

Distinct sequence determinants direct intracellular sorting and modification of a yeast vacuolar protease

1987 • 312 citations

Lyticase: Endoglucanase and Protease Activities That Act Together in Yeast Cell Lysis

1980 • 310 citations

Determinants of membrane protein topology.

1987 • 301 citations

Regulation of reversible binding of smg p25A, a ras p21-like GTP-binding protein, to synaptic plasma membranes and vesicles by its specific regulatory protein, GDP dissociation inhibitor.

1990 • 268 citations

The amino terminus of the yeast F1-ATPase beta-subunit precursor functions as a mitochondrial import signal.

1986 • 258 citations

Alkaline phosphatase fusions: sensors of subcellular location

1990 • 254 citations

Functional compartments of the yeast Golgi apparatus are defined by the sec7 mutation.

1989 • 249 citations

hsp82 Is an Essential Protein That Is Required in Higher Concentrations for Growth of Cells at Higher Temperatures

1989 • 244 citations

ISOLATION AND CHARACTERIZATION OF MUTATIONS IN THE β-TUBULIN GENE OF SACCHAROMYCES CEREVISIAE

1985 • 234 citations

Nucleotide and deduced amino acid sequences of a GTP-binding protein family with molecular weights of 25,000 from bovine brain.

1988 • 234 citations

Subunit structure of external invertase from Saccharomyces cerevisiae.

1977 • 227 citations

Construction and genetic characterization of temperature-sensitive mutant alleles of the yeast actin gene.

1984 • 177 citations

BET1, BOS1, and SEC22 Are Members of a Group of Interacting Yeast Genes Required for Transport from the Endoplasmic Reticulum to the Golgi Complex

1990 • 176 citations

Invertase signal and mature sequence substitutions that delay intercompartmental transport of active enzyme.

1985 • 167 citations

Sec12p-dependent membrane binding of the small GTP-binding protein Sar1p promotes formation of transport vesicles from the ER.

1991 • 143 citations

A carboxyl-terminal cysteine residue is required for palmitic acid binding and biological activity of the ras-related yeast YPT1 protein.

1988 • 138 citations

Yeast Sec23p acts in the cytoplasm to promote protein transport from the endoplasmic reticulum to the Golgi complex in vivo and in vitro.

1989 • 128 citations

Characterization of new mutants in the early part of the yeast secretory pathway isolated by a [3H]mannose suicide selection.

1987 • 127 citations

Structural and functional dissection of Sec62p, a membrane-bound component of the yeast endoplasmic reticulum protein import machinery.

1990 • 121 citations

Genetic and biochemical evaluation of eucaryotic membrane protein topology: multiple transmembrane domains of Saccharomyces cerevisiae 3-hydroxy-3-methylglutaryl coenzyme A reductase.

1990 • 71 citations

The GTP-binding Sar1 protein is localized to the early compartment of the yeast secretory pathway

1991 • 64 citations

Structural and Functional Dissection of Sec62p, a Membrane-Bound Component of the Yeast Endoplasmic Reticulum Protein Import Machinery

1990 • 51 citations

Protein Glycosylation In Yeast

1987 • 25 citations