Glucagon desensitization of adenylate cyclase and stimulation of inositol phospholipid metabolism does not involve the inhibitory guanine nucleotide regulatory protein Gi, which is inactivated upon challenge of hepatocytes with glucagon

A simplification of the protein assay method of Lowry et al. which is more generally applicable

1977 • 8,485 citations

G PROTEINS: TRANSDUCERS OF RECEPTOR-GENERATED SIGNALS

1987 • 6,374 citations

Lithium amplifies agonist-dependent phosphatidylinositol responses in brain and salivary glands

1982 • 2,148 citations

Role of guanine nucleotide binding protein in the activation of polyphosphoinositide phosphodiesterase

1985 • 960 citations

Use of the liquid scintillation spectrometer for determining adenosine triphosphate by the luciferase enzyme

1969 • 898 citations

Protein kinase C phosphorylates the inhibitory guanine‐nucleotide‐binding regulatory component and apparently suppresses its function in hormonal inhibition of adenylate cyclase

1985 • 736 citations

Activation of two signal-transduction systems in hepatocytes by glucagon

1986 • 383 citations

Use of Isolated Liver Cells and Kidney Tubules in Metabolic Studies

1976 • 252 citations

Abolition of the expression of inhibitory guanine nucleotide regulatory protein Gi activity in diabetes

1987 • 232 citations

A role of the B-oligomer moiety of islet-activating protein, pertussis toxin, in development of the biological effects on intact cells.

1983 • 227 citations

Modulation of adenylate cyclase of human platelets by phorbol ester

1985 • 225 citations

The glucagon receptor of rat liver plasma membrane can couple to adenylate cyclase without activating it

1976 • 158 citations

Insulin and glucagon regulate the activation of two distinct membrane-bound cyclic AMP phosphodiesterases in hepatocytes

1983 • 156 citations

Guanine nucleotide inhibition of cyc- S49 mouse lymphoma cell membrane adenylyl cyclase.

1982 • 151 citations

The phorbol ester, TPA inhibits glucagon‐stimulated adenylate cyclase activity

1984 • 128 citations

Glucagon-induced heterologous desensitization of the MDCK cell adenylyl cyclase. Increases in the apparent levels of the inhibitory regulator (Ni).

1984 • 119 citations

Purification and properties of the insulin-stimulated cyclic AMP phosphodiesterase from rat liver plasma membranes

1981 • 100 citations

Treatment of intact hepatocytes with either the phorbol ester TPA or glucagon elicits the phosphorylation and functional inactivation of the inhibitory guanine nucleotide regulatory protein G_i

1989 • 93 citations

Conversion of adrenergic mechanism from an alpha- to a beta-type during primary culture of rat hepatocytes. Accompanying decreases in the function of the inhibitory guanine nucleotide regulatory component of adenylate cyclase identified as the substrate of islet-activating protein.

1984 • 90 citations

Insulin inhibits the cholera-toxin-catalysed ribosylation of a Mr-25000 protein in rat liver plasma membranes

1985 • 84 citations

Insulin, glucagon and the receptor-mediated control of cyclic AMP concentrations in liver

1986 • 84 citations

Challenge of hepatocytes by glucagon triggers a rapid modulation of adenylate cyclase activity in isolated membranes

1983 • 81 citations

The rapid desensitization of glucagon-stimulated adenylate cyclase is a cyclic AMP-independent process that can be mimicked by hormones which stimulate inositol phospholipid metabolism

1987 • 81 citations

Forskolin and ethanol both perturb the structure of liver plasma membranes and activate adenylate cyclase activity

1983 • 73 citations

Adenine nucleotides promote dissociation of pertussis toxin subunits.

1986 • 72 citations

Islet-activating protein blocks glucagon desensitization in intact hepatocytes

1984 • 59 citations

Differential effects of tryptophan on glucose synthesis in rats and guinea pigs

1978 • 54 citations

Human T lymphocyte mitogenesis in response to the B oligomer of pertussis toxin is associated with an early elevation in cytosolic calcium concentrations

1987 • 43 citations

Pertussis toxin can activate human platelets. Comparative effects of holotoxin and its ADP-ribosylating S1 subunit.

1987 • 43 citations

Multiple defects occur in the guanine nucleotide regulatory protein system in liver plasma membranes of obese (fa/fa) but not lean (Fa/Fa) zucker rats: Loss of functional G1 and abnormal Gs function

1989 • 41 citations

Cholera toxin mediated activation of adenylate cyclase in intact rat hepatocytes

1979 • 41 citations

Dual effects of pertussis toxin on lymphoid cells in culture

1986 • 34 citations

Pertussis toxin prevents homologous desensitization of adenylate cyclase in cultured renal epithelial cells.

1986 • 32 citations

Treatment of streptozotocin-diabetic rats with metformin restores the ability of insulin to inhibit adenylate cyclase activity and demonstrates that insulin does not exert this action through the inhibitory guanine nucleotide regulatory protein Gi

1988 • 30 citations

The phorbol ester TPA prevents the expression of both glucagon desensitisation and the glucagon‐mediated block of insulin stimulation of the peripheral plasma membrane cyclic AMP phosphodiesterase in rat hepatocytes

1985 • 29 citations

Resensitization of hepatocyte glucagon-stimulated adenylate cyclase can be inhibited when cyclic AMP phosphodiesterase inhibitors are used to elevate intracellular cyclic AMP concentrations to supraphysiological values

1988 • 15 citations

Membrane Structural/Functional Properties of Adipocytes from Normal and Streptozotocin-diabetic Rats

1984 • 14 citations