Human lymphocyte Hybridomas and Monoclonal Antibodies

Continuous cultures of fused cells secreting antibody of predefined specificity

1975 • 17,037 citations

Selection of Hybrids from Matings of Fibroblasts in vitro and Their Presumed Recombinants

1964 • 2,108 citations

A New Mouse Myeloma Cell Line that Has Lost Immunoglobulin Expression but Permits the Construction of Antibody-Secreting Hybrid Cell Lines

1979 • 1,885 citations

Cellular localization of an Epstein‐Barr virus (EBV)‐associated complement‐fixing antigen in producer and non‐producer lymphoblastoid cell lines

1973 • 1,718 citations

Electric field-mediated fusion and related electrical phenomena

1982 • 1,008 citations

Chimeric human antibody molecules: mouse antigen-binding domains with human constant region domains.

1984 • 1,002 citations

Release of Infectious Epstein-Barr Virus by Transformed Marmoset Leukocytes

1973 • 960 citations

Fusion between immunoglobulin‐secreting and nonsecreting myeloma cell lines

1976 • 729 citations

Electric field-induced cell-to-cell fusion

1982 • 580 citations

Introduction of new genetic material into pluripotent haematopoietic stem cells of the mouse

1984 • 527 citations

Polyspecificity of Monoclonal Lupus Autoantibodies Produced by Human-Human Hybridomas

1983 • 453 citations

A vector that replicates as a plasmid and can be efficiently selected in B-lymphoblasts transformed by Epstein-Barr virus.

1985 • 445 citations

Immunoglobulin gene expression in transformed lymphoid cells.

1983 • 400 citations

EB virus-induced B lymphocyte cell lines producing specific antibody

1977 • 390 citations

Human-human hybridomas producing monoclonal antibodies of predefined antigenic specificity.

1980 • 383 citations

Polyclonal Ig production after Epstein-Barr virus infection of human lymphocytes in vitro

1977 • 378 citations

Chromosomal location of the genes for human immunoglobulin heavy chains.

1979 • 368 citations

Protection against gram-negative bacteremia and endotoxemia with human monoclonal IgM antibodies.

1985 • 316 citations

Production of human hybridomas secreting antibodies to measles virus

1980 • 308 citations

Introduction of a μ immunoglobulin gene into the mouse germ line: Specific expression in lymphoid cells and synthesis of functional antibody

1984 • 304 citations

Production of Free Light Chains of Immunoglobulin by a Hematopoietic Cell Line Derived from a Patient with Multiple Myeloma.

1967 • 294 citations

COLD PLATELET AGGLUTININS

1975 • 291 citations

Idiotypic cross-reactions of monoclonal human lupus autoantibodies.

1983 • 285 citations

Human lymphoblastoid lines from lymph node and spleen

1968 • 261 citations

Generation of human monoclonal antibodies reactive with human mammary carcinoma cells.

1980 • 255 citations

Monoclonal antibody production by receptor-mediated electrically induced cell fusion

1984 • 255 citations

Cyclosporin A promotes spontaneous outgrowth in vitro of Epstein–Barr virus-induced B-cell lines

1981 • 242 citations

Localization of human immunoglobulin kappa light chain variable region genes to the short arm of chromosome 2 by in situ hybridization.

1982 • 242 citations

Assignment of the genes for human λ immunoglobulin chains to chromosome 22

1981 • 239 citations

Naturally occurring anti-idiotypic antibodies in myasthenia gravis patients

1983 • 236 citations

Production of autoantibodies by human-human hybridomas.

1982 • 228 citations

ANTI-DNA ANTIBODY IDIOTYPES IN SYSTEMIC LUPUS ERYTHEMATOSUS

1984 • 221 citations

A human‐human hybridoma system based on a fast‐growing mutant of the ARH‐77 plasma cell leukemia‐derived line

1982 • 214 citations

Characterization of a Circulating Subpopulation of Spontaneous Antitetanus Toxoid Antibody Producing B Cells Following in Vivo Booster Immunization

1979 • 201 citations

gp140, the C3d receptor of human B lymphocytes, is also the Epstein-Barr virus receptor.

