Isolation, Characterization, and Partial Purification of a Novel Ubiquitin-Protein Ligase, E3

A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye Binding

1976 • 225,752 citations

A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding

1976 • 200,127 citations

The E6 oncoprotein encoded by human papillomavirus types 16 and 18 promotes the degradation of p53

1990 • 3,896 citations

Cyclin is degraded by the ubiquitin pathway

1991 • 2,355 citations

The HPV-16 E6 and E6-AP complex functions as a ubiquitin-protein ligase in the ubiquitination of p53

1993 • 2,329 citations

The ubiquitin-proteasome proteolytic pathway

1994 • 1,778 citations

THE UBIQUITIN SYSTEM FOR PROTEIN DEGRADATION

1992 • 1,402 citations

Components of ubiquitin-protein ligase system. Resolution, affinity purification, and role in protein breakdown.

1983 • 1,105 citations

The MyoD family and myogenesis: Redundancy, networks, and thresholds

1993 • 1,062 citations

A 20s complex containing CDC27 and CDC16 catalyzes the mitosis-specific conjugation of ubiquitin to cyclin B

1995 • 964 citations

Protein ubiquitination involving an E1–E2–E3 enzyme ubiquitin thioester cascade

1995 • 960 citations

Ubiquitin, proteasomes, and the regulation of intracellular protein degradation

1995 • 862 citations

The cyclosome, a large complex containing cyclin-selective ubiquitin ligase activity, targets cyclins for destruction at the end of mitosis.

1995 • 745 citations

Genes involved in sister chromatid separation are needed for b-type cyclin proteolysis in budding yeast

1995 • 561 citations

THE UBIQUITIN-CONJUGATION SYSTEM

1992 • 561 citations

Cloning and expression of the cDNA for E6-AP, a protein that mediates the interaction of the human papillomavirus E6 oncoprotein with p53.

1993 • 537 citations

Ubiquitin-conjugating enzymes UBC4 and UBC5 mediate selective degradation of short-lived and abnormal proteins.

1990 • 520 citations

The N-end rule

1992 • 464 citations

Multiple ubiquitin-conjugating enzymes participate in the in vivo degradation of the yeast MATα2 repressor

1993 • 397 citations

CDC27Hs colocalizes with CDC16Hs to the centrosome and mitotic spindle and is essential for the metaphase to anaphase transition

1995 • 369 citations

Cyclin Ubiquitination: The destructive end of mitosis

1995 • 304 citations

Functional heterogeneity of ubiquitin carrier proteins.

1985 • 281 citations

Identification of a human ubiquitin-conjugating enzyme that mediates the E6-AP-dependent ubiquitination of p53.

1994 • 259 citations

In vivo degradation of a transcriptional regulator: The yeast α2 repressor

1990 • 243 citations

Ubiquitin-aldehyde: a general inhibitor of ubiquitin-recycling processes.

1987 • 189 citations

Ubiquitin-mediated Processing of NF-κB Transcriptional Activator Precursor p105

1995 • 150 citations

Specificity of binding of NH2-terminal residue of proteins to ubiquitin-protein ligase. Use of amino acid derivatives to characterize specific binding sites.

1988 • 144 citations

Yeast RAD6 encoded ubiquitin conjugating enzyme mediates protein degradation dependent on the N-end-recognizing E3 enzyme.

1991 • 115 citations

Degradation of Proteins with Acetylated Amino Termini by the Ubiquitin System

1989 • 107 citations

Degradation of the tumor suppressor protein p53 by the ubiquitin-mediated proteolytic system requires a novel species of ubiquitin-carrier protein, E2

1994 • 100 citations

N-recognin/Ubc2 interactions in the N-end rule pathway

1993 • 88 citations

Affinity purification of ubiquitin-protein ligase on immobilized protein substrates. Evidence for the existence of separate NH2-terminal binding sites on a single enzyme.

1990 • 73 citations

Binding Sites of Ubiquitin-Protein Ligase

1989 • 70 citations

Purification and characterization of a novel species of ubiquitin-carrier protein, E2, that is involved in degradation of non-“N-end rule” protein substrates

1994 • 66 citations

A ubiquitin-protein ligase specific for type III protein substrates.

1990 • 62 citations

A major ubiquitin conjugation system in wheat germ extracts involves a 15-kDa ubiquitin-conjugating enzyme (E2) homologous to the yeast UBC4/UBC5 gene products.

1993 • 42 citations

Positive control mutations in the MyoD basic region fail to show cooperative DNA binding and transcriptional activation in vitro.

1994 • 41 citations