Isolation and Characterization of Human Vascular Endothelial Cells with Application to Studies of the Subendothelial Matrix

A Film Detection Method for Tritium‐Labelled Proteins and Nucleic Acids in Polyacrylamide Gels

1974 • 8,693 citations

Culture of Human Endothelial Cells Derived from Umbilical Veins. IDENTIFICATION BY MORPHOLOGIC AND IMMUNOLOGIC CRITERIA

1973 • 6,904 citations

Use of a mixture of proteinase-free collagenases for the specific assay of radioactive collagen in the presence of other proteins

1971 • 1,396 citations

Migration and proliferation of endothelial cells in preformed and newly formed blood vessels during tumor angiogenesis

1977 • 1,286 citations

Identification and isolation of endothelial cells based on their increased uptake of acetylated-low density lipoprotein.

1984 • 1,249 citations

NEW CYTOPLASMIC COMPONENTS IN ARTERIAL ENDOTHELIA

1964 • 1,062 citations

Angiogenesis in vitro

1980 • 1,041 citations

[28] Enzyme immunoassay ELISA and EMIT

1980 • 1,014 citations

Isolation and characterization of angiogenin, an angiogenic protein from human carcinoma cells

1985 • 911 citations

HUMAN VASCULAR ENDOTHELIAL CELLS IN CULTURE

1974 • 839 citations

Human Endothelial Cells: Use of Heparin in Cloning and Long-Term Serial Cultivation

1983 • 801 citations

Long-term culture of capillary endothelial cells.

1979 • 780 citations

Immunolocalization of von Willebrand protein in Weibel-Palade bodies of human endothelial cells

1982 • 698 citations

Role of Cyclic Nucleotides in Growth Control

1975 • 692 citations

In vitro rapid organization of endothelial cells into capillary-like networks is promoted by collagen matrices.

1983 • 616 citations

Capillary endothelial cell cultures: phenotypic modulation by matrix components.

1983 • 611 citations

Ulex europaeus I lectin as a marker for vascular endothelium in human tissues.

1982 • 573 citations

Basal lamina scaffold-anatomy and significance for maintenance of orderly tissue structure.

1974 • 551 citations

Purification of the fibroblast growth factor activity from bovine brain.

1978 • 498 citations

Tumor-promoting phorbol esters induce angiogenesis in vitro

1985 • 470 citations

Biochemistry and Metabolism of Basement Membranes

1979 • 468 citations

Clonal growth of bovine vascular endothelial cells: fibroblast growth factor as a survival agent.

1976 • 423 citations

Selection and characterization of bovine aortic endothelial cells

1978 • 417 citations

Factors involved in the modulation of cell proliferation in vivo and in vitro: The role of fibroblast and epidermal growth factors in the proliferative response of mammalian cells

1978 • 360 citations

Serial propagation of human endothelial cells in vitro.

1981 • 348 citations

Stimulation of corneal endothelial cell proliferation in vitro by fibroblast and epidermal growth factors

1977 • 335 citations

Endothelial cell proliferation during angiogenesis. In vitro modulation by basement membrane components.

1986 • 277 citations

Evidence for the origin of Kaposi's sarcoma from lymphatic endothelium.

1985 • 252 citations

Organizational behavior of human umbilical vein endothelial cells.

1982 • 238 citations

Primary culture of capillary endothelium from rat brain

1981 • 234 citations

The viability of cells grown or centrifuged in a new density gradient medium, Percoll(TM)

1977 • 225 citations

Endothelial cell mitogens derived from retina and hypothalamus: biochemical and biological similarities.

1984 • 224 citations

Destruction of extracellular matrices containing glycoproteins, elastin, and collagen by metastatic human tumor cells.

1980 • 221 citations

Simultaneous synthesis of types I and III collagen by fibroblasts in culture.

1976 • 219 citations

Ulex europaeus I Lectin as a Marker for Tumors Derived from Endothelial Cells

1983 • 211 citations

Isolation and culture of microvascular endothelium from human adipose tissue.

1983 • 207 citations

Endothelial cell-matrix interactions: in vitro models of angiogenesis.

1986 • 205 citations

Culture of retinal capillary cells using selective growth media

1983 • 192 citations

Pathobiology of the Endothelial Cell

1982 • 190 citations

Fine structure of vascular endothelium in culture

1975 • 181 citations

Isolation and Growth of Endothelial Cells from the Microvessels of the Newborn Human Foreskin in Cell Culture

1980 • 177 citations

Composition of the von Willebrand factor storage organelle (Weibel-Palade body) isolated from cultured human umbilical vein endothelial cells.

1987 • 176 citations

Comparison of Ulex europaeus I lectin and factor VIII-related antigen in vascular lesions.

1984 • 176 citations

Mast cell granules cause proliferation of human microvascular endothelial cells.

1986 • 168 citations

Angiogenic activity from bovine retina: partial purification and characterization.

1981 • 154 citations

The isolation and culture of capillary endothelium from epididymal fat

1975 • 150 citations

Secretory phenotypes of endothelial cells in culture: comparison of aortic, venous, capillary, and corneal endothelium.

