Studies of the catalytic activities and substrate specificities of Saccharomyces cerevisiae myristoyl-coenzyme A: protein N-myristoyltransferase deletion mutants and human/yeast Nmt chimeras in Escherichia coli and S. cerevisiae.

Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4

1970 • 253,849 citations

A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye Binding

1976 • 225,752 citations

A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding

1976 • 200,127 citations

DNA sequencing with chain-terminating inhibitors

1977 • 69,181 citations

A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae.

1989 • 8,705 citations

“Western Blotting”: Electrophoretic transfer of proteins from sodium dodecyl sulfate-polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A

1981 • 8,657 citations

Rapid and efficient site-specific mutagenesis without phenotypic selection.

1985 • 7,573 citations

Methods in Yeast Genetics

1988 • 4,905 citations

A positive selection for mutants lacking orotidine-5′-phosphate decarboxylase activity in yeast: 5-fluoro-orotic acid resistance

1984 • 2,401 citations

Sequences That Regulate the Divergent GAL1-GAL10 Promoter in Saccharomyces cerevisiae

1984 • 955 citations

THE BIOLOGY AND ENZYMOLOGY OF EUKARYOTIC PROTEIN ACYLATION

1988 • 787 citations

Isothermal titration calorimetry

1990 • 570 citations

A tool for multiple sequence alignment.

1989 • 508 citations

n-Tetradecanoyl is the NH2-terminal blocking group of the catalytic subunit of cyclic AMP-dependent protein kinase from bovine cardiac muscle.

1982 • 395 citations

ADP-ribosylation factor is functionally and physically associated with the Golgi complex.

1990 • 372 citations

Gz, a guanine nucleotide-binding protein with unique biochemical properties.

1990 • 282 citations

The amino terminus of ADP-ribosylation factor (ARF) is a critical determinant of ARF activities and is a potent and specific inhibitor of protein transport.

1992 • 259 citations

ADP ribosylation factor is an essential protein in Saccharomyces cerevisiae and is encoded by two genes.

1990 • 257 citations

Protein N-myristoylation in Escherichia coli: reconstitution of a eukaryotic protein modification in bacteria.

1990 • 245 citations

Regulatory subunit (CNB1 gene product) of yeast Ca2+/calmodulin-dependent phosphoprotein phosphatases is required for adaptation to pheromone.

1992 • 244 citations

Purification and characterization of yeast myristoyl CoA:protein N-myristoyltransferase.

1987 • 241 citations

Amino-terminal processing of proteins by N-myristoylation. Substrate specificity of N-myristoyl transferase.

1987 • 220 citations

ADP-ribosylation factor is required for vesicular trafficking between the endoplasmic reticulum and the cis-Golgi compartment.

1992 • 213 citations

A novel protein kinase homolog essential for protein sorting to the yeast lysosome-like vacuole

1991 • 204 citations

Disruption of the Yeast N -Myristoyl Transferase Gene Causes Recessive Lethality

1989 • 190 citations

Myristoyl CoA:protein N-myristoyltransferase activities from rat liver and yeast possess overlapping yet distinct peptide substrate specificities.

1988 • 187 citations

Evidence for ADP-ribosylation factor (ARF) as a regulator of in vitro endosome-endosome fusion.

1992 • 185 citations

Binding Site of Cerulenin in Fatty Acid Synthetase1

1989 • 170 citations

On the Structure of Fatty Acid Synthetase of Yeast

1980 • 164 citations

Kinetic and structural evidence for a sequential ordered Bi Bi mechanism of catalysis by Saccharomyces cerevisiae myristoyl-CoA:protein N-myristoyltransferase

1991 • 144 citations

Polypeptide chain elongation factor 1 alpha (EF-1 alpha) from yeast: nucleotide sequence of one of the two genes for EF-1 alpha from Saccharomyces cerevisiae.

1984 • 140 citations

Purification and characterization of acyl coenzyme A synthetase from Escherichia coli.

1981 • 129 citations

Protein fatty acid acylation: enzymatic synthesis of an N-myristoylglycyl peptide.

1986 • 123 citations

Mutations of human myristoyl-CoA:protein N-myristoyltransferase cause temperature-sensitive myristic acid auxotrophy in Saccharomyces cerevisiae.

