Distinct binding sites of Ala48-hirudin1-47 and Ala48-hirudin48-65 on alpha-thrombin.

Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4

1970 • 253,849 citations

DNA sequencing with chain-terminating inhibitors

1977 • 69,181 citations

[32] Oligonucleotide-directed mutagenesis of DNA fragments cloned into M13 vectors

1983 • 1,093 citations

Structure of crystalline α-chymotrypsin

1972 • 675 citations

[36] High-sensitivity sequencing with a gas-phase sequenator

1983 • 670 citations

Kinetics of the inhibition of thrombin by hirudin

1986 • 581 citations

The slow-binding and slow, tight-binding inhibition of enzyme-catalysed reactions

1982 • 488 citations

Determination of the operational molarity of solutions of bovine α-chymotrypsin, trypsin, thrombin and factor Xa by spectrofluorimetric titration

1973 • 376 citations

A new siliconized‐glass fiber as support for protein‐chemical analysis of electroblotted proteins.

1988 • 319 citations

[69] Hirudin as an inhibitor of thrombin

1970 • 311 citations

Anticoagulant Activity of Synthetic Hirudin Peptides

1989 • 203 citations

THROMBIN SPECIFICITY*

1981 • 202 citations

[13] Prothrombin

1976 • 197 citations

Thrombin^a

1986 • 186 citations

Use of site-directed mutagenesis to investigate the basis for the specificity of hirudin

1988 • 170 citations

Anion-binding exosite of human .alpha.-thrombin and fibrin(ogen) recognition

1988 • 161 citations

Solution structure of recombinant hirudin and the Lys-47 .fwdarw. Glu mutants: a nuclear magnetic resonance and hybrid distance geometry-dynamical simulated annealing study

1989 • 155 citations

The functional domain of hirudin, a thrombin‐specific inhibitor

1983 • 151 citations

Protides of the Biological Fluids

1975 • 130 citations

Conformation of recombinant desulfatohirudin in aqueous solution determined by nuclear magnetic resonance

1989 • 126 citations

Interaction of hirudin with thrombin: identification of a minimal binding domain of hirudin that inhibits clotting activity

1988 • 124 citations

Identification of regions of .alpha.-thrombin involved in its interaction with hirudin

1987 • 122 citations

[18] Oligonucleotide-directed construction of mutations via gapped duplex DNA

1987 • 119 citations

Antithrombin properties of C‐terminus of hirudin using synthetic unsulfated N^α‐acetyl‐hirudin_45–65

1987 • 108 citations

Activation of prothrombin with (taipan snake) venom

1973 • 107 citations

The Complete Covalent Structure of Hirudin. Localization of the Disulfide Bonds

1985 • 104 citations

The conformations of hirudin in solution: a study using nuclear magnetic resonance, distance geometry and restrained molecular dynamics

1987 • 99 citations

Interaction of site specific hirudin variants with α-thrombin

1988 • 89 citations

[54] Hirudin

1976 • 87 citations

Thrombin Active-Site Regions

1986 • 85 citations

Expression, secretion and processing of hirudin in E. coli using the alkaline phosphatase signal sequence

1986 • 78 citations

Proflavine binding within the fibrinopeptide groove adjacent to the catalytic site of human .alpha.-thrombin

1984 • 74 citations

Physical evidence for an apolar binding site near the catalytic center of human α-thrombin

1977 • 73 citations

The use of sequence-specific antibodies to identify a secondary binding site in thrombin.

1988 • 71 citations

Isolation and Characterization of Hirudin Isoinhibitors and Sequence Analysis of Hirudin PA

1986 • 71 citations

Staphylocoagulase-Binding Region in Human Prothrombin12

1985 • 52 citations

Analysis of the secondary structure of hirudin and the mechanism of its interaction with thrombin

1988 • 52 citations

Identification of the primary structural defect in the dysthrombin thrombin Quick I: substitution of cysteine for arginine-382

1988 • 48 citations

Point mutations modifying the thrombin inhibition kinetics and antithrombotic activity in vivo of recombinant hirudin

1989 • 39 citations

Thrombin Anion-binding Exosite Interactions with Heparin and Various Polyanions

1989 • 36 citations

Thrombin specificity with tripeptide chromogenic substrates: comparison of human and bovine thrombins with and without fibrinogen clotting activities.

1986 • 34 citations

Influence of Chain Shortening on the Inhibitor Properties of Hirudin and Eglinc

1987 • 22 citations

Thrombin clotting activity measurement and standardization with lyophilized plasma.

1986 • 8 citations

Thrombin clotting activity measurement and standardization with lyophilized plasma.

1986 • 7 citations