DNA Immunization with Minigenes: Low Frequency of Memory Cytotoxic T Lymphocytes and Inefficient Antiviral Protection Are Rectified by Ubiquitination

Development of Virus-Like Particle Technology from Small Highly Symmetric to Large Complex Virus-Like Particle Structures

2013 • 5,819 citations

Heterologous Protection Against Influenza by Injection of DNA Encoding a Viral Protein

1993 • 2,330 citations

Cytotoxicity mediated by T cells and natural killer cells is greatly impaired in perforin-deficient mice

1994 • 1,772 citations

Genetic immunization is a simple method for eliciting an immune response

1992 • 1,505 citations

Possible role of flanking nucleotides in recognition of the AUG initiator codon by eukaryotic ribosomes

1981 • 1,265 citations

DNA vaccines: protective immunizations by parenteral, mucosal, and gene-gun inoculations.

1993 • 1,122 citations

Rapid Effector Function in CD8+ Memory T Cells

1997 • 652 citations

Gene vaccination with naked plasmid DNA: mechanism of CTL priming.

1996 • 494 citations

CD8+ T cells clear influenza virus by perforin or Fas-dependent processes.

1997 • 493 citations

Recognition of AUG and alternative initiator codons is augmented by G in position +4 but is not generally affected by the nucleotides in positions +5 and +6

1997 • 486 citations

Immune function in mice lacking the perforin gene.

1994 • 474 citations

Lymphocytic Choriomeningitis Virus

2016 • 423 citations

Patterns of cytokine gene expression by CD4+ T cells from young and old mice.

1993 • 357 citations

Induction of cytotoxic T lymphocytes by intramuscular immunization with plasmid DNA is facilitated by bone marrow-derived cells.

1996 • 353 citations

A pentapeptide as minimal antigenic determinant for MHC class I-restricted T lymphocytes

1989 • 339 citations

Enhanced CTL responses mediated by plasmid DNA immunogens encoding costimulatory molecules and cytokines.

1997 • 307 citations

Efficient processing of an antigenic sequence for presentation by MHC class I molecules depends on its neighboring residues in the protein

1991 • 303 citations

Effect of intron A from human cytomegalovirus (Towne) immediate-early gene on heterologous expression in mammalian cells

1991 • 297 citations

Protection against mycoplasma infection using expression-library immunization

1995 • 296 citations

Priming of Cytotoxic T Lymphocytes by DNA Vaccines: Requirement for Professional Antigen Presenting Cells and Evidence for Antigen Transfer from Myocytes

1997 • 296 citations

Differential dependence on target site tissue for gene gun and intramuscular DNA immunizations.

1997 • 287 citations

CD4-deficient mice have reduced levels of memory cytotoxic T lymphocytes after immunization and show diminished resistance to subsequent virus challenge

1996 • 287 citations

The roles of perforin‐ and Fas‐dependent cytotoxicity in protection against cytopathic and noncytopathic viruses

1995 • 286 citations

DNA immunization: ubiquitination of a viral protein enhances cytotoxic T-lymphocyte induction and antiviral protection but abrogates antibody induction

1997 • 269 citations

To kill or to cure: options in host defense against viral infection

1996 • 268 citations

Ubiquitin as a degradation signal.

1992 • 261 citations

The dominant role of bone marrow-derived cells in CTL induction following plasmid DNA immunization at different sites.

1997 • 257 citations

Enhancement of cellular and humoral immune responses to hepatitis C virus core protein using DNA-based vaccines augmented with cytokine-expressing plasmids.

1997 • 257 citations

Improvement of hepatitis B virus DNA vaccines by plasmids coexpressing hepatitis B surface antigen and interleukin-2

1997 • 237 citations

Antigen processing in vivo and the elicitation of primary CTL responses.

1995 • 226 citations

A case for cytokines as effector molecules in the resolution of virus infection

1993 • 216 citations

Induction of cytotoxic T lymphocytes and antitumor immunity with DNA vaccines expressing single T cell epitopes.

1996 • 201 citations

Examination of Parameters Affecting the Elicitation of Humoral Immune Responses by Particle Bombardment-Mediated Genetic Immunization

1993 • 188 citations

DNA immunization confers protection against lethal lymphocytic choriomeningitis virus infection

1995 • 185 citations

A "string-of-beads" vaccine, comprising linked minigenes, confers protection from lethal-dose virus challenge

1993 • 176 citations

Fine dissection of a nine amino acid glycoprotein epitope, a major determinant recognized by lymphocytic choriomeningitis virus-specific class I-restricted H-2Db cytotoxic T lymphocytes.

