Analysis of the adenylate cyclase signalling system, and alterations induced by culture with insulin, in a novel SV40-DNA-immortalized hepatocyte cell line (P9 cells)

PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENT

1951 • 319,299 citations

A highly sensitive adenylate cyclase assay

1974 • 4,213 citations

Receptor-effector coupling by G proteins

1990 • 1,117 citations

The MAP kinase cascade is essential for diverse signal transduction pathways

1993 • 1,091 citations

Regulation of gene expression in adult rat hepatocytes cultured on a basement membrane matrix

1988 • 470 citations

Expression and function of IRS-1 in insulin signal transmission.

1992 • 331 citations

‘Crosstalk’: a pivotal role for protein kinase C in modulating relationships between signal transduction pathways

1991 • 328 citations

Rat hepatocyte primary cell cultures

1977 • 239 citations

Abolition of the expression of inhibitory guanine nucleotide regulatory protein Gi activity in diabetes

1987 • 232 citations

Modulation of functional activities in cultured rat hepatocytes

1983 • 219 citations

Expression of early genes of origin-defective mutants of simian virus 40.

1980 • 205 citations

The Regulation of Gluconeogenesis in Isolated Rat Liver Cells by Glucagon, Insulin, Dibutyryl Cyclic Adenosine Monophosphate, and Fatty Acids

1972 • 180 citations

Binding of 125I-labeled glucagon and glucagon-stimulated accumulation of adenosine 3‘:5‘-monophosphate in isolated intact rat hepatocytes. Evidence for receptor heterogeneity.

1978 • 127 citations

Hormonal regulation of Gi2 α-subunit phosphorylation in intact hepatocytes

1990 • 117 citations

Exchange of partners in glucagon receptor-adenylate cyclase complexes. Physical evidence for the independent, mobile receptor model

1977 • 113 citations

Changes in the form of Arrhenius plots of the activity of glucagon-stimulated adenylate cyclase and other hamster liver plasma-membrane enzymes occurring on hibernation

1978 • 106 citations

Diabetes-induced alterations in the expression, functioning and phosphorylation state of the inhibitory guanine nucleotide regulatory protein Gi-2 in hepatocytes

1990 • 95 citations

Development of opiate receptors and GTP-binding regulatory proteins in neonatal rat brain.

1987 • 94 citations

Transformation of rat hepatocytes by transfection with simian virus 40 DNA to yield proliferating differentiated cells.

1986 • 89 citations

Insulin inhibits the cholera-toxin-catalysed ribosylation of a Mr-25000 protein in rat liver plasma membranes

1985 • 84 citations

Challenge of hepatocytes by glucagon triggers a rapid modulation of adenylate cyclase activity in isolated membranes

1983 • 81 citations

The rapid desensitization of glucagon-stimulated adenylate cyclase is a cyclic AMP-independent process that can be mimicked by hormones which stimulate inositol phospholipid metabolism

1987 • 81 citations

Forskolin and ethanol both perturb the structure of liver plasma membranes and activate adenylate cyclase activity

1983 • 73 citations

Adenosine Diphosphate Ribosylation of G Proteins by Pertussis and Cholera Toxin in Isolated Membranes. Different Requirements for and Effects of Guanine Nucleotides and Mg²⁺

1987 • 61 citations

Islet-activating protein blocks glucagon desensitization in intact hepatocytes

1984 • 59 citations

Guanosine 5′-triphosphate and guanosine 5′-[βγ-imido]triphosphate effect a collision coupling mechanism between the glucagon receptor and catalytic unit of adenylate cyclase

1980 • 57 citations

Coupling of the α2-adrenergic receptor to the inhibitory G-protein Gi and adenylate cyclase in HT29 cells

1993 • 56 citations

Absence of the inhibitory effect of guanine nucleotides on adenylate cyclase activity in white adipocyte membranes of the ob/ob mouse. Effect of the ob gene.

1985 • 49 citations

Opioid peptides promote cholera-toxin-catalysed ADP-ribosylation of the inhibitory guanine-nucleotide-binding protein (Gi) in membranes of neuroblastoma x glioma hybrid cells

1988 • 44 citations

Evidence for Impaired Coupling of Receptors to Gi Protein in Adipocytes From Streptozocin-Induced Diabetic Rats

1991 • 43 citations

Diabetes‐induced changes in guanine‐nucleotide‐regulatory‐protein mRNA detected using synthetic oligonucleotide probes

1990 • 41 citations

Cholera toxin mediated activation of adenylate cyclase in intact rat hepatocytes

1979 • 41 citations

Okadaic acid identifies a phosphorylation/dephosphorylation cycle controlling the inhibitory guanine-nucleotide-binding regulatory protein Gi2

1991 • 41 citations

Changes in the phosphorylation state of the inhibitory guanine‐nucleotide‐binding protein G_i‐2 in hepatocytes from lean (Fa/Fa) and obese (fa/fa) Zucker rats

1990 • 35 citations

Functional alterations of G-proteins in diabetic rat retina: a possible explanation for the early visual abnormalities in diabetes mellitus

1992 • 33 citations

Alterations in G-protein expression and the hormonal regulation of adenylate cyclase in the adipocytes of obese (fa/fa) Zucker rats

1991 • 32 citations

The use of selective inhibitors and computer modelling to evaluate the role of specific high affinity cyclic AMP phosphodiesterases in the hormonal regulation of hepatocyte intracellular cyclic AMP concentrations

1990 • 31 citations

Gi-2 at the centre of an active phosphorylation/dephosphorylation cycle in hepatocytes: The fine-tuning of stimulatory and inhibitory inputs into adenylate cyclase in normal and diabetic states

1991 • 30 citations

Effects of Prostaglandins on Hepatic Adenylate Cyclase Activity and Cyclic Adenosine 3',5',-Monophosphate Content¹

1974 • 29 citations

Alterations in G-protein expression, Gi function and stimulatory receptor-mediated regulation of adipocyte adenylyl cyclase in a model of insulin-resistant diabetes with obesity

1992 • 28 citations

Molecular basis of insulin receptor function

1989 • 27 citations

Genetically acquired diabetes: adipocyte guanine nucleotide regulatory protein expression and adenylate cyclase regulation

1991 • 21 citations

Determination of G-protein levels, ADP-ribosylation by cholera and pertussis toxins and the regulation of adenylyl cyclase activity in liver plasma membranes from lean and genetically diabetic (db/db)

1991 • 15 citations

A mnemonical or negative-co-operativity model for the activation of adenylate cyclase by a common G-protein-coupled calcitonin-gene-related neuropeptide (CGRP)/amylin receptor

1993 • 15 citations

Glucagon‐Induced Refractoriness of Hepatocyte Adenylate Cyclase: Comparison of Homologous and Heterologous Components and Evidence Against a Role of cAMP

1989 • 12 citations