Deletion of MUD2, the yeast homolog of U2AF65, can bypass the requirement for Sub2, an essential spliceosomal ATPase

A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae.

1989 • 8,705 citations

Additional modules for versatile and economical PCR-based gene deletion and modification in Saccharomyces cerevisiae

1998 • 5,253 citations

Mechanical Devices of the Spliceosome: Motors, Clocks, Springs, and Things

1998 • 1,121 citations

Crystal Structures of Complexes of PcrA DNA Helicase with a DNA Substrate Indicate an Inchworm Mechanism

1999 • 793 citations

A method for random mutagenesis of a defined DNA segment using a modified polymerase chain reaction

1989 • 614 citations

Mutational analysis of a DEAD box RNA helicase: the mammalian translation initiation factor eIF-4A.

1992 • 613 citations

A factor, U2AF, is required for U2 snRNP binding and splicing complex assembly

1988 • 535 citations

Interactions between small nuclear ribonucleoprotein particles in formation of spliceosomes

1987 • 530 citations

Electrophoretic separation of complexes involved in the splicing of precursors to mRNAs

1986 • 490 citations

Major Domain Swiveling Revealed by the Crystal Structures of Complexes of E. coli Rep Helicase Bound to Single-Stranded DNA and ADP

1997 • 490 citations

A rapid method for localized mutagenesis of yeast genes

1992 • 456 citations

Structure of the hepatitis C virus RNA helicase domain

1997 • 444 citations

Spliceosome assembly in yeast.

1987 • 402 citations

Cross-Intron Bridging Interactions in the Yeast Commitment Complex Are Conserved in Mammals

1997 • 342 citations

An RNA Switch at the 5′ Splice Site Requires ATP and the DEAD Box Protein Prp28p

1999 • 330 citations

The Splicing Factor BBP Interacts Specifically with the Pre-mRNA Branchpoint Sequence UACUAAC

1997 • 327 citations

An ATP-independent complex commits pre-mRNA to the mammalian spliceosome assembly pathway.

1991 • 297 citations

Construction of a GAL1-regulated yeast cDNA expression library and its application to the identification of genes whose overexpression causes lethality in yeast.

1992 • 284 citations

The HRIGRXXR region of the DEAD box RNA helicase eukaryotic translation initiation factor 4A is required for RNA binding and ATP hydrolysis.

1993 • 280 citations

U2AF65 recruits a novel human DEAD box protein required for the U2 snRNP-branchpoint interaction.

1997 • 277 citations

A cooperative interaction between U2AF65 and mBBP/SF1 facilitates branchpoint region recognition

1998 • 253 citations

Specific Alterations of U1-C Protein or U1 Small Nuclear RNA Can Eliminate the Requirement of Prp28p, an Essential DEAD Box Splicing Factor

2001 • 201 citations

Functional Association of U2 snRNP with the ATP-Independent Spliceosomal Complex E

2000 • 192 citations

The yeast MUD2 protein: an interaction with PRP11 defines a bridge between commitment complexes and U2 snRNP addition.

1994 • 188 citations

The DEAH-box protein PRP22 is an ATPase that mediates ATP-dependent mRNA release from the spliceosome and unwinds RNA duplexes

1998 • 164 citations

Helicases: a unifying structural theme?

1998 • 162 citations

Four yeast spliceosomal proteins (PRP5, PRP9, PRP11, and PRP21) interact to promote U2 snRNP binding to pre-mRNA.

1993 • 142 citations

The Saccharomyces cerevisiae Prp5 Protein Has RNA-dependent ATPase Activity with Specificity for U2 Small Nuclear RNA

1996 • 120 citations

Multiple roles for the yeast SUB2/yUAP56 gene in splicing

2001 • 118 citations

Comparisons between the structures of HCV and Rep helicases reveal structural similarities between SF1 and SF2 super‐families of helicases

1998 • 117 citations

Identification and characterization of yUAP/Sub2p, a yeast homolog of the essential human pre-mRNA splicing factor hUAP56

2001 • 116 citations

Three protein factors (SF1, SF3 and U2AF) function in pre-splicing complex formation in addition to snRNPs.

1991 • 104 citations

The DEAH-box splicing factor Prp16 unwinds RNA duplexes in vitro

1998 • 99 citations

A dual role for BBP/ScSF1 in nuclear pre-mRNA retention and splicing

2000 • 98 citations

Transient interaction of BBP/ScSF1 and Mud2 with the splicing machinery affects the kinetics of spliceosome assembly

1999 • 95 citations

In vitro reconstitution of functional yeast U2 snRNPs.

1989 • 91 citations

Mutagenesis of the Yeast Gene PRP8 Reveals Domains Governing the Specificity and Fidelity of 3′ Splice Site Selection

1996 • 85 citations

Human homologs of yeast Prp16 and Prp17 reveal conservation of the mechanism for catalytic step II of pre-mRNA splicing

1998 • 78 citations

Identification of a putative RNA helicase (HRH1), a human homolog of yeast Prp22.

1994 • 75 citations

Crystallographic structure of the amino terminal domain of yeast initiation factor 4A, a representative DEAD-box RNA helicase

1999 • 74 citations

ATP can be dispensable for prespliceosome formation in yeast

2000 • 67 citations

Requirements for U2 snRNP addition to yeast pre-mRNA

1992 • 58 citations

Transcriptional pulse-chase analysis reveals a role for a novel snRNP-associated protein in the manufacture of spliceosomal snRNPs.

1996 • 56 citations

Mutational Analysis of the Yeast DEAH-Box Splicing Factor Prp16

1998 • 51 citations

In Vitro Studies of the Prp9·Prp11·Prp21 Complex Indicate a Pathway for U2 Small Nuclear Ribonucleoprotein Activation

1996 • 44 citations

Dominant negative mutants of the yeast splicing factor Prp2 map to a putative cleft region in the helicase domain of DExD/H-box proteins

2000 • 39 citations

Cloning of mDEAH9, a putative RNA helicase and mammalian homologue of Saccharomyces cerevisiae splicing factor Prp43

1997 • 28 citations

Disruption of sixSaccharomyces cerevisiae genes from chromosome IV and basic phenotypic analysis of deletion mutants

1998 • 12 citations