Insulin-stimulated Protein Tyrosine Phosphorylation in Intact Cells Evaluated by Giant Two-dimensional Gel Electrophoresis

The human insulin receptor cDNA: The structural basis for hormone-activated transmembrane signalling

1985 • 1,485 citations

Insulin Stimulates the Phosphorylation of the 95,000-Dalton Subunit of Its Own Receptor

1982 • 1,138 citations

Replacement of insulin receptor tyrosine residues 1162 and 1163 compromises insulin-stimulated kinase activity and uptake of 2-deoxyglucose

1986 • 1,100 citations

After Insulin Binds

1987 • 687 citations

Insulin stimulates tyrosine phosphorylation of the insulin receptor in a cell-free system

1982 • 680 citations

Insulin rapidly stimulates tyrosine phosphorylation of a Mr-185,000 protein in intact cells

1985 • 665 citations

Human insulin receptors mutated at the ATP-binding site lack protein tyrosine kinase activity and fail to mediate postreceptor effects of insulin.

1987 • 648 citations

Phosphorylation activates the insulin receptor tyrosine protein kinase.

1983 • 558 citations

Evidence for the involvement of vicinal sulfhydryl groups in insulin-activated hexose transport by 3T3-L1 adipocytes.

1985 • 512 citations

ENZYME AND METABOLIC INHIBITORS

1964 • 504 citations

A cascade of tyrosine autophosphorylation in the beta-subunit activates the phosphotransferase of the insulin receptor.

1988 • 467 citations

Insulin stimulation of phosphorylation of the beta subunit of the insulin receptor. Formation of both phosphoserine and phosphotyrosine.

1982 • 441 citations

Replacement of lysine residue 1030 in the putative ATP-binding region of the insulin receptor abolishes insulin- and antibody-stimulated glucose uptake and receptor kinase activity.

1987 • 399 citations

Mutation of the insulin receptor at tyrosine 960 inhibits signal transmission but does not affect its tyrosine kinase activity

1988 • 368 citations

Growth factor-stimulated protein phosphorylation in 3T3-L1 cells. Evidence for protein kinase C-dependent and -independent pathways.

1985 • 351 citations

Identification of multiple novel polypeptide substrates of the v-src, v-yes, v-fps, v-ros, and v-erb-B oncogenic tyrosine protein kinases utilizing antisera against phosphotyrosine.

1988 • 351 citations

Molecular cloning, characterization, and expression of a cDNA encoding the "80- to 87-kDa" myristoylated alanine-rich C kinase substrate: a major cellular substrate for protein kinase C.

1989 • 318 citations

Helper-independent transformation by unintegrated Harvey sarcoma virus DNA

1978 • 277 citations

Insulin-stimulated tyrosine phosphorylation of the insulin receptor in detergent extracts of human placental membranes. Comparison to epidermal growth factor-stimulated phosphorylation.

1982 • 254 citations

Identification of calmodulin-dependent protein kinase III and its major Mr 100,000 substrate in mammalian tissues.

1985 • 217 citations

Purification and characterization of Ca2+/calmodulin-dependent protein kinase I from bovine brain.

1987 • 212 citations

Tyrosine phosphorylation of common and specific sets of cellular proteins rapidly induced by insulin, insulin-like growth factor I, and epidermal growth factor in an intact cell.

1987 • 195 citations

Insulin Receptor: Structure and Function

1981 • 187 citations

Identification of c-fos sequences involved in induction by insulin and phorbol esters.

1988 • 184 citations

Insulin-activated tyrosine phosphorylation of a 15-kilodalton protein in intact 3T3-L1 adipocytes.

1987 • 181 citations

Regulation of Cell Growth and Transformation by Tyrosine-Specific Protein Kinases: The Search for Important Cellular Substrate Proteins

1983 • 164 citations

High-level expression of human insulin receptor cDNA in mouse NIH 3T3 cells.

1987 • 151 citations

Tyrosine Phosphorylation of Phospholipase C-II in Vitro by the Epidermal Growth Factor Receptor

1989 • 145 citations

Insulin-like growth factor I rapidly stimulates tyrosine phosphorylation of a Mr 185,000 protein in intact cells.

1987 • 125 citations

The glucose transporter in 3T3-L1 adipocytes is phosphorylated in response to phorbol ester but not in response to insulin.

1986 • 121 citations

Insulin and insulin-like growth factor-I stimulate a common endogenous phosphoprotein substrate (pp185) in intact neuroblastoma cells.

1987 • 120 citations

Serum, like phorbol esters, rapidly activates protein kinase C in intact quiescent fibroblasts.

1985 • 110 citations

British anti-lewisite

1946 • 106 citations

Effect of vanadate on the cellular accumulation of pp15, an apparent product of insulin receptor tyrosine kinase action.

1988 • 103 citations

The Insulin-Like Effect of Vanadate on Lipolysis in Rat Adipocytes Is Not Accompanied by an Insulin-Like Effect on Tyrosine Phosphorylation*

1989 • 98 citations

Insulin rapidly stimulates phosphorylation of a 46-kDa membrane protein on tyrosine residues as well as phosphorylation of several soluble proteins in intact fat cells.

1987 • 97 citations

Insulin stimulates phosphorylation of a 120-kDa glycoprotein substrate (pp120) for the receptor-associated protein kinase in intact H-35 hepatoma cells.

1987 • 84 citations

Effect of phenylarsine oxide on insulin-dependent protein phosphorylation and glucose transport in 3T3-L1 adipocytes.

1987 • 79 citations

British anti-lewisite

1946 • 74 citations

Identification of phosphorylated 422(aP2) protein as pp15, the 15-kilodalton target of the insulin receptor tyrosine kinase in 3T3-L1 adipocytes.

1988 • 72 citations

Characteristics of Insulin and Epidermal Growth Factor Stimulation of Receptor Autophosphorylation in Detergent Extracts of Rat Liver and Transplantable Rat Hepatomas*

1984 • 72 citations

Cell lines and peripheral blood leukocytes derived from individuals with chronic myelogenous leukemia display virtually identical proteins phosphorylated on tyrosine residues.

1987 • 72 citations

[15] Very-high-resolution two-dimensional electrophoretic separation of proteins on giant gels

1983 • 68 citations

Phosphorylation of the Insulin Receptor in Permeabilized Adipocytes Is Coupled to a Rapid Dephosphorylation Reaction

1989 • 67 citations

Insulin and growth factors stimulate the phosphorylation of a Mr-22000 protein in 3T3-L1 adipocytes

1983 • 67 citations

Insulin rapidly induces the biosynthesis of elongation factor 2

1989 • 49 citations

Comparative study of the tyrosine phosphorylation of proteins in Swiss 3T3 fibroblasts stimulated by a variety of mitogenic agents

1988 • 39 citations

Tyrosine Phosphorylation of pp185 by Insulin Receptor Kinase in a Cell-free System

1989 • 24 citations

Direct interaction of phenylarsine oxide with hexose transporters in isolated rat adipocytes

1988 • 19 citations

Evidence against insulin-stimulated phosphorylation of calmodulin in 3T3-L1 adipocytes

1989 • 18 citations

Chapter 16 Growth factor activation of protein kinase C-dependent and -independent pathways of protein phosphorylation in fibroblasts: relevance to activation of protein kinase C in neuronal tissues

1986 • 11 citations