Chapter 10 Use of Oligonucleotides for Antisense Experiments in Xenopus laevis Oocytes

Oogenesis in Xenopus laevis (Daudin). I. Stages of oocyte development in laboratory maintained animals

1972 • 1,861 citations

Dominoes and Clocks: the Union of Two Views of the Cell Cycle

1989 • 750 citations

Characterization of oligonucleotide transport into living cells.

1989 • 739 citations

Function of c-mos proto-oncogene product in meiotic maturation in Xenopus oocytes

1988 • 617 citations

U2 as well as U1 small nuclear ribonucleoproteins are involved in premessenger RNA splicing

1985 • 568 citations

Multiple factors including the small nuclear ribonucleoproteins U1 and U2 are necessary for Pre-mRNA splicing in vitro

1985 • 442 citations

Translation of cyclin mRNA is necessary for extracts of activated Xenopus eggs to enter mitosis

1989 • 438 citations

Injected anti-sense RNAs specifically block messenger RNA translation in vivo.

1985 • 410 citations

The 5′ terminus of the RNA moiety of U1 small nuclear ribonucleoprotein particles is required for the splicing of messenger RNA precursors

1984 • 404 citations

Nuclear segregation of U2 snRNA requires binding of specific snRNP proteins

1985 • 307 citations

Changes in somatic cell nuclei inserted into growing and maturing amphibian oocytes

1968 • 303 citations

Comparative inhibition of rabbit globin mRNA translation by modified antisense oligodeoxynucleotides

1989 • 238 citations

Selective elimination of mRNAs in vivo: complementary oligodeoxynucleotides promote RNA degradation by an RNase H-like activity.

1987 • 225 citations

Oligodeoxynucleotide stability in subcellular extracts and culture media

1986 • 224 citations

Pre-mRNA splicing in vitro requires intact U4/U6 small nuclear ribonucleoprotein

1986 • 222 citations

In vivo disruption of Xenopus U3 snRNA affects ribosomal RNA processing.

1990 • 214 citations

In vivo splicing products of the rabbit β-globin pre-mRNA

1984 • 209 citations

U1, U2, and U4/U6 small nuclear ribonucleoproteins are required for in vitro splicing but not polyadenylation

1986 • 192 citations

Enzymatic amplification of translation inhibition of rabbit β-globin mRNA mediated by anti-messenger oligodeoxynucleotides covalently linked to intercalating agents

1987 • 164 citations

The stability, toxicity and effectiveness of unmodified and phosphorothioate antisense oligodeoxynucleotides in Xenopus oocytes and embryos

1990 • 156 citations

Unraveling of mitotic control mechanisms

1988 • 150 citations

Rate of degradation of [α]- and [β]-oligodeoxynucleotides inXenopusoocytes. Implications for anti-messenger strategies

1987 • 143 citations

Antisense oligonucleotide-directed cleavage of mRNA in Xenopus oocytes and eggs.

1988 • 136 citations

In vitro assembly of yeast U6 snRNP: a functional assay.

1989 • 134 citations

Techniques for using antisense oligodeoxyribonucleotides to study gene expression

1988 • 130 citations

Domains of U4 and U6 snRNAs required for snRNP assembly and splicing complementation in Xenopus oocytes.

1990 • 127 citations

Antimessenger oligodeoxyribonucleotides: an alternative to antisense RNA for artificial regulation of gene expression — a review

1988 • 123 citations

Quantitative hybridization-arrant of mRNA InXenopusoocytes using single-tiranded coanplementary DNA or ollgonudeotlde probes

1985 • 111 citations

The cyclin B2 component of MPF is a substrate for the c-mosxe proto-oncogene product

1990 • 105 citations

Targeted degradation of mRNA inXenopusoocytes and embryos directed by modified oligonucleotides: studies of An2 and cyclin in embryogenesis

1990 • 103 citations

Functional analysis of mutant Xenopus U2 snRNAs

1989 • 103 citations

Antisense strategies in cell and developmental biology

1990 • 98 citations

Splicing pathways of SV40 mRNAs in X. laevis oocytes differ in their requirements for snRNPs

1984 • 98 citations

Developmental expression of the protein product of Vg1, a localized maternal mRNA in the frog Xenopus laevis.