1992 • 115 citations

A genetic and structural analysis of the yeast Vps15 protein kinase: evidence for a direct role of Vps15p in vacuolar protein delivery.

1991 • 107 citations

Lipid modification of proteins: A practical approach

1993 • 102 citations

The substrate specificity of Saccharomyces cerevisiae myristoyl-CoA:protein N-myristoyltransferase. Analysis of myristic acid analogs containing oxygen, sulfur, double bonds, triple bonds, and/or an aromatic residue

1991 • 94 citations

Comparative analysis of the β transducin family with identification of several new members including PWP1, a nonessential gene of Saccharomyces cerevisiae that is divergently transcribed from NMT1

1992 • 88 citations

Isolation of a Saccharomyces cerevisiae long chain fatty acyl:CoA synthetase gene (FAA1) and assessment of its role in protein N-myristoylation.

1992 • 86 citations

N-myristoylation is required for function of the pheromone-responsive G alpha protein of yeast: conditional activation of the pheromone response by a temperature-sensitive N-myristoyl transferase.

1991 • 83 citations

A Saccharomyces cerevisiae Mutant Defective in Saturated Fatty Acid Biosynthesis

1970 • 82 citations

4-oxatetradecanoic acid is fungicidal for Cryptococcus neoformans and inhibits replication of human immunodeficiency virus I.

1992 • 79 citations

Fatty acid biosynthesis in yeast

1978 • 79 citations

Substrate specificity of Saccharomyces cerevisiae myristoyl-CoA: protein N-myristoyltransferase. Analysis of fatty acid analogs containing carbonyl groups, nitrogen heteroatoms, and nitrogen heterocycles in an in vitro enzyme assay and subsequent identification of inhibitors of human immunodeficiency virus I replication.

1992 • 76 citations

Regulation by temperature of the chain length of fatty acids in yeast.

1979 • 72 citations

The Candida albicans myristoyl-CoA:protein N-myristoyltransferase gene. Isolation and expression in Saccharomyces cerevisiae and Escherichia coli.

1992 • 70 citations

Myristic acid auxotrophy caused by mutation of S. cerevisiae myristoyl-CoA:protein N-myristoyltransferase.

1991 • 68 citations

Structural and functional studies of Saccharomyces cerevisiae myristoyl-CoA:protein N-myristoyltransferase produced in Escherichia coli. Evidence for an acyl-enzyme intermediate.

1990 • 68 citations

Analyzing the substrate specificity of Saccharomyces cerevisiae myristoyl-CoA:protein N-myristoyltransferase by co-expressing it with mammalian G protein alpha subunits in Escherichia coli

1991 • 67 citations

S-(2-oxopentadecyl)-CoA, a nonhydrolyzable analog of myristoyl-CoA, is a potent inhibitor of myristoyl-CoA: protein N-myristoyltransferase

1989 • 56 citations

Substitution of cellular fatty acids in yeast cells by the antibiotic cerulenin and exogenous fatty acids

1975 • 52 citations

Altered membrane association of p60v-src and a murine 63-kDa N-myristoyl protein after incorporation of an oxygen-substituted analog of myristic acid.

1989 • 44 citations

Reconstitution of protein N-myristoylation in

1990 • 42 citations

C2,N6-Disubstituted adenosines: synthesis and structure-activity relationships

1989 • 42 citations

Analysis of the compartmentalization of myristoyl-CoA:protein N-myristoyltransferase in Saccharomyces cerevisiae.

1992 • 39 citations

Analogs of palmitoyl-CoA that are substrates for myristoyl-CoA:protein N-myristoyltransferase.

1992 • 38 citations

Functional analysis of protein N-myristoylation: metabolic labeling studies using three oxygen-substituted analogs of myristic acid and cultured mammalian cells provide evidence for protein-sequence-specific incorporation and analog-specific redistribution.

1990 • 35 citations

Possible Involvement of Acetyl Coenzyme A Carboxylase as Well as Fatty Acid Synthetase in the Temperature-Controlled Synthesis of Fatty Acids in Saccharomyces cerevisiae1

1987 • 31 citations

Saturated Fatty Acid Mutant of Saccharomyces cerevisiae with an Intact Fatty Acid Synthetase

1974 • 17 citations