Expression of a dominant-negative mutant human insulin receptor in the muscle of transgenic mice.

Single-Step Method of RNA Isolation by Acid Guanidinium Thiocyanate–Phenol–Chloroform Extraction

1987 • 64,270 citations

Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction

1987 • 45,673 citations

Manipulating the mouse embryo: A laboratory manual

1986 • 4,548 citations

Current protocols in molecular biology

1988 • 4,543 citations

Manipulating the mouse embryo: A laboratory manual

1986 • 2,165 citations

The Effect of Insulin on the Disposal of Intravenous Glucose: Results from Indirect Calorimetry and Hepatic and Femoral Venous Catheterization

1981 • 1,728 citations

Structure of the insulin receptor substrate IRS-1 defines a unique signal transduction protein

1991 • 1,590 citations

The human insulin receptor cDNA: The structural basis for hormone-activated transmembrane signalling

1985 • 1,485 citations

Insulin Stimulates the Phosphorylation of the 95,000-Dalton Subunit of Its Own Receptor

1982 • 1,138 citations

HINDLIMB MUSCLE FIBER POPULATIONS OF FIVE MAMMALS

1973 • 1,133 citations

Replacement of insulin receptor tyrosine residues 1162 and 1163 compromises insulin-stimulated kinase activity and uptake of 2-deoxyglucose

1986 • 1,100 citations

Introns increase transcriptional efficiency in transgenic mice.

1988 • 733 citations

Human insulin receptors mutated at the ATP-binding site lack protein tyrosine kinase activity and fail to mediate postreceptor effects of insulin.

1987 • 648 citations

Phosphorylation activates the insulin receptor tyrosine protein kinase.

1983 • 558 citations

Adipose cell hyperplasia and enhanced glucose disposal in transgenic mice overexpressing GLUT4 selectively in adipose tissue.

1993 • 510 citations

Heterologous introns can enhance expression of transgenes in mice.

1991 • 489 citations

A cascade of tyrosine autophosphorylation in the beta-subunit activates the phosphotransferase of the insulin receptor.

1988 • 467 citations

A rapid method for the determination of glycogen content and radioactivity in small quantities of tissue or isolated hepatocytes

1976 • 417 citations

A mutant insulin receptor with defective tyrosine kinase displays no biologic activity and does not undergo endocytosis.

1987 • 403 citations

Replacement of lysine residue 1030 in the putative ATP-binding region of the insulin receptor abolishes insulin- and antibody-stimulated glucose uptake and receptor kinase activity.

1987 • 399 citations

Transgenic Mice as Probes into Complex Systems

1989 • 368 citations

Structure of the human insulin receptor gene and characterization of its promoter.

1989 • 349 citations

Insulin receptor kinase in human skeletal muscle from obese subjects with and without noninsulin dependent diabetes.

1987 • 344 citations

An in vitro human muscle preparation suitable for metabolic studies. Decreased insulin stimulation of glucose transport in muscle from morbidly obese and diabetic subjects.

1988 • 292 citations

Decreased kinase activity of insulin receptors from adipocytes of non-insulin-dependent diabetic subjects.

1987 • 228 citations

Muscle Creatine Kinase Sequence Elements Regulating Skeletal and Cardiac Muscle Expression in Transgenic Mice

1989 • 204 citations

Human Diabetes Associated with a Mutation in the Tyrosine Kinase Domain of the Insulin Receptor

1989 • 192 citations

Role of glucose transporters in the cellular insulin resistance of type II non-insulin-dependent diabetes mellitus.

1988 • 169 citations

Effects of insulin incubation on insulin binding, glucose transport, and insulin degradation by isolated rat adipocytes. Evidence for hormone-induced desensitization at the receptor and postreceptor level.

1980 • 168 citations

Detection of an Alteration in the Insulin-Receptor Gene in a Patient with Insulin Resistance, Acanthosis Nigricans, and the Polycystic Ovary Syndrome (Type A Insulin Resistance)

1988 • 153 citations

High-level expression of human insulin receptor cDNA in mouse NIH 3T3 cells.

