Active site of (A)BC excinuclease. I. Evidence for 5' incision by UvrC through a catalytic site involving Asp399, Asp438, Asp466, and His538 residues.

[19] Rapid and efficient site-specific mutagenesis without phenotypic selection

1987 • 5,413 citations

Discontinuities in the DNA synthesized in an Excision-defective strain of Escherichia coli following ultraviolet irradiation

1968 • 1,089 citations

Structural basis for the 3′-5′ exonuclease activity of Escherichia coli DNA polymerase I: a two metal ion mechanism.

1991 • 1,059 citations

DNA REPAIR ENZYMES

1988 • 822 citations

A novel repair enzyme: UVRABC excision nuclease of Escherichia coli cuts a DNA strand on both sides of the damaged region

1983 • 618 citations

Structure of DNase I at 2.0 Å resolution suggests a mechanism for binding to and cutting DNA

1986 • 327 citations

Structure refined to 2Å of a nicked DNA octanucleotide complex with DNase I

1988 • 293 citations

The 3′-5′ exonuclease of DNA polymerase I of Escherichia coli: contribution of each amino acid at the active site to the reaction.

1991 • 279 citations

Crystal structure of Penicillium citrinum P1 nuclease at 2.8 A resolution.

1991 • 256 citations

Studies on radiation-sensitive mutants of E. coli

1968 • 218 citations

The (A)BC excinuclease of Escherichia coli has only the UvrB and UvrC subunits in the incision complex.

1989 • 204 citations

How does RNase H recognize a DNA.RNA hybrid?

1991 • 188 citations

A new mechanism for repairing oxidative damage to DNA: (A)BC excinuclease removes AP sites and thymine glycols from DNA

1989 • 177 citations

Amplification and purification of UvrA, UvrB, and UvrC proteins of Escherichia coli.

1985 • 124 citations

Purification and properties of the uvrA protein from Escherichia coli.

1982 • 122 citations

Formation and enzymatic properties of the UvrB.DNA complex.

1990 • 119 citations

DNase I footprint of ABC excinuclease.

1987 • 106 citations

Human lipoprotein lipase. Analysis of the catalytic triad by site-directed mutagenesis of Ser-132, Asp-156, and His-241.

1992 • 104 citations

Active site of (A)BC excinuclease. II. Binding, bending, and catalysis mutants of UvrB reveal a direct role in 3‘ and an indirect role in 5‘ incision.

1992 • 102 citations

Two histidine residues are essential for ribonuclease T1 activity as is the case for ribonuclease A

1987 • 101 citations

Sequences of theE. coli uvrCgene and protein

1984 • 100 citations

Site-directed mutagenesis studies with EcoRV restriction endonuclease to identify regions involved in recognition and catalysis

1991 • 81 citations

ATP-dependent partitioning of the DNA template into supercoiled domains by Escherichia coli UvrAB.

1991 • 81 citations

Isolation of BamHI variants with reduced cleavage activities

1991 • 72 citations

Identification of the different intermediates in the interaction of (A)BC excinuclease with its substrates by DNase I footprinting on two uniquely modified oligonucleotides

1991 • 66 citations

Interactions of the acid and base catalysts on staphylococcal nuclease as studied in a double mutant

1991 • 64 citations

Sequences of theE. coli uvrBgene and protein

1986 • 63 citations

Chromosomal Location of the Bacillus subtilis Aspartokinase II Gene and Nucleotide Sequence of the Adjacent Genes Homologous to uvrC and trx of Escherichia coli

1989 • 54 citations

ABC excinuclease incises both 5' and 3' to the CC-1065-DNA adduct and its incision activity is stimulated by DNA helicase II and DNA polymerase I

1988 • 45 citations

Impaired incision of ultraviolet-irradiated deoxyribonucleic acid in uvrC mutants of Escherichia coli

1980 • 25 citations

Structure and catalytic function of nucleases

1991 • 25 citations

Excision of pyrimidine dimers in toluene-treated Escherichia coli

1976 • 22 citations

The C-terminal half of UvrC protein is sufficient to reconstitute (A)BC excinuclease.

1991 • 20 citations

Structure of DNase I

1991 • 18 citations

Repair of helix-stabilizing anthramycin-N2 guanine DNA adducts by UVRA and UVRB proteins

1991 • 16 citations

Single-strand breakage of DNA in UV-irradiated uvrA, uvrB, and uvrC mutants of Escherichia coli

1985 • 13 citations

Deleted Work

1955 • 0 citations