1985 • 193 citations

B cell growth factors and B cell differentiation factor from human T hybridomas. Two distinct kinds of B cell growth factor and their synergism in B cell proliferation.

1983 • 182 citations

Large-Scale Cultivation of Mammalian Cells

1970 • 175 citations

Construction and testing of mouse--human heteromyelomas for human monoclonal antibody production.

1983 • 168 citations

Assembly of functional antibodies from immunoglobulin heavy and light chains synthesised inE. coli

1984 • 165 citations

Generation of human monoclonal antibodies reactive with cellular antigens.

1983 • 164 citations

Human hybridomas constructed with antigen-specific Epstein-Barr virus-transformed cell lines.

1982 • 161 citations

Isolation and characterization of purine-nucleoside phosphorylase-deficient T-lymphoma cells and secondary mutants with altered ribonucleotide reductase: Genetic model for immunodeficiency disease

1979 • 158 citations

Requirements for the establishment of high-titered human monoclonal antibodies against tetanus toxoid using the Epstein-Barr virus technique.

1981 • 153 citations

UC 729-6, a human lymphoblastoid B-cell line useful for generating antibody-secreting human-human hybridomas.

1983 • 149 citations

Human Monoclonal Autoantibodies That React with Multiple Endocrine Organs

1983 • 145 citations

Anti-DNA autoantibody-producing hybridomas of normal human lymphoid cell origin.

1984 • 135 citations

Rescue of immunoglobulin secretion from human neoplastic lymphoid cells by somatic cell hybridization.

1978 • 131 citations

In vitro antigen-induced, antigen-specific antibody production in man. Specific and polyclonal components, kinetics, and cellular requirements.

1981 • 130 citations

Linkage and sequence homology of two human immunoglobulin gamma heavy chain constant region genes.

1982 • 126 citations

Functional immunoglobulin M production after transfection of cloned immunoglobulin heavy and light chain genes into lymphoid cells.

1983 • 121 citations

Amphotropic retrovirus vector system for human cell gene transfer.

1984 • 117 citations

High expression of cloned immunoglobulin k gene in transgenic mice is restricted to B lymphocytes

1984 • 114 citations

Human Monoclonal Antibody Against Forssman Antigen

1980 • 111 citations

Automated production of monoclonal antibodies in a cytostat

1983 • 110 citations

Detection of cell surface and intracellular antigens by human monoclonal antibodies. Hybrid cell lines derived from lymphocytes of patients with malignant melanoma.

1983 • 110 citations

Human × Mouse Somatic Cell Hybrid Clone secreting Immunoglobulins of both Parental Types

1973 • 110 citations

Use of Epstein-Barr virus-transformed B cell lines for the generation of immunoglobulin-producing human B cell hybridomas.

1982 • 106 citations

Effect of poly(ethylene glycol) on phospholipid hydration and polarity of the external phase

1983 • 106 citations

The production of monoclonal antibodies from human lymphocytes

1983 • 100 citations

Establishment of a human plasma cell line in vitro.

1978 • 99 citations

Human monoclonal antibodies to lung-cancer antigens

1981 • 98 citations

Biological and Clinical Implications of Lymphocyte Hybridomas: Tumor Therapy with Monoclonal Antibodies

1983 • 91 citations

ORGANIC ACIDURIAS

1975 • 88 citations

Production of functional human T-T hybridomas in selection medium lacking aminopterin and thymidine.

1982 • 88 citations

Preferential retention of human chromosome 14 in mouse × human B cell hybrids

1980 • 86 citations

Human Myeloma Cell Line Carrying a Philadelphia Chromosome

1982 • 84 citations

Determination of the optimal human cell lines for development of human hybridomas.

1983 • 83 citations

Rescue of human monoclonal antibody production from an EBV-transformed B cell line by fusion to a human-mouse hybridoma

1984 • 83 citations

Human X (Mouse X Human) Hybridomas Stably Producing Human Antibodies

1983 • 82 citations

Human monoclonal antibodies

1984 • 81 citations

Homology of the NH2-terminal amino acid sequences of the heavy and light chains of human monoclonal lupus autoantibodies containing the dominant 16/6 idiotype.

1985 • 79 citations

Transfer of a cloned immunoglobulin light-chain gene to mutant hybridoma cells restores specific antibody production

1983 • 78 citations

Immunoglobulin expression by human B lymphocytes clonally transformed by Epstein Barr virus.