1987 • 151 citations

Defect in insulin receptor phosphorylation in erythrocytes and fibroblasts associated with severe insulin resistance.

1984 • 146 citations

Alternatively spliced variants of the insulin receptor protein. Expression in normal and diabetic human tissues.

1992 • 121 citations

A naturally occurring mutation of insulin receptor alanine 1134 impairs tyrosine kinase function and is associated with dominantly inherited insulin resistance.

1990 • 118 citations

Defect in skeletal muscle phosphatidylinositol-3-kinase in obese insulin-resistant mice.

1993 • 115 citations

Myosin light chain enhancer activates muscle-specific, developmentally regulated gene expression in transgenic mice.

1989 • 113 citations

Prevalence of Mutations in the Insulin Receptor Gene in Subjects With Features of the Type A Syndrome of Insulin Resistance

1994 • 111 citations

The insulin receptor with phenylalanine replacing tyrosine-1146 provides evidence for separate signals regulating cellular metabolism and growth.

1990 • 107 citations

Transdominant inhibition of tyrosine kinase activity in mutant insulin/insulin-like growth factor I hybrid receptors.

1991 • 103 citations

Detection of Mutations in Insulin-Receptor Gene in NIDDM Patients by Analysis of Single-Stranded Conformation Polymorphisms

1991 • 95 citations

Replacement of insulin receptor tyrosine residues 1162 and 1163 does not alter the mitogenic effect of the hormone.

1988 • 92 citations

Insulin-Receptor Kinase Activity of Adipose Tissue From Morbidly Obese Humans With and Without NIDDM

1987 • 86 citations

Insulin receptors with defective tyrosine kinase inhibit normal receptor function at the level of substrate phosphorylation.

1988 • 82 citations

The role of insulin receptor autophosphorylation in signal transduction.

1991 • 80 citations

A Mutation in the Tyrosine Kinase Domain of the Insulin Receptor Associated with Insulin Resistance in an Obese Woman*

1991 • 74 citations

Biologic activities of naturally occurring human insulin receptor mutations. Evidence that metabolic effects of insulin can be mediated by a kinase-deficient insulin receptor mutant

1991 • 67 citations

Detection of mutations in the insulin receptor gene in patients with insulin resistance by analysis of single-stranded conformational polymorphisms

1992 • 62 citations

The pathway mediating insulin's effects on pyruvate dehydrogenase bypasses the insulin receptor tyrosine kinase

1991 • 58 citations

A mutation (Trp1193?Leu1193) in the tyrosine kinase domain of the insulin receptor associated with type A syndrome of insulin resistance

1993 • 49 citations

Functional Properties of a Naturally Occurring Trp¹²⁰⁰→ Ser¹²⁰⁰Mutation of the Insulin Receptor

1990 • 48 citations

Substitution of glutamic acid for alanine 1135 in the putative “catalytic loop” of the tyrosine kinase domain of the human insulin receptor. A mutation that impairs proteolytic processing into subunits and inhibits receptor tyrosine kinase activity.

1993 • 40 citations

Phosphorylation state and biological function of a mutant human insulin receptor Val996.

1990 • 36 citations

In vivo stimulation of the insulin receptor kinase in human skeletal muscle. Correlation with insulin-stimulated glucose disposal during euglycemic clamp studies.

1991 • 35 citations

A Microtiter Well Assay System to Measure Insulin Activation of Insulin Receptor Kinase in Intact Human Mononuclear Cells: Decreased Insulin Effect in Cells From Patients With NIDDM

1993 • 27 citations

Ala1048→Asp Mutation in the Kinase Domain of Insulin Receptor Causes Defective Kinase Activity and Insulin Resistance

1993 • 24 citations

Transmembrane signalling by insulin via an insulin receptor mutated at tyrosines 1158, 1162, and 1163

1991 • 23 citations

Muscle-Specific Expression of Human Insulin Receptor in Transgenic Mice

1993 • 16 citations

A glycine-1008 to valine mutation in the insulin receptor in a woman with type A insulin resistance.

1993 • 9 citations

Loss of insulin binding and insulin receptor mRNA in a transformed human fetal fibroblast cell line

1990 • 4 citations