The effect of N-linked glycosylation on activity of the Na(+)- and Cl(-)-dependent serotonin transporter expressed using recombinant baculovirus in insect cells

Molecular cloning: A laboratory manual

1990 • 85,659 citations

Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.

1979 • 54,907 citations

LIGAND: A versatile computerized approach for characterization of ligand-binding systems

1980 • 8,534 citations

A rapid, sensitive, and specific method for the determination of protein in dilute solution

1973 • 2,518 citations

Isolation of monoclonal antibodies specific for human c-myc proto-oncogene product.

1985 • 2,456 citations

Structural features in eukaryotic mRNAs that modulate the initiation of translation.

1991 • 1,849 citations

A Manual of Methods for Baculovirus Vectors and Insect Cell Culture Procedures.

1987 • 1,703 citations

An hsp70-like protein in the ER: Identity with the 78 kd glucose-regulated protein and immunoglobulin heavy chain binding protein

1986 • 1,457 citations

Analysis of radioligand binding experiments

1985 • 1,247 citations

A Short Polypeptide Marker Sequence Useful for Recombinant Protein Identification and Purification

1988 • 934 citations

Antidepressant- and cocaine-sensitive human serotonin transporter: molecular cloning, expression, and chromosomal localization.

1993 • 883 citations

Cloning and expression of a functional serotonin transporter from rat brain

1991 • 822 citations

Projection structure of rhodopsin

1993 • 758 citations

Cloning of a Serotonin Transporter Affected by Antidepressants

1991 • 613 citations

The molecular mechanism of "ecstasy" [3,4-methylenedioxy-methamphetamine (MDMA)]: serotonin transporters are targets for MDMA-induced serotonin release.

1992 • 526 citations

Mechanism of Transport and Storage of Neurotransmitter

1987 • 471 citations

From synapse to vesicle: The reuptake and storage of biogenic amine neurotransmitters

1993 • 461 citations

Dopamine transporter site-directed mutations differentially alter substrate transport and cocaine binding.

1992 • 393 citations

Stable expression of biogenic amine transporters reveals differences in inhibitor sensitivity, kinetics, and ion dependence.

1994 • 375 citations

Two-dimensional crystallization of membrane proteins

1992 • 292 citations

2D crystallization: from art to science

1992 • 281 citations

Three-dimensional cryo-electron microscopy of the calcium ion pump in the sarcoplasmic reticulum membrane

1993 • 231 citations

Active transport of 5-hydroxytryptamine by plasma membrane vesicles isolated from human blood platelets.

1977 • 224 citations

Mutational analysis of beta-adrenergic receptor glycosylation.

1990 • 213 citations

The role of the asparagine-linked oligosaccharides of the alpha subunit in the secretion and assembly of human chorionic gonadotrophin.

1988 • 203 citations

Neurotransmitter transporters (plus): a promising new gene family

1992 • 162 citations

Evidence for an Imipramine-sensitive Serotonin Transporter in Human Placental Brush-border Membranes

1989 • 158 citations

Expression of a cloned .gamma.-aminobutyric acid transporter in mammalian cells

1992 • 144 citations

Human norepinephrine transporter. Biosynthetic studies using a site-directed polyclonal antibody.

1994 • 119 citations

Regulation of the human serotonin transporter. Cholera toxin-induced stimulation of serotonin uptake in human placental choriocarcinoma cells is accompanied by increased serotonin transporter mRNA levels and serotonin transporter-specific ligand binding.

1993 • 117 citations

Only one of the charged amino acids located in the transmembrane alpha-helices of the gamma-aminobutyric acid transporter (subtype A) is essential for its activity.

1993 • 107 citations

Overproduction of membrane proteins

1992 • 104 citations

Solubilization of the platelet plasma membrane serotonin transporter in an active form.

1980 • 97 citations

Binding of some antidepressants to the 5-hydroxytryptamine transporter in brain and platelets

1990 • 96 citations

Identification of tryptophan residues critical for the function and targeting of the gamma-aminobutyric acid transporter (subtype A).

1994 • 94 citations

Projection Structure of Halorhodopsin from Halobacterium halobium at 6 Å Resolution Obtained by Electron Cryo-microscopy

1993 • 93 citations

Expression of the functional mature chloroplast triose phosphate translocator in yeast internal membranes and purification of the histidine-tagged protein by a single metal-affinity chromatography step.

1993 • 91 citations

Properties and purification of an active biotinylated lactose permease from Escherichia coli.

1993 • 89 citations

Functional expression of Shaker K+ channels in a baculovirus-infected insect cell line

1990 • 89 citations

Amino acid neurotransmitter transporters: Structure, function, and molecular diversity

1993 • 74 citations

One-step purification of the serotonin transporter located at the human platelet plasma membrane.

1992 • 68 citations

Binding of the cocaine analog 2 beta-carbomethoxy-3 beta-(4-[125I]iodophenyl)tropane to serotonin and dopamine transporters: different ionic requirements for substrate and 2 beta-carbomethoxy-3 beta-(4-[125I]iodophenyl)tropane binding.

1993 • 66 citations

The glucose transporter of Escherichia coli

1993 • 61 citations

The Baculovirus-Infected Insect Cell as a Eukaryotic Gene Expression System

1992 • 51 citations

Identification of domains of a cloned rat brain GABA transporter which are not required for its functional expression

1993 • 48 citations

Structural and functional studies on the sodium- and chloride-coupled .gamma.-aminobutyric acid transporter: deglycosylation and limited proteolysis

1989 • 46 citations

Partial purification and characterization of the human placental serotonin transporter

1993 • 44 citations

Partial purification of the 5-hydroxytryptophan-reuptake system from human blood platelets using a citalopram-derived affinity resin

1990 • 32 citations

Partial purification and characterization of the sodium-ion-coupled 5-hydroxytryptamine transporter of rat cerebral cortex

1992 • 28 citations

Reconstitution of the human placental 5-hydroxytryptamine transporter in a catalytically active form after detergent solubilization

1992 • 28 citations

Role of essential sulfhydryl groups in drug interactions at the neuronal 5-HT transporter. Differences between amphetamines and 5-HT uptake inhibitors.

1992 • 24 citations

Solubilization and partial purification of the high affinity [³H] imipramine binding site from human platelets

1982 • 19 citations

Non‐lysosomal degradation of misfolded human lysozymes with and without an asparagine‐linked glycosylation site

1992 • 14 citations