Requirement of a polypyrimidine tract for trans-splicing in trypanosomes: discriminating the PARP promoter from the immediately adjacent 3′ splice acceptor site.

Analysis of enzymatically amplified β-globin and HLA-DQα DNA with allele-specific oligonucleotide probes

1986 • 1,863 citations

Transcriptional Control Signals of a Eukaryotic Protein-Coding Gene

1982 • 1,406 citations

Splicing of Messenger RNA Precursors

1987 • 919 citations

PRE-mRNA SPLICING

1986 • 673 citations

Fluorescence-activated cell analysis and sorting of viable mammalian cells based on beta-D-galactosidase activity after transduction of Escherichia coli lacZ.

1988 • 487 citations

Evidence for Trans splicing in trypanosomes

1986 • 486 citations

Stable transfection of the human parasite Leishmania major delineates a 30-kilobase region sufficient for extrachromosomal replication and expression.

1990 • 450 citations

Trans splicing of nuclear pre-mRNAs

1990 • 383 citations

Identification of a novel Y branch structure as an intermediate in trypanosome mRNA processing: Evidence for Trans splicing

1986 • 354 citations

The genes and transcripts of an antigen gene expression site from T. brucei

1989 • 257 citations

Gene replacement in parasitic protozoa

1990 • 238 citations

Three small RNAs within the 10 kb trypanosome rRNA transcription unit are analogous to Domain VII of other eukaryotic 28S rRNAs

1986 • 237 citations

The anatomy and transcription of a telomeric expression site for variant-specific surface antigens in T. brucei

1987 • 218 citations

Trans splicing involves a novel form of small nuclear ribonucleoprotein particles

1988 • 210 citations

RNA splicing is required to make the messenger RNA for a variant surface antigen in trypanosomes

1982 • 183 citations

Cloning and sequencing of the ornithine decarboxylase gene from Trypanosoma brucei. Implications for enzyme turnover and selective difluoromethylornithine inhibition.

1987 • 181 citations

Targeted insertion of the neomycin phosphotransferase gene into the tubulin gene cluster of Trypanosoma brucei

1990 • 177 citations

Coordinate transcription of variant surface glycoprotein genes and an expression site associated gene family in Trypanosoma brucei

1985 • 171 citations

Calmodulin genes in trypanosomes are tandemly repeated and produce multiple mRNAs with a common 5' leader sequence.

1985 • 162 citations

Stable expression of the bacterial neor gene in Leishmania enriettii

1990 • 159 citations

Expression of a Bacterial Gene in a Trypanosomatid Protozoan

1989 • 149 citations

Trypanosoma brucei: constitutive activity of the VSG and procyclin gene promoters.

1990 • 140 citations

Transcription of the procyclic acidic repetitive protein genes of Trypanosoma brucei.

1990 • 137 citations

Homologous Recombination and Stable Transfection in the Parasitic Protozoan Trypanosoma brucei

1990 • 135 citations

Characterization of the gene for the microbody (glycosomal) triosephosphate isomerase of Trypanosoma brucei.

1986 • 135 citations

Destruction of U2, U4, or U6 small nuclear RNA blocks Trans splicing in trypanosome cells

1990 • 131 citations

Post-transcriptional control of the differential expression of phosphoglycerate kinase genes in Trypanosoma brucei

1988 • 130 citations

Stable Transfection of the Human Parasite Leishmania major Delineates a 30-Kilobase Region Sufficient for Extrachromosomal Replication and Expression

1990 • 127 citations

Isolation and sequence of four small nuclear U RNA genes of Trypanosoma brucei subsp. brucei: identification of the U2, U4, and U6 RNA analogs.

1989 • 122 citations

Alpha-amanitin resistant transcription of protein coding genes in insect and bloodstream form Trypanosoma brucei.

1989 • 117 citations

Transfection of Leishmania enriettii and expression of chloramphenicol acetyltransferase gene.

1989 • 116 citations

Trypanosoma brucei contains two RNA polymerase II largest subunit genes with an altered C-terminal domain

1989 • 116 citations

Procyclic acidic repetitive protein (PARP) genes located in an unusually small alpha-amanitin-resistant transcription unit: PARP promoter activity assayed by transient DNA transfection of Trypanosoma brucei.

1990 • 112 citations

Structure and regulated expression of genes encoding fructose biphosphate aldolase in Trypanosoma brucei.

1985 • 110 citations

Conserved Sequences and Transcription of the hsp70 Gene Family in Trypanosoma brucei

1986 • 108 citations

In trypanosomes the homolog of the largest subunit of RNA polymerase II is encoded by two genes and has a highly unusual C-terminal domain structure

1989 • 100 citations

RNA polymerase I can mediate expression of CAT and neo protein-coding genes in Trypanosoma brucei.

1991 • 99 citations

Stable integrative transformation of Trypanosoma brucei that occurs exclusively by homologous recombination.

1991 • 94 citations

Discontinuous transcription and splicing in trypanosomes

1986 • 93 citations

Trans splicing in trypanosomes — archaism or adaptation?

1989 • 93 citations

In vivo labelling of intermediates in the discontinuous synthesis of mRNAs in Trypanosoma brucei.

1987 • 92 citations

Spliced leader RNA sequences can substitute for the essential 5′ end of U1 RNA during splicing in a mammalian in vitro system

1990 • 92 citations

A 5' spliced leader is added in trans to both alpha- and beta-tubulin transcripts in Trypanosoma brucei.

1985 • 88 citations

The U2 RNA analogue ofTrypanosoma brucei gambiense: implications for a splicing mechanism in trypanosomes

1986 • 86 citations

VSG gene 118 is transcribed from a cotransposed pol I-like promoter

1987 • 82 citations

A new U2 RNA secondary structure provided by phylogenetic analysis of trypanosomatid U2 RNAs.

1990 • 47 citations

Mapping of Branch Sites in trans-Spliced Pre-mRNAs of Trypanosoma brucei

1989 • 46 citations

The genes encoding fructose bisphosphate aldolase inTrypanosoma bruceiare interspersed with unrelated genes

1990 • 46 citations

Procyclic Acidic Repetitive Protein (PARP) Genes Located in an Unusually Small α-Amanitin-Resistant Transcription Unit: PARP Promoter Activity Assayed by Transient DNA Transfection of Trypanosoma brucei

1990 • 46 citations

Alternate trans Splicing in Trypanosoma equiperdum: Implications for Splice Site Selection

1988 • 43 citations

Isolation and Sequence of Four Small Nuclear U RNA Genes of Trypanosoma brucei subsp. brucei: Identification of the U2, U4, and U6 RNA Analogs

1989 • 40 citations

A Similar Gene Is Shared by Both the Variant Surface Glycoprotein and Procyclin Gene Transcription Units of Trypanosoma brucei

1991 • 37 citations

Trypanosome trans-splicing utilizes 2′-5′ branches and a corresponding debranching activity.

1988 • 37 citations

Transcription of the Procyclic Acidic Repetitive Protein Genes of Trypanosoma brucei

1990 • 35 citations

TheTrypanosoma bruceiprotein phosphatase gene: polycistronic transcription with the RNA polymerase II largest subunit gene

1990 • 28 citations

Physical identification of branched intron side-products of splicing in Trypanosoma brucei.

1988 • 26 citations