1982 • 78 citations

Regulation of B Cell Activity in Man: Role of T Cells

1979 • 76 citations

Most κ immunoglobulin mRNA in human lymphocytes is homologous to a small family of germ-line V genes

1984 • 75 citations

Human monoclonal anti-keyhole limpet hemocyanin antibody-secreting hybridoma produced from peripheral blood B lymphocytes of a keyhole limpet hemocyanin-immune individual.

1982 • 72 citations

Antibody producing human-human hybridomas. I. Technical aspects

1983 • 71 citations

Human Anti-Tetanus Toxoid Monoclonal Antibody Secreted by EBV-Transformed Human B Cells Fused with Murine Myeloma

1982 • 70 citations

Characterization of human hybridomas secreting antibody to tetanus toxoid.

1983 • 70 citations

Somatic cell hybridization

1974 • 70 citations

Human hybridomas from patients with malignant disease

1983 • 69 citations

Human hybridomas secreting anti-islet autoantibodies

1982 • 67 citations

Monoclonal Antibodies in Cancer

1982 • 65 citations

Protective human hybridoma antibody to tetanus toxin.

1982 • 65 citations

Linkage of cytotoxic agents to immunoglobulins

1983 • 63 citations

Production of Human IgM Anti-D in Tissue Culture by EB-Virus-transformed Lymphocytes

1980 • 61 citations

Establishment and characterization of a human myeloma cell line (KMM‐1)

1982 • 61 citations

Delineation of optimal conditions for producing mouse-human heterohybridomas from human peripheral blood B cells of immunized subjects.

1983 • 59 citations

Mapping of the Human Blym -1 Transforming Gene Activated in Burkitt Lymphomas to Chromosome 1

1984 • 59 citations

Efficient generation in vitro, from human peripheral blood cells, of monoclonal Epstein-Barr virus transformants producing specific antibody to a variety of antigens without prior deliberate immunization.

1983 • 59 citations

Frequencies of the separate human B cell subsets activatable to Ig secretion by Epstein-Barr virus and pokeweed mitogen.

1983 • 56 citations

Regulation of in vitro primary anti-DNP antibody production by functional subsets of T lymphocytes in man.

1981 • 55 citations

Homokaryon production by electrofusion: A convenient way to produce a large number of viable mammalian fused cells

1983 • 53 citations

Expression of an immunoglobulin heavy chain gene transfected into lymphocytes.

1984 • 53 citations

Establishment and characterization of a human non‐secretory plasmacytoid cell line and its hybridization with human B cells

1983 • 52 citations

Human monoclonal autoimmune antibody produced in Vitro: rheumatoid factor generated by Epstein‐Barr virus‐transformed cell line

1982 • 52 citations

Hybridoma anti-DNA autoantibodies from patients with rheumatoid arthritis and systemic lupus erythematosus demonstrate similar nucleic acid binding characteristics.

1985 • 49 citations

The effect of 5-bromouracil deoxyriboside on the synthesis of antibody in vitro

1960 • 48 citations

Properties of a human immunoglobulin epsilon-chain fragment synthesized in Escherichia coli.

1984 • 48 citations

Expression of Purine Overproduction in a Series of 8-Azaguanine-Resistant Diploid Human Lymphoblast Lines

1974 • 47 citations

A human monoclonal antibody to cytomegalovirus (CMV).

1984 • 45 citations

HUMAN PLASMACYTOMA WITH AN UNUSUAL KARYOTYPE GROWING IN VITRO AND PRODUCING LIGHT-CHAIN IMMUNOGLOBULIN

1982 • 44 citations

In vitro stimulated lymphocytes as a source of human hybridomas

1984 • 44 citations

Reproducible Production of Protective Human Monoclonal Antibodies by Fusion of Peripheral Blood Lymphocytes with a Mouse Myeloma Cell Line

1984 • 42 citations

Gene Dose Effect: Regional Mapping of Human Nucleoside Phosphorylase on Chromosome 14

1976 • 42 citations

ANTIBACTERIAL ACTIVITY OF A HUMAN MONOCLONAL ANTIBODY TO HAEMOPHILUS INFLUENZAE TYPE B CAPSULAR POLYSACCHARIDE

1982 • 42 citations

Immunological Tolerance Affects Only a Subpopulation of the Antigen‐Specific B Lymphocytes: Evidence against Clonal Deletion as the Mechanism of Tolerance Induction

1979 • 41 citations

Immunoglobulin production by a human-mouse somatic cell hybrid

1975 • 37 citations

Production of large quantities of human immunoglobulin in the ascites of athymic mice: implications for the development of anti-human idiotype monoclonal antibodies.

