Cloning and characterization of a Saccharomyces cerevisiae gene encoding a new member of the ubiquitin-conjugating protein family

Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4

1970 • 253,849 citations

Improved tools for biological sequence comparison.

1988 • 11,455 citations

Transformation of intact yeast cells treated with alkali cations

1983 • 7,688 citations

Current protocols in molecular biology

1988 • 7,584 citations

Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes

1986 • 6,284 citations

Methods in Yeast Genetics

1988 • 4,905 citations

A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity

1984 • 4,899 citations

[12] One-step gene disruption in yeast

1983 • 3,302 citations

DNA sequence analysis with a modified bacteriophage T7 DNA polymerase.

1987 • 2,266 citations

Two differentially regulated mRNAs with different 5′ ends encode secreted and intracellular forms of yeast invertase

1982 • 1,539 citations

A Multiubiquitin Chain Is Confined to Specific Lysine in a Targeted Short-Lived Protein

1989 • 1,463 citations

Components of ubiquitin-protein ligase system. Resolution, affinity purification, and role in protein breakdown.

1983 • 1,105 citations

The yeast DNA repair gene RAD6 encodes a ubiquitin-conjugating enzyme

1987 • 731 citations

ATP-dependent conjugation of reticulocyte proteins with the polypeptide required for protein degradation.

1980 • 530 citations

Ubiquitin-conjugating enzymes UBC4 and UBC5 mediate selective degradation of short-lived and abnormal proteins.

1990 • 520 citations

Universality and Structure of the N-end Rule

1989 • 472 citations

Ubiquitin-mediated protein degradation.

1988 • 453 citations

Use of a screen for synthetic lethal and multicopy suppressee mutants to identify two new genes involved in morphogenesis in Saccharomyces cerevisiae.

1991 • 442 citations

The Yeast Cell Cycle Gene CDC34 Encodes a Ubiquitin-Conjugating Enzyme

1988 • 435 citations

Duplication of Spindle Plaques and Integration of the Yeast Cell Cycle

1974 • 419 citations

Ubiquitin-activating enzyme. Mechanism and role in protein-ubiquitin conjugation.

1982 • 413 citations

Ubiquitin-Mediated Pathways for Intracellular Proteolysis

1987 • 409 citations

The recognition component of the N-end rule pathway.

1990 • 388 citations

"Covalent affinity" purification of ubiquitin-activating enzyme.

1982 • 310 citations

The isolation, genetics and survival characteristics of ultraviolet light-sensitive mutants in yeast

1968 • 286 citations

Functional heterogeneity of ubiquitin carrier proteins.

1985 • 281 citations

In vivo degradation of a transcriptional regulator: The yeast α2 repressor

1990 • 243 citations

A 25-kilodalton ubiquitin carrier protein (E2) catalyzes multi-ubiquitin chain synthesis via lysine 48 of ubiquitin.

1990 • 212 citations

The RAD6 protein of Saccharomyces cerevisiae polyubiquitinates histones, and its acidic domain mediates this activity.

1988 • 196 citations

Ubiquitin-conjugating enzymes: novel regulators of eukaryotic cells

1990 • 184 citations

UBC1 encodes a novel member of an essential subfamily of yeast ubiquitin-conjugating enzymes involved in protein degradation.

1990 • 160 citations

Mutation of cysteine-88 in the Saccharomyces cerevisiae RAD6 protein abolishes its ubiquitin-conjugating activity and its various biological functions.

1990 • 155 citations

LACK OF CHEMICALLY INDUCED MUTATION IN REPAIR-DEFICIENT MUTANTS OF YEAST

1974 • 147 citations

Specificity of binding of NH2-terminal residue of proteins to ubiquitin-protein ligase. Use of amino acid derivatives to characterize specific binding sites.

1988 • 144 citations

A uniform isopeptide-linked multiubiquitin chain is sufficient to target substrate for degradation in ubiquitin-mediated proteolysis.

1990 • 136 citations

The resolution and characterization of putative ubiquitin carrier protein isozymes from rabbit reticulocytes.

1988 • 133 citations

Codon usage tabulated from the GenBank genetic sequence data

1990 • 125 citations

Functional diversity among putative E2 isozymes in the mechanism of ubiquitin-histone ligation.

1988 • 109 citations

The rhp6+ gene of Schizosaccharomyces pombe: a structural and functional homolog of the RAD6 gene from the distantly related yeast Saccharomyces cerevisiae.

1990 • 88 citations

Recombination and mutagenesis in rad6 mutants of Saccharomyces cerevisiae: Evidence for multiple functions of the RAD6 gene

1981 • 80 citations

Affinity purification of ubiquitin-protein ligase on immobilized protein substrates. Evidence for the existence of separate NH2-terminal binding sites on a single enzyme.

1990 • 73 citations

A ubiquitin-protein ligase specific for type III protein substrates.

1990 • 62 citations

DNA sequence analysis on the IBM-PC

1984 • 61 citations

The human ubiquitin carrier protein E2(Mr = 17,000) is homologous to the yeast DNA repair gene RAD6.

1990 • 54 citations

Ubiquitin-dependent proteolytic pathway in wheat germ: isolation of multiple forms of ubiquitin-activating enzyme, E1

1989 • 52 citations

Ubiquitin carrier protein-catalyzed ubiquitin transfer to histones. Mechanism and specificity.

1988 • 49 citations

Identifying Mutations in Duplicated Functions in Saccharomyces cerevisiae: Recessive Mutations in HMG-CoA Reductase Genes

1987 • 49 citations

A ubiquitin carrier protein from wheat germ is structurally and functionally similar to the yeast DNA repair enzyme encoded by RAD6.

1989 • 47 citations

A conserved sequence in histone H2A which is a ubiquitination site in higher eucaryotes is not required for growth in Saccharomyces cerevisiae.

1990 • 43 citations

Purification, Characterization, and Rapid Inactivation of Thermolabile Ubiquitin-activating Enzyme from the Mammalian Cell Cycle Mutant ts85

1989 • 28 citations

Degradation of proteins by the ubiquitin-mediated proteolytic pathway.

1990 • 16 citations

Inhibition of ubiquitin-dependent proteolysis by des-Gly-Gly-ubiquitin: Implications for the mechanism of polyubiquitin synthesis

1989 • 15 citations