Chapter 10 Transcription of Full-Length and Truncated mRNA Transcripts to Study Protein Translocation across the Endoplasmic Reticulum

Efficientin vitrosynthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter

1984 • 6,459 citations

An analysis of 5'-noncoding sequences from 699 vertebrate messenger RNAs

1987 • 5,139 citations

An Efficient mRNA‐Dependent Translation System from Reticulocyte Lysates

1976 • 3,994 citations

Functional messenger RNAs are produced by SP6in vitrotranscription of cloned cDNAs

1984 • 1,533 citations

70K heat shock related proteins stimulate protein translocation into microsomes

1988 • 1,276 citations

Functional dissection of a eukaryotic transcriptional activator protein, GCN4 of Yeast

1986 • 946 citations

Translocation of proteins across the endoplasmic reticulum III. Signal recognition protein (SRP) causes signal sequence-dependent and site-specific arrest of chain elongation that is released by microsomal membranes.

1981 • 807 citations

Structural gene identification and mapping by DNA-mRNA hybrid-arrested cell-free translation.

1977 • 543 citations

Synchronised transmembrane insertion and glycosylation of a nascent membrane protein

1977 • 529 citations

Injected anti-sense RNAs specifically block messenger RNA translation in vivo.

1985 • 410 citations

In vitro protein translocation across the yeast endoplasmic reticulum: ATP-dependent post-translational translocation of the prepro-α-factor

1986 • 332 citations

Alpha and Beta Globin Messenger Ribonucleic Acid

1971 • 329 citations

Secretory protein translocation in a yeast cell-free system can occur posttranslationally and requires ATP hydrolysis.

1986 • 315 citations

The signal recognition particle receptor mediates the GTP-dependent displacement of SRP from the signal sequence of the nascent polypeptide

1989 • 293 citations

A signal sequence receptor in the endoplasmic reticulum membrane

1987 • 291 citations

Translocation of secretory proteins across the microsomal membrane occurs through an environment accessible to aqueous perturbants

1985 • 277 citations

A novel transcription property of SP6 and T7 RNA polymerases: dependence on template structure

1985 • 267 citations

Gene within a gene: Nested Drosophila genes encode unrelated proteins on opposite DNA strands

1986 • 251 citations

The amino-terminal region of an imported mitochondrial precursor polypeptide can direct cytoplasmic dihydrofolate reductase into the mitochondrial matrix.

1984 • 236 citations

Bacteriophage SP6-specific RNA polymerase. I. Isolation and characterization of the enzyme.

1982 • 233 citations

Formation of a functional ribosome-membrane junction during translocation requires the participation of a GTP-binding protein.

1986 • 199 citations

Acyl-CoA oxidase contains two targeting sequences each of which can mediate protein import into peroxisomes.

1988 • 196 citations

Uncoupling Translocation from Translation: Implications for Transport of Proteins Across Membranes

1986 • 195 citations

Seventy-kilodalton heat shock proteins and an additional component from reticulocyte lysate stimulate import of M13 procoat protein into microsomes.

1988 • 187 citations

Secretion in yeast: translocation and glycosylation of prepro-α-factor in vitro can occur via an ATP-dependent post-translational mechanism

1986 • 185 citations

The human glucose transporter can insert posttranslationally into microsomes

1986 • 176 citations

Protein translocation across the endoplasmic reticulum membrane: identification by photocross-linking of a 39-kD integral membrane glycoprotein as part of a putative translocation tunnel.

1989 • 167 citations

Translation arrest by oligodeoxynucleotides complementary to mRNA coding sequences yields polypeptides of predetermined length

1986 • 142 citations

Non-hydrophobic extracytoplasmic determinant of stop transfer in the prion protein

1990 • 132 citations

Formation of the 3′ end of histone mRNA by post-transcriptional processing

1984 • 129 citations

Preparation of capped RNA transcripts using T7 RNA polymerase

1986 • 122 citations

Utilization of bacteriophage T7 late promoters in recombinant plasmids during infection

1981 • 120 citations

The preparation and characterization of a cell-free system from Saccharomyces cerevisiae that translates natural messenger ribonucleic acid.

1979 • 115 citations

Import of frog prepropeptide GLa into microsomes requires ATP but does not involve docking protein or ribosomes.

1987 • 111 citations

A former amino terminal signal sequence engineered to an internal location directs translocation of both flanking protein domains.

1985 • 98 citations

Access of proteinase K to partially translocated nascent polypeptides in intact and detergent-solubilized membranes.

1989 • 88 citations

Assembly of the Alu domain of the signal recognition particle (SRP): dimerization of the two protein components is required for efficient binding to SRP RNA.

1990 • 85 citations

The mode of action of alpha sarcin and a novel assay of the puromycin reaction

1978 • 77 citations

Post-translational insertion of fragment of the glucose transporter into microsomes requires phosphoanhydride bond cleavage

1986 • 75 citations

The ATP requiring step in assembly of M13 procoat protein into microsomes is related to preservation of transport competence of the precursor protein.

1987 • 73 citations

Evidence for a two-step mechanism involved in assembly of functional signal recognition particle receptor.

1989 • 64 citations

Signal recognition particle mediates the insertion of a transmembrane protein which has a cytoplasmic NH2 terminus.

1986 • 58 citations

A stop transfer sequence recognizes receptors for nascent chain translocation across the endoplasmic reticulum membrane

1986 • 54 citations

Integration of membrane proteins into the endoplasmic reticulum requires GTP.

1988 • 48 citations

Prepro-carboxypeptidase Y and a truncated form of pre-invertase, but not full-length pre-invertase, can be posttranslationally translocated across microsomal vesicle membranes from Saccharomyces cerevisiae.

1988 • 45 citations

Guanosine triphosphate promotes the post-translational integration of opsin into the endoplasmic reticulum membrane.

1988 • 42 citations

Synthetic oligonucleotide tails inhibitin vitroandin vivotranslation of SP6 transcripts of maize zein cDNA clones

1986 • 40 citations

Observations of the cosmic background radiation near the double quasar 1146 + 111B,C

1986 • 33 citations

Ribosome‐Protected Fragments from Sindbis 42‐S and 26‐S RNAs

1979 • 32 citations