1989 • 95 citations

In vitro reconstitution of functional yeast U2 snRNPs.

1989 • 91 citations

Post-transcriptional processing of simian virus 40 late transcripts in injected frog oocytes

1983 • 88 citations

Phosphoroselenoate oligodeoxynucleotides: synthesis, physico-chemical characterization, anti-sense inhibitory properties and anti-HIV activity

1989 • 73 citations

Positive and negative regulation of the gene for transcription factor IIIA in Xenopus laevis oocytes.

1989 • 70 citations

Ha-rasVal-12,Thr-59 activates S6 kinase and p34cdc2 kinase in Xenopus oocytes: evidence for c-mosxe-dependent and -independent pathways.

1990 • 66 citations

Chromatin Assembly and Transcription in Eggs and Oocytes of Xenopus laevis

1978 • 64 citations

U2 snRNA sequences that bind U2-specific proteins are dispensable for the function of U2 snRNP in splicing.

1989 • 64 citations

Axis and germ line deficiencies caused by u.v. irradiation of Xenopus oocytes cultured in vitro

1987 • 62 citations

Assembly of Functional U1 and U2 Human-Amphibian Hybrid snRNPs in Xenopus laevis oocytes

1988 • 60 citations

Multiple domains of U1 snRNA, including U1 specific protein binding sites, are required for splicing.

1990 • 59 citations

Effects of the v-mos oncogene on Xenopus development: meiotic induction in oocytes and mitotic arrest in cleaving embryos.

1990 • 57 citations

Antisense oligodeoxyribonucleotide-directed cleavage of maternal mRNA in Xenopus oocytes and embryos

1988 • 53 citations

Destruction of a translationally controlled mRNA in Xenopus oocytes delays progesterone-induced maturation.

1988 • 48 citations

Nonspecific effects of oligodeoxynucleotide injection in Xenopus oocytes: a reevaluation of previous D7 mRNA ablation experiments

1990 • 43 citations

Specific Block of Calcium Channel Expression by a Fragment of Dihydropyridine Receptor cDNA

1989 • 42 citations

Comparison of exogenous energy sources for in vitro maintenance of follicle cell-freeXenopus laevis oocytes

1976 • 40 citations

Discrimination of heterogenous mRNAs encoding strychnine-sensitive glycine receptors in Xenopus oocytes by antisense oligonucleotides.

1989 • 39 citations

Assembly of the α-subunit of Torpedo californica Na+/K+-ATPase with its pre-existing β-subunit in Xenopus oocytes

1990 • 38 citations

Requirement of ets -2 Expression for Xenopus Oocyte Maturation

1990 • 38 citations

The maternal store of the xlgv7 mRNA in full-grown oocytes is not required for normal development in Xenopus

1989 • 36 citations

Microinjected oligonucleotides complementary to the alpha-sarcin loop of 28 S RNA abolish protein synthesis in Xenopus oocytes.

1990 • 36 citations

Kinetics of Accumulation and Processing of Simian Virus 40 RNA inXenopus laevisOocytes Injected with Simian Virus 40 DNA

1982 • 33 citations

Specific inhibition of endogenous β-tubulin synthesis inXenopusoocytes by anti-messenger oligodeoxynucleotides

1988 • 29 citations

Using the Xenopus Oocyte System for Expression and Cloning of Neuroreceptors and Channels

1989 • 28 citations

Effects of oligo sequence and chemistry on the efficiency of oligodeoxyribonucleotide-mediated mRNA cleavage

1990 • 25 citations

Oligonudeotide-targeted degradation of U1 and U2 snRNAs reveals differential interactions of simian virus 40 pre-mRNAs with snRNPs

1989 • 18 citations

Identification of G protein‐coupled receptors by RNase H‐mediated hybrid depletion using Xenopus laevis oocytes as expression system

1990 • 13 citations

In vivophotocrosslinking reveals that transcription factor binding to the mammalian ATF recognitioi sequence is required for E1A-induced transactivation in injectedXenopus laevisoocytes

1989 • 13 citations

Regulation of simian virus 40 gene expression in Xenopus laevis oocytes.

1985 • 11 citations