1984 • 36 citations

Transfer and Expression of Immunoglobulin Genes

1984 • 36 citations

Metabolism to methionine and growth stimulation by 5′-methylthioadenosine and 5′-methylthioinosine in mammalian cells

1983 • 31 citations

Ca2+-induced fusion of avian erythrocytes

1971 • 31 citations

Selective induction of autoantibody secretion in human bone marrow by Epstein Barr virus.

1982 • 31 citations

Human monoclonal antibodies to glioma cells

1981 • 30 citations

Influence of Fusion Cell Ratio and Cell Plating Density on Production of Human-Human Hybridomas Secreting Anti-DNA Autoantibodies from Patients with Systemic Lupus Erythematosus

1984 • 29 citations

Human B-lymphocyte Function: Activation and Immunoregulation

1982 • 27 citations

Production of Human Monoclonal Antibody in Mouse Ascites

1984 • 27 citations

A Comparative Analysis of the Phenotypic Characteristics of Available Fusion Partners for the Construction of Human Hybridomas

1983 • 27 citations

Further characterization of cooperative interactions of monoclonal antibodies.

1983 • 26 citations

Induction of human immunoglobulin synthesis and secretion in somatic cell hybrids of mouse myeloma and human B lymphocytes from patients with agammaglobulinemia.

1978 • 26 citations

Induction of Reaginic Hypersensitivity

1979 • 24 citations

Increase of hybridoma formation by human lymphocytes after stimulation in vitro; effect of antigen, endothelial cells, and PWM.

1982 • 23 citations

Different populations of rheumatoid factor idiotypes induced by two polyclonal B cell activators, pokeweed mitogen and Epstein-Barr virus

1981 • 22 citations

Human-human hybrids secreting pneumococcal antibodies

1984 • 22 citations

Production of Continuous Mouse Plasma Cell Lines by Transfection with Human Leukemia DNA

1984 • 20 citations

Monoclonal lupus autoantibody secretion by human-human hybridomas. Selection of hybrids by conventional and novel techniques.

1983 • 20 citations

Human-human B cell hybridomas from in vitro stimulated lymphocytes of patients with common variable immunodeficiency.

1983 • 20 citations

Frequencies of epstein‐barr virus—inducible IgM anti‐IgG B lymphocytes in normal children and children with juvenile rheumatoid arthritis

1982 • 20 citations

Somatic cell hybrid selection with a transfectable dominant marker

1984 • 19 citations

A simple method for fusing human lymphocytes with rodent cells in monolayer by polyethylene glycol

1981 • 17 citations

Chemical typing of human kappa light chain subgroups expressed by human hybrid myelomas.

1982 • 17 citations

Fractionation of human lymphocyte subpopulations on immunoglobulin coated petri dishes

1981 • 16 citations

Establishment of Autoantibody‐Producing Cell Lines from Peripheral Blood Lymphocytes of Patients with Systemic Lupus Erythematosus

1980 • 16 citations

Molecular interactions in human T-cell-mediated cytotoxicity to Epstein-Barr virus

1982 • 15 citations

Dynamics of human circulating antigen reactive cells following secondary immunization with tetanus toxoid

1981 • 15 citations

Human T cell hybridomas specific for Epstein Barr virus-infected B lymphocytes.

1983 • 14 citations

Prospects for human monoclonal antibodies: a critical perspective.

1982 • 8 citations

In Vivo Production of Human Hybridorna Antibody to the Haemophilus influenzae B Capsule in Athymic Nude Mice

1984 • 4 citations

Evidence for a trans-dominant regulator of purine nucleoside phosphorylase expression in rat hepatoma cells

1983 • 1 citations