1988 • 166 citations

A protein particle vaccine containing multiple malaria epitopes

1997 • 150 citations

DNA vaccination against persistent viral infection

1995 • 147 citations

Minimal epitopes expressed in a recombinant polyepitope protein are processed and presented to CD8+ cytotoxic T cells: implications for vaccine design.

1995 • 142 citations

DNA vaccines

1996 • 140 citations

Molecular analyses of a five-amino-acid cytotoxic T-lymphocyte (CTL) epitope: an immunodominant region which induces nonreciprocal CTL cross-reactivity

1989 • 138 citations

Successful nucleic acid based immunization of newborn chimpanzees against hepatitis B virus

1997 • 137 citations

The variability in activity of the universally expressed human cytomegalovirus immediate early gene 1 enhancer/promoter in transgenic mice

1991 • 136 citations

Enhancement of immune response to naked DNA vaccine by immunization with transfected dendritic cells

1997 • 135 citations

Protection against lethal cytomegalovirus infection by a recombinant vaccine containing a single nonameric T-cell epitope

1991 • 135 citations

Vaccination and protection from a lethal viral infection: Identification, incorporation, and use of a cytotoxic T lymphocyte glycoprotein epitope

1990 • 132 citations

Use of DNA Encoding Influenza Hemagglutinin as an Avian Influenza Vaccine

1993 • 125 citations

Neonatal DNA immunization with a plasmid encoding an internal viral protein is effective in the presence of maternal antibodies and protects against subsequent viral challenge

1997 • 116 citations

MHC class I-associated peptides produced from endogenous gene products with vastly different efficiencies.

1997 • 110 citations

Protective CTL-dependent immunity and enhanced immunopathology in mice immunized by particle bombardment with DNA encoding an internal virion protein.

1995 • 109 citations

DNA immunization of neonates induces immunity despite the presence of maternal antibody.

1997 • 99 citations

The antiviral activity of immune CD8+ T cells is dependent on interferon-gamma.

1991 • 98 citations

Cytolytically active memory CTL present in lymphocytic choriomeningitis virus-immune mice after clearance of virus infection.

1997 • 97 citations

Increasing gene expression in yeast by fusion to ubiquitin

1989 • 95 citations

DNA vaccines: A novel approach to immunization

1995 • 92 citations

A multivalent minigene vaccine, containing B-cell, cytotoxic T-lymphocyte, and Th epitopes from several microbes, induces appropriate responses in vivo and confers protection against more than one pathogen

1997 • 90 citations

Immune Response to Neonatal Genetic Immunization

1997 • 90 citations

DNA immunization

1996 • 89 citations

Costimulation provided by DNA immunization enhances antitumor immunity.

1997 • 79 citations

Class I MHC can present an endogenous peptide to cytotoxic T lymphocytes.

1989 • 62 citations

DNA Immunization of Newborn Mice with a Plasmid-Expressing Nucleoprotein of Influenza Virus

1996 • 62 citations

Uncovering subdominant cytotoxic T-lymphocyte responses in lymphocytic choriomeningitis virus-infected BALB/c mice

1997 • 58 citations

Low-Affinity Cytotoxic T-Lymphocytes Require IFN-γ to Clear an Acute Viral Infection

1997 • 45 citations

DNA immunization: Effects of vehicle and route of administration on the induction of protective antiviral immunity

1996 • 42 citations

Differential effects of flanking residues on presentation of epitopes from chimeric peptides

1994 • 40 citations

A recombinant minigene vaccine containing a nonameric cytotoxic-T-lymphocyte epitope confers limited protection against Listeria monocytogenes infection

1996 • 29 citations

Animal Virus Pathogenesis: A Practical Approach

1990 • 20 citations

Polymorphism of cytotoxic T-lymphocyte clones that recognize a defined nine-amino-acid immunodominant domain of lymphocytic choriomeningitis virus glycoprotein

1989 • 15 citations

Antiviral protective immunity induced by major histocompatibility complex class I molecule-restricted viral T-lymphocyte epitopes inserted in various positions in immunologically self and nonself proteins

1995 • 13 citations

Presentation by a major histocompatibility complex class I molecule of nucleoprotein peptide expressed in two different genes of an influenza virus transfectant.

1995 • 12 citations

DNA Immunization in an Arenavirus Model

1996 • 4 citations