[18] Purification of integral membrane proteins

A SIMPLE METHOD FOR THE ISOLATION AND PURIFICATION OF TOTAL LIPIDES FROM ANIMAL TISSUES

1957 • 63,238 citations

A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATION

1959 • 47,127 citations

Phase separation of integral membrane proteins in Triton X-114 solution.

1981 • 3,366 citations

Partition of Cell Particles and Macromolecules

1973 • 2,047 citations

Isolation of intracellular membranes by means of sodium carbonate treatment: application to endoplasmic reticulum.

1982 • 1,765 citations

A simple procedure for removal of triton X-100 from protein samples

1973 • 857 citations

Selective solubilization of proteins and phospholipids from red blood cell membranes by nonionic detergents

1973 • 687 citations

Reconstitution and Purification of the D-Glucose Transport Protein from Human Erythrocytes

1977 • 652 citations

Reconstitution and purification of the D-glucose transporter from human erythrocytes.

1977 • 558 citations

Glycoproteins: Isolation from Cell Membranes with Lithium Diiodosalicylate

1971 • 543 citations

Studies on the electron transfer system

1966 • 288 citations

Enhanced molecular diffusibility in muscle membrane blebs: release of lateral constraints.

1982 • 255 citations

Purification and properties of a dicyclohexylcarbodiimide-sensitive adenosine triphosphatase from a thermophilic bacterium.

1975 • 173 citations

Activities of lectins and their immobilized derivatives in detergent solutions. Implications on the use of lectin affinity chromatography for the purification of membrane glycoproteins

1977 • 160 citations

Monoclonal Antibodies to Ia Antigens from Rat Thymus: Cross Reactions with Mouse and Human and Use in Purification of Rat Ia Glycoproteins

1979 • 159 citations

Hydrophobic interaction chromatography on noncharged sepharose® derivatives

1975 • 132 citations

A method for the direct demonstration of the lectin-binding components of the human erythrocyte membrane

1976 • 126 citations

Purification of immunologically active HLA-A and -B antigens by a series of monoclonal antibody columns.

1979 • 97 citations

Abnormal carbohydrate composition of the major penetrating membrane protein of En(a-) human erythrocytes

1976 • 94 citations

Partition between polymer phases

1978 • 93 citations

The proteins of the erythrocyte membrane obtained by solubilization with aqueous pyridine solution

1968 • 92 citations

Purification of stabilized band 3 protein of the human erythrocyte membrane and its reconstitution into liposomes

1981 • 89 citations

Solubilization and purification of rat liver 5′-nucleotidase by use of a zwitterionic detergent and a monoclonal-antibody immunoadsorbent

1982 • 72 citations

Isolation of lipid-free plasma membrane proteins by gel filtration on Sephadex LH-20 using 2-chloroethanol-water as solvent

1967 • 59 citations

The distribution of blood-group antigens on butanol extraction of human erythrocyte ‘ghosts’

1974 • 55 citations

Purification and characterization of an acetylcholine receptor from mammalian skeletal muscle

1977 • 54 citations

Reconstitution of membranes by recombining proteins and lipids derived from erythrocyte stroma

1970 • 53 citations

Separation of particles and macromolecules by phase partition

1977 • 47 citations

Estimation of molecular weights of membrane proteins in the presence of SDS by low-angle laser light scattering combined with high-performance porous silica gel chromatography confirmation of the trimer structure of porin of the E. coli outer membrane

1982 • 44 citations

Electrophoresis: A new preparative desorption technique in affinity chromatography (and immunoadsorption)

1978 • 33 citations

Chromatographic Separation of Membrane Proteins on Lipophilic Ion Exchange Resins

1970 • 32 citations

Separation of membrane components by partition in detergent-containing polymer phase systems

1981 • 23 citations

Phenyl-Sepharose chromatography of membrane proteins solubilized in Triton X-100

1981 • 20 citations

Protein chromatography on adsorbents with hydrophobic and ionic groups. Chromatography of dodecyl sulphate-solubilized proteins of the human erythrocyte membrane on N-(3-carboxypropionyl)aminodecyl-sepharose

1976 • 17 citations

Blood‐Group Serology of Fractions Obtained from the Human Erythrocyte Membrane

1974 • 15 citations

Isolation of human platelet and red blood cell plasma membrane proteins by preparative detergent electrophoresis

1979 • 13 citations

The solubilization and fractionation of human erythrocyte membrane proteins

1972 • 12 citations

Solubilization of Escherichia coli membrane proteins by aprotic solvents

1977 • 11 citations

Isolation of platelet plasma membrane proteins by affinity chromatography

1981 • 3 citations