1981 • 146 citations

A unique family of endothelial cell polypeptide mitogens: the antigenic and receptor cross-reactivity of bovine endothelial cell growth factor, brain-derived acidic fibroblast growth factor, and eye-derived growth factor-II.

1985 • 146 citations

Control of proliferation of bovine vascular endothelial cells

1977 • 139 citations

Isolation and Characterization of Brain Endothelial Cells: Morphology and Enzyme Activity

1980 • 137 citations

Collagen synthesis by bovine aortic endothelial cells in culture

1979 • 137 citations

Replication of Human Endothelial Cells in Culture

1973 • 137 citations

Cerebral microvessels and derived cells in tissue culture: Isolation and preliminary characterization

1979 • 133 citations

Effects Of Culture Conditions On The Proliferation, Morphology And Migration Of Bovine Aortic Endothelial Cells

1983 • 131 citations

Pericellular Matrix in Malignant Transformation

1982 • 114 citations

Biosynthetic and structural properties of endothelial cell type VIII collagen.

1983 • 104 citations

Long-term culture of human endothelial cells

1983 • 96 citations

Primary culture of microvascular endothelial cells from bovine retina

1982 • 91 citations

Bovine brain and pituitary fibroblast growth factors: comparison of their abilities to support the proliferation of human and bovine vascular endothelial cells.

1983 • 90 citations

Human dermal microvascular endothelial cells in vitro: Effect of cyclic AMP on cellular morphology and proliferation rate

1981 • 88 citations

Human dermal microvascular endothelial cells: An improved method for tissue culture and a description of some singular properties in culture

1985 • 87 citations

Primary cultures of endothelial cells of the rat liver

1982 • 81 citations

Protein-derivatised glass coverslips for the study of cell-to-substratum adhesion

1981 • 80 citations

Growth factors and their action in vivo and in vitro

1983 • 78 citations

Tumor angiogenic factor. Purification from the Walker 256 rat tumor.

1981 • 76 citations

Synthesis of extracellular matrix glycoproteins by cultured microvascular endothelial cells isolated from the dermis of neonatal and adult skin

1985 • 72 citations

Importance of a collagen substratum for stimulation of capillary endothelial cell proliferation by tumour angiogenesis factor

1979 • 70 citations

Isolation and characterization of endothelial cells from the heart microvasculature

1978 • 70 citations

Basal lamina formation by cultured microvascular endothelial cells.

1984 • 69 citations

The formation of capillary-like tubes by calf aortic endothelial cells grown in vitro

1983 • 67 citations

The isolation and purification of two anionic endothelial cell growth factors from human brain

1984 • 66 citations

Isolation and long-term serial cultivation of endothelial cells from the microvessels of the adult human dermis

1983 • 66 citations

Possible contribution of basement membrane to the structural rigidity of blood capillaries

1975 • 66 citations

Clonal heterogeneity in colony stimulating factor production by murine T lymphocytes

1985 • 65 citations

Identification of UEA I-binding surface glycoproteins of cultured human endothelial cells

1983 • 64 citations

Endothelial cells from umbilical vein and a hemangioendothelioma secrete basement membrane largely to the exclusion of interstitial procollagens.

1982 • 63 citations

Selective Degradation of Basement Membrane Macromolecules by Metastatic Melanoma Cells<xref ref-type="fn" rid="FN2">2</xref>

1984 • 63 citations

[4] Preparation and characterization of procollagens and procollagen-collagen intermediates

1982 • 56 citations

Type IV collagen synthesis by cultured human microvascular endothelial cells and its deposition into the subendothelial basement membrane

1985 • 55 citations

Angiotensin I-converting enzyme localization on cultured fibroblasts by immunofluorescence

1982 • 45 citations

Retinal vessels: proliferation of endothelium in vitro.

1979 • 43 citations

Properties of cultured endothelium from adult human vessels.

1984 • 42 citations

Cultivation of microvascular endothelial cells from human preputial skin

1980 • 39 citations

Extracellular matrix interactions with the apical surface of vascular endothelial cells

1985 • 35 citations

Studies on Atherogenesis and Corneal Transplantation Using Cultured Vascular and Corneal Endothelia

1979 • 34 citations

A mitogenic factor for endothelial cells obtained from mouse secondary mixed leukocyte cultures.

1986 • 34 citations

Expression of factor VIII-related antigen and ulex lectin binding sites in endothelial cells during long-term culture

1982 • 34 citations

A reevaluation of the response of human umbilical vein endothelial cells to certain growth factors

1983 • 30 citations

Regulation of angiotensin I‐converting enzyme activity in serially cultivated bovine endothelial cells

1985 • 27 citations

Collagen synthesis by cloned pulmonary artery endothelial cells

1984 • 12 citations

Angiogenesis in Vitro: Implications for Tumor Biology

1982 • 10 citations

Fibroblast Growth Factor: Molecular and Biological Properties

1987 • 8 citations

Synthesis of basement membrane-specific macromolecules by cultured human microvascular endothelial cells isolated from skin of diabetic and nondiabetic subjects

1986 